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Astragalus Polysaccharide Promotes Adriamycin-Induced Apoptosis in Gastric Cancer Cells
PURPOSE: Astragalus polysaccharide (APS), a common Chinese herbal compound extracted from Astragalus membranaceus, has been proposed to increase the tumour response of and stabilize chemotherapy drugs while reducing their toxicity. Here, we examined the effects of APS on apoptosis in gastric cancer...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131994/ https://www.ncbi.nlm.nih.gov/pubmed/32280276 http://dx.doi.org/10.2147/CMAR.S237146 |
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author | Song, Jie Chen, Youming He, Donghong Tan, Wenhui Lv, Fang Liang, Biao Xia, Tingting Li, Jing |
author_facet | Song, Jie Chen, Youming He, Donghong Tan, Wenhui Lv, Fang Liang, Biao Xia, Tingting Li, Jing |
author_sort | Song, Jie |
collection | PubMed |
description | PURPOSE: Astragalus polysaccharide (APS), a common Chinese herbal compound extracted from Astragalus membranaceus, has been proposed to increase the tumour response of and stabilize chemotherapy drugs while reducing their toxicity. Here, we examined the effects of APS on apoptosis in gastric cancer (GC) cells in the presence or absence of adriamycin (0.1 µg/mL). METHODS: GC cells cultured in the presence or absence of adriamycin (0.1 µg/mL) were administered APS (50–200 µg/mL) for 24–72 h and subjected to an MTT assay to examine cell viability. Active caspase-3 expression and DNA fragmentation were assessed to evaluate apoptosis, and real-time PCR was used to analyse the expression levels of multidrug resistance (MDR1) genes and tumour suppressor genes. Western blot analysis was applied to detect cleaved caspase-3 and phosphorylated AMPK (p-AMPK). RESULTS: Cellular viability was profoundly reduced by APS, and GC cell apoptosis was strongly increased by APS in a time- and dose-dependent manner; these changes may be linked to an increase in p-AMPK levels because the AMPK inhibitor compound C blocked the effects of APS. Similarly, adriamycin-induced decreases in cellular viability and apoptosis of GC cells were enhanced by APS administration. The expression of tumour suppressor genes (SEMA3F, P21(WAF1/CIP1), FBXW7), but not of MDR1, was increased by APS compared to the control, and p-AMPK levels were lower in adriamycin-resistant GC cells than in either adriamycin-sensitive GC cells or an immortalized human gastric epithelial cell line. CONCLUSION: APS induces apoptosis independently and strengthens the proapoptotic effect of adriamycin on GC cells, suggesting that APS may act as a chemotherapeutic sensitizer. |
format | Online Article Text |
id | pubmed-7131994 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-71319942020-04-10 Astragalus Polysaccharide Promotes Adriamycin-Induced Apoptosis in Gastric Cancer Cells Song, Jie Chen, Youming He, Donghong Tan, Wenhui Lv, Fang Liang, Biao Xia, Tingting Li, Jing Cancer Manag Res Original Research PURPOSE: Astragalus polysaccharide (APS), a common Chinese herbal compound extracted from Astragalus membranaceus, has been proposed to increase the tumour response of and stabilize chemotherapy drugs while reducing their toxicity. Here, we examined the effects of APS on apoptosis in gastric cancer (GC) cells in the presence or absence of adriamycin (0.1 µg/mL). METHODS: GC cells cultured in the presence or absence of adriamycin (0.1 µg/mL) were administered APS (50–200 µg/mL) for 24–72 h and subjected to an MTT assay to examine cell viability. Active caspase-3 expression and DNA fragmentation were assessed to evaluate apoptosis, and real-time PCR was used to analyse the expression levels of multidrug resistance (MDR1) genes and tumour suppressor genes. Western blot analysis was applied to detect cleaved caspase-3 and phosphorylated AMPK (p-AMPK). RESULTS: Cellular viability was profoundly reduced by APS, and GC cell apoptosis was strongly increased by APS in a time- and dose-dependent manner; these changes may be linked to an increase in p-AMPK levels because the AMPK inhibitor compound C blocked the effects of APS. Similarly, adriamycin-induced decreases in cellular viability and apoptosis of GC cells were enhanced by APS administration. The expression of tumour suppressor genes (SEMA3F, P21(WAF1/CIP1), FBXW7), but not of MDR1, was increased by APS compared to the control, and p-AMPK levels were lower in adriamycin-resistant GC cells than in either adriamycin-sensitive GC cells or an immortalized human gastric epithelial cell line. CONCLUSION: APS induces apoptosis independently and strengthens the proapoptotic effect of adriamycin on GC cells, suggesting that APS may act as a chemotherapeutic sensitizer. Dove 2020-04-01 /pmc/articles/PMC7131994/ /pubmed/32280276 http://dx.doi.org/10.2147/CMAR.S237146 Text en © 2020 Song et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Song, Jie Chen, Youming He, Donghong Tan, Wenhui Lv, Fang Liang, Biao Xia, Tingting Li, Jing Astragalus Polysaccharide Promotes Adriamycin-Induced Apoptosis in Gastric Cancer Cells |
title | Astragalus Polysaccharide Promotes Adriamycin-Induced Apoptosis in Gastric Cancer Cells |
title_full | Astragalus Polysaccharide Promotes Adriamycin-Induced Apoptosis in Gastric Cancer Cells |
title_fullStr | Astragalus Polysaccharide Promotes Adriamycin-Induced Apoptosis in Gastric Cancer Cells |
title_full_unstemmed | Astragalus Polysaccharide Promotes Adriamycin-Induced Apoptosis in Gastric Cancer Cells |
title_short | Astragalus Polysaccharide Promotes Adriamycin-Induced Apoptosis in Gastric Cancer Cells |
title_sort | astragalus polysaccharide promotes adriamycin-induced apoptosis in gastric cancer cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131994/ https://www.ncbi.nlm.nih.gov/pubmed/32280276 http://dx.doi.org/10.2147/CMAR.S237146 |
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