Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells

A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to...

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Autores principales: Matsuyama, Shutoku, Nao, Naganori, Shirato, Kazuya, Kawase, Miyuki, Saito, Shinji, Takayama, Ikuyo, Nagata, Noriyo, Sekizuka, Tsuyoshi, Katoh, Hiroshi, Kato, Fumihiro, Sakata, Masafumi, Tahara, Maino, Kutsuna, Satoshi, Ohmagari, Norio, Kuroda, Makoto, Suzuki, Tadaki, Kageyama, Tsutomu, Takeda, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2020
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132130/
https://www.ncbi.nlm.nih.gov/pubmed/32165541
http://dx.doi.org/10.1073/pnas.2002589117
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author Matsuyama, Shutoku
Nao, Naganori
Shirato, Kazuya
Kawase, Miyuki
Saito, Shinji
Takayama, Ikuyo
Nagata, Noriyo
Sekizuka, Tsuyoshi
Katoh, Hiroshi
Kato, Fumihiro
Sakata, Masafumi
Tahara, Maino
Kutsuna, Satoshi
Ohmagari, Norio
Kuroda, Makoto
Suzuki, Tadaki
Kageyama, Tsutomu
Takeda, Makoto
author_facet Matsuyama, Shutoku
Nao, Naganori
Shirato, Kazuya
Kawase, Miyuki
Saito, Shinji
Takayama, Ikuyo
Nagata, Noriyo
Sekizuka, Tsuyoshi
Katoh, Hiroshi
Kato, Fumihiro
Sakata, Masafumi
Tahara, Maino
Kutsuna, Satoshi
Ohmagari, Norio
Kuroda, Makoto
Suzuki, Tadaki
Kageyama, Tsutomu
Takeda, Makoto
author_sort Matsuyama, Shutoku
collection PubMed
description A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.
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spelling pubmed-71321302020-04-09 Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells Matsuyama, Shutoku Nao, Naganori Shirato, Kazuya Kawase, Miyuki Saito, Shinji Takayama, Ikuyo Nagata, Noriyo Sekizuka, Tsuyoshi Katoh, Hiroshi Kato, Fumihiro Sakata, Masafumi Tahara, Maino Kutsuna, Satoshi Ohmagari, Norio Kuroda, Makoto Suzuki, Tadaki Kageyama, Tsutomu Takeda, Makoto Proc Natl Acad Sci U S A Biological Sciences A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2. National Academy of Sciences 2020-03-31 2020-03-12 /pmc/articles/PMC7132130/ /pubmed/32165541 http://dx.doi.org/10.1073/pnas.2002589117 Text en Copyright © 2020 the Author(s). Published by PNAS. http://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biological Sciences
Matsuyama, Shutoku
Nao, Naganori
Shirato, Kazuya
Kawase, Miyuki
Saito, Shinji
Takayama, Ikuyo
Nagata, Noriyo
Sekizuka, Tsuyoshi
Katoh, Hiroshi
Kato, Fumihiro
Sakata, Masafumi
Tahara, Maino
Kutsuna, Satoshi
Ohmagari, Norio
Kuroda, Makoto
Suzuki, Tadaki
Kageyama, Tsutomu
Takeda, Makoto
Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells
title Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells
title_full Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells
title_fullStr Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells
title_full_unstemmed Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells
title_short Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells
title_sort enhanced isolation of sars-cov-2 by tmprss2-expressing cells
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132130/
https://www.ncbi.nlm.nih.gov/pubmed/32165541
http://dx.doi.org/10.1073/pnas.2002589117
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