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Protective effects of fisetin against myocardial ischemia/reperfusion injury

The underlying mechanism of the myocardial protective effect of fisetin was studied in a rat ischemia/reperfusion injury model. Sprague-Dawley rats were randomly assigned to seven groups and pretreated with different solutions by gavage administration. A rat model of cardiac ischemia/reperfusion inj...

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Autores principales: Long, Lihui, Han, Xuliang, Ma, Xingming, Li, Kai, Liu, Linjie, Dong, Juanni, Qin, Bei, Zhang, Kelin, Yang, Kuan, Yan, Honglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132235/
https://www.ncbi.nlm.nih.gov/pubmed/32266013
http://dx.doi.org/10.3892/etm.2020.8576
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author Long, Lihui
Han, Xuliang
Ma, Xingming
Li, Kai
Liu, Linjie
Dong, Juanni
Qin, Bei
Zhang, Kelin
Yang, Kuan
Yan, Honglin
author_facet Long, Lihui
Han, Xuliang
Ma, Xingming
Li, Kai
Liu, Linjie
Dong, Juanni
Qin, Bei
Zhang, Kelin
Yang, Kuan
Yan, Honglin
author_sort Long, Lihui
collection PubMed
description The underlying mechanism of the myocardial protective effect of fisetin was studied in a rat ischemia/reperfusion injury model. Sprague-Dawley rats were randomly assigned to seven groups and pretreated with different solutions by gavage administration. A rat model of cardiac ischemia/reperfusion injury was established. Plasma levels of Von Willebrand factor (vWF) were determined by ELISA, flow cytometry was used to determine the level of cardiomyocyte apoptosis and 2,3,5-triphenyltetrazolium staining was used to determine the size of myocardial infarcts. Hematoxylin and eosin-stained sections of myocardial tissues were examined for pathological changes. Expressions of nuclear factor (NF)-κB and matrix metallopeptidase 9 (MMP-9) were measured by immunohistochemistry. Compared with the model group, rats pretreated with fisetin, quercetin and aspirin showed significant prolongation of clotting time, prothrombin time, thrombin time and activated partial thromboplastin time. Fisetin treatment better maintained the integrity of myocardial fibers and nuclear integrity, reduced the percentage of apoptotic myocardial cells, inhibited expression of NF-κB, decreased the loss of MMP-9 and reduced nuclear translocation of NF-kB. Rats pretreated with fisetin also demonstrated a significant decrease in plasma levels of vWF. In addition, the protective effect of fisetin on myocardial cells was found to be dose dependent.
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spelling pubmed-71322352020-04-07 Protective effects of fisetin against myocardial ischemia/reperfusion injury Long, Lihui Han, Xuliang Ma, Xingming Li, Kai Liu, Linjie Dong, Juanni Qin, Bei Zhang, Kelin Yang, Kuan Yan, Honglin Exp Ther Med Articles The underlying mechanism of the myocardial protective effect of fisetin was studied in a rat ischemia/reperfusion injury model. Sprague-Dawley rats were randomly assigned to seven groups and pretreated with different solutions by gavage administration. A rat model of cardiac ischemia/reperfusion injury was established. Plasma levels of Von Willebrand factor (vWF) were determined by ELISA, flow cytometry was used to determine the level of cardiomyocyte apoptosis and 2,3,5-triphenyltetrazolium staining was used to determine the size of myocardial infarcts. Hematoxylin and eosin-stained sections of myocardial tissues were examined for pathological changes. Expressions of nuclear factor (NF)-κB and matrix metallopeptidase 9 (MMP-9) were measured by immunohistochemistry. Compared with the model group, rats pretreated with fisetin, quercetin and aspirin showed significant prolongation of clotting time, prothrombin time, thrombin time and activated partial thromboplastin time. Fisetin treatment better maintained the integrity of myocardial fibers and nuclear integrity, reduced the percentage of apoptotic myocardial cells, inhibited expression of NF-κB, decreased the loss of MMP-9 and reduced nuclear translocation of NF-kB. Rats pretreated with fisetin also demonstrated a significant decrease in plasma levels of vWF. In addition, the protective effect of fisetin on myocardial cells was found to be dose dependent. D.A. Spandidos 2020-05 2020-03-06 /pmc/articles/PMC7132235/ /pubmed/32266013 http://dx.doi.org/10.3892/etm.2020.8576 Text en Copyright: © Long et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Long, Lihui
Han, Xuliang
Ma, Xingming
Li, Kai
Liu, Linjie
Dong, Juanni
Qin, Bei
Zhang, Kelin
Yang, Kuan
Yan, Honglin
Protective effects of fisetin against myocardial ischemia/reperfusion injury
title Protective effects of fisetin against myocardial ischemia/reperfusion injury
title_full Protective effects of fisetin against myocardial ischemia/reperfusion injury
title_fullStr Protective effects of fisetin against myocardial ischemia/reperfusion injury
title_full_unstemmed Protective effects of fisetin against myocardial ischemia/reperfusion injury
title_short Protective effects of fisetin against myocardial ischemia/reperfusion injury
title_sort protective effects of fisetin against myocardial ischemia/reperfusion injury
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132235/
https://www.ncbi.nlm.nih.gov/pubmed/32266013
http://dx.doi.org/10.3892/etm.2020.8576
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