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Antiviral activity of carbohydrate-binding agents against Nidovirales in cell culture
Coronaviruses are important human and animal pathogens, the relevance of which increased due to the emergence of new human coronaviruses like SARS-CoV, HKU1 and NL63. Together with toroviruses, arteriviruses, and roniviruses the coronaviruses belong to the order Nidovirales. So far antivirals are ha...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132385/ https://www.ncbi.nlm.nih.gov/pubmed/17560666 http://dx.doi.org/10.1016/j.antiviral.2007.04.003 |
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author | van der Meer, F.J.U.M. de Haan, C.A.M. Schuurman, N.M.P. Haijema, B.J. Peumans, W.J. Van Damme, E.J.M. Delputte, P.L. Balzarini, J. Egberink, H.F. |
author_facet | van der Meer, F.J.U.M. de Haan, C.A.M. Schuurman, N.M.P. Haijema, B.J. Peumans, W.J. Van Damme, E.J.M. Delputte, P.L. Balzarini, J. Egberink, H.F. |
author_sort | van der Meer, F.J.U.M. |
collection | PubMed |
description | Coronaviruses are important human and animal pathogens, the relevance of which increased due to the emergence of new human coronaviruses like SARS-CoV, HKU1 and NL63. Together with toroviruses, arteriviruses, and roniviruses the coronaviruses belong to the order Nidovirales. So far antivirals are hardly available to combat infections with viruses of this order. Therefore, various antiviral strategies to counter nidoviral infections are under evaluation. Lectins, which bind to N-linked oligosaccharide elements of enveloped viruses, can be considered as a conceptionally new class of virus inhibitors. These agents were recently evaluated for their antiviral activity towards a variety of enveloped viruses and were shown in most cases to inhibit virus infection at low concentrations. However, limited knowledge is available for their efficacy towards nidoviruses. In this article the application of the plant lectins Hippeastrum hybrid agglutinin (HHA), Galanthus nivalis agglutinin (GNA), Cymbidium sp. agglutinin (CA) and Urtica dioica agglutinin (UDA) as well as non-plant derived pradimicin-A (PRM-A) and cyanovirin-N (CV-N) as potential antiviral agents was evaluated. Three antiviral tests were compared based on different evaluation principles: cell viability (MTT-based colorimetric assay), number of infected cells (immunoperoxidase assay) and amount of viral protein expression (luciferase-based assay). The presence of carbohydrate-binding agents strongly inhibited coronaviruses (transmissible gastroenteritis virus, infectious bronchitis virus, feline coronaviruses serotypes I and II, mouse hepatitis virus), arteriviruses (equine arteritis virus and porcine respiratory and reproductive syndrome virus) and torovirus (equine Berne virus). Remarkably, serotype II feline coronaviruses and arteriviruses were not inhibited by PRM-A, in contrast to the other viruses tested. |
format | Online Article Text |
id | pubmed-7132385 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71323852020-04-08 Antiviral activity of carbohydrate-binding agents against Nidovirales in cell culture van der Meer, F.J.U.M. de Haan, C.A.M. Schuurman, N.M.P. Haijema, B.J. Peumans, W.J. Van Damme, E.J.M. Delputte, P.L. Balzarini, J. Egberink, H.F. Antiviral Res Article Coronaviruses are important human and animal pathogens, the relevance of which increased due to the emergence of new human coronaviruses like SARS-CoV, HKU1 and NL63. Together with toroviruses, arteriviruses, and roniviruses the coronaviruses belong to the order Nidovirales. So far antivirals are hardly available to combat infections with viruses of this order. Therefore, various antiviral strategies to counter nidoviral infections are under evaluation. Lectins, which bind to N-linked oligosaccharide elements of enveloped viruses, can be considered as a conceptionally new class of virus inhibitors. These agents were recently evaluated for their antiviral activity towards a variety of enveloped viruses and were shown in most cases to inhibit virus infection at low concentrations. However, limited knowledge is available for their efficacy towards nidoviruses. In this article the application of the plant lectins Hippeastrum hybrid agglutinin (HHA), Galanthus nivalis agglutinin (GNA), Cymbidium sp. agglutinin (CA) and Urtica dioica agglutinin (UDA) as well as non-plant derived pradimicin-A (PRM-A) and cyanovirin-N (CV-N) as potential antiviral agents was evaluated. Three antiviral tests were compared based on different evaluation principles: cell viability (MTT-based colorimetric assay), number of infected cells (immunoperoxidase assay) and amount of viral protein expression (luciferase-based assay). The presence of carbohydrate-binding agents strongly inhibited coronaviruses (transmissible gastroenteritis virus, infectious bronchitis virus, feline coronaviruses serotypes I and II, mouse hepatitis virus), arteriviruses (equine arteritis virus and porcine respiratory and reproductive syndrome virus) and torovirus (equine Berne virus). Remarkably, serotype II feline coronaviruses and arteriviruses were not inhibited by PRM-A, in contrast to the other viruses tested. Elsevier B.V. 2007-10 2007-05-21 /pmc/articles/PMC7132385/ /pubmed/17560666 http://dx.doi.org/10.1016/j.antiviral.2007.04.003 Text en Copyright © 2007 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article van der Meer, F.J.U.M. de Haan, C.A.M. Schuurman, N.M.P. Haijema, B.J. Peumans, W.J. Van Damme, E.J.M. Delputte, P.L. Balzarini, J. Egberink, H.F. Antiviral activity of carbohydrate-binding agents against Nidovirales in cell culture |
title | Antiviral activity of carbohydrate-binding agents against Nidovirales in cell culture |
title_full | Antiviral activity of carbohydrate-binding agents against Nidovirales in cell culture |
title_fullStr | Antiviral activity of carbohydrate-binding agents against Nidovirales in cell culture |
title_full_unstemmed | Antiviral activity of carbohydrate-binding agents against Nidovirales in cell culture |
title_short | Antiviral activity of carbohydrate-binding agents against Nidovirales in cell culture |
title_sort | antiviral activity of carbohydrate-binding agents against nidovirales in cell culture |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132385/ https://www.ncbi.nlm.nih.gov/pubmed/17560666 http://dx.doi.org/10.1016/j.antiviral.2007.04.003 |
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