FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens

The BioFire FilmArray Respiratory Panel (FA RP) is a rapid multiplexed molecular assay approved for detection of viral and atypical bacterial pathogens in nasopharyngeal specimens. This study aimed to evaluate the performance of the BioFire FilmArray Respiratory Panel v1.7 on bronchoscopy specimens....

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Autores principales: Locher, Kerstin, Roscoe, Diane, Jassem, Agatha, Wong, Titus, Hoang, Linda M.N., Charles, Marthe, Bryce, Elizabeth, Grant, Jennifer, Stefanovic, Aleksandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2019
Materias:
Clinical Study
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132745/
https://www.ncbi.nlm.nih.gov/pubmed/31607515
http://dx.doi.org/10.1016/j.diagmicrobio.2019.114880
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author Locher, Kerstin
Roscoe, Diane
Jassem, Agatha
Wong, Titus
Hoang, Linda M.N.
Charles, Marthe
Bryce, Elizabeth
Grant, Jennifer
Stefanovic, Aleksandra
author_facet Locher, Kerstin
Roscoe, Diane
Jassem, Agatha
Wong, Titus
Hoang, Linda M.N.
Charles, Marthe
Bryce, Elizabeth
Grant, Jennifer
Stefanovic, Aleksandra
author_sort Locher, Kerstin
collection PubMed
description The BioFire FilmArray Respiratory Panel (FA RP) is a rapid multiplexed molecular assay approved for detection of viral and atypical bacterial pathogens in nasopharyngeal specimens. This study aimed to evaluate the performance of the BioFire FilmArray Respiratory Panel v1.7 on bronchoscopy specimens. We tested 133 bronchial specimens (87 archived and 46 prospectively collected) with the FA RP and compared the results to the Luminex NxTAG Respiratory Pathogen Panel (NxTAG RPP). After discordant analysis, 123 specimens gave concordant results using the FA RP and the NxTAG RPP for an overall agreement of 93.9% (kappa = 0.88 [95% CI 0.80–0.96]), a positive percent agreement of 93.7% (95% CI 83.7–97.7) and a negative percent agreement of 94.1% (95% CI 84.9–98.1). In conclusion, the BioFire FilmArray RP performed reliably to detect a broad range of respiratory pathogens in bronchoscopy specimens.
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spelling pubmed-71327452020-04-08 FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens Locher, Kerstin Roscoe, Diane Jassem, Agatha Wong, Titus Hoang, Linda M.N. Charles, Marthe Bryce, Elizabeth Grant, Jennifer Stefanovic, Aleksandra Diagn Microbiol Infect Dis Clinical Study The BioFire FilmArray Respiratory Panel (FA RP) is a rapid multiplexed molecular assay approved for detection of viral and atypical bacterial pathogens in nasopharyngeal specimens. This study aimed to evaluate the performance of the BioFire FilmArray Respiratory Panel v1.7 on bronchoscopy specimens. We tested 133 bronchial specimens (87 archived and 46 prospectively collected) with the FA RP and compared the results to the Luminex NxTAG Respiratory Pathogen Panel (NxTAG RPP). After discordant analysis, 123 specimens gave concordant results using the FA RP and the NxTAG RPP for an overall agreement of 93.9% (kappa = 0.88 [95% CI 0.80–0.96]), a positive percent agreement of 93.7% (95% CI 83.7–97.7) and a negative percent agreement of 94.1% (95% CI 84.9–98.1). In conclusion, the BioFire FilmArray RP performed reliably to detect a broad range of respiratory pathogens in bronchoscopy specimens. Elsevier Inc. 2019-12 2019-08-02 /pmc/articles/PMC7132745/ /pubmed/31607515 http://dx.doi.org/10.1016/j.diagmicrobio.2019.114880 Text en © 2019 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Clinical Study
Locher, Kerstin
Roscoe, Diane
Jassem, Agatha
Wong, Titus
Hoang, Linda M.N.
Charles, Marthe
Bryce, Elizabeth
Grant, Jennifer
Stefanovic, Aleksandra
FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens
title FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens
title_full FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens
title_fullStr FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens
title_full_unstemmed FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens
title_short FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens
title_sort filmarray respiratory panel assay: an effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens
topic Clinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132745/
https://www.ncbi.nlm.nih.gov/pubmed/31607515
http://dx.doi.org/10.1016/j.diagmicrobio.2019.114880
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