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Tobacco, a highly efficient green bioreactor for production of therapeutic proteins

Molecular farming of pharmaceuticals in plants has the potential to provide almost unlimited amounts of recombinant proteins for use in disease diagnosis, prevention or treatment. Tobacco has been and will continue to be a major crop for molecular farming and offers several practical advantages over...

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Detalles Bibliográficos
Autores principales: Tremblay, Reynald, Wang, David, Jevnikar, Anthony M., Ma, Shengwu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132750/
https://www.ncbi.nlm.nih.gov/pubmed/19961918
http://dx.doi.org/10.1016/j.biotechadv.2009.11.008
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author Tremblay, Reynald
Wang, David
Jevnikar, Anthony M.
Ma, Shengwu
author_facet Tremblay, Reynald
Wang, David
Jevnikar, Anthony M.
Ma, Shengwu
author_sort Tremblay, Reynald
collection PubMed
description Molecular farming of pharmaceuticals in plants has the potential to provide almost unlimited amounts of recombinant proteins for use in disease diagnosis, prevention or treatment. Tobacco has been and will continue to be a major crop for molecular farming and offers several practical advantages over other crops. It produces significant leaf biomass, has high soluble protein content and is a non-food crop, minimizing the risk of food-chain contamination. This, combined with its flexibility and highly-efficient genetic transformation/regeneration, has made tobacco particularly well suited for plant-based production of biopharmaceutical products. The goal of this review is to provide an update on the use of tobacco for molecular farming of biopharmaceuticals as well the technologies developed to enhance protein production/purification/efficacy. We show that tobacco is a robust biological reactor with a multitude of applications and may hold the key to success in plant molecular farming.
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spelling pubmed-71327502020-04-08 Tobacco, a highly efficient green bioreactor for production of therapeutic proteins Tremblay, Reynald Wang, David Jevnikar, Anthony M. Ma, Shengwu Biotechnol Adv Article Molecular farming of pharmaceuticals in plants has the potential to provide almost unlimited amounts of recombinant proteins for use in disease diagnosis, prevention or treatment. Tobacco has been and will continue to be a major crop for molecular farming and offers several practical advantages over other crops. It produces significant leaf biomass, has high soluble protein content and is a non-food crop, minimizing the risk of food-chain contamination. This, combined with its flexibility and highly-efficient genetic transformation/regeneration, has made tobacco particularly well suited for plant-based production of biopharmaceutical products. The goal of this review is to provide an update on the use of tobacco for molecular farming of biopharmaceuticals as well the technologies developed to enhance protein production/purification/efficacy. We show that tobacco is a robust biological reactor with a multitude of applications and may hold the key to success in plant molecular farming. Elsevier Inc. 2010 2009-12-02 /pmc/articles/PMC7132750/ /pubmed/19961918 http://dx.doi.org/10.1016/j.biotechadv.2009.11.008 Text en Copyright © 2009 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Tremblay, Reynald
Wang, David
Jevnikar, Anthony M.
Ma, Shengwu
Tobacco, a highly efficient green bioreactor for production of therapeutic proteins
title Tobacco, a highly efficient green bioreactor for production of therapeutic proteins
title_full Tobacco, a highly efficient green bioreactor for production of therapeutic proteins
title_fullStr Tobacco, a highly efficient green bioreactor for production of therapeutic proteins
title_full_unstemmed Tobacco, a highly efficient green bioreactor for production of therapeutic proteins
title_short Tobacco, a highly efficient green bioreactor for production of therapeutic proteins
title_sort tobacco, a highly efficient green bioreactor for production of therapeutic proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132750/
https://www.ncbi.nlm.nih.gov/pubmed/19961918
http://dx.doi.org/10.1016/j.biotechadv.2009.11.008
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