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FilmArray® respiratory panel performance in respiratory samples from neonatal care units

FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory...

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Autores principales: Piralla, Antonio, Lunghi, Giovanna, Percivalle, Elena, Viganò, Cristina, Nasta, Teresa, Pugni, Lorenza, Mosca, Fabio, Stronati, Mauro, Torresani, Erminio, Baldanti, Fausto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132758/
https://www.ncbi.nlm.nih.gov/pubmed/24666702
http://dx.doi.org/10.1016/j.diagmicrobio.2014.02.010
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author Piralla, Antonio
Lunghi, Giovanna
Percivalle, Elena
Viganò, Cristina
Nasta, Teresa
Pugni, Lorenza
Mosca, Fabio
Stronati, Mauro
Torresani, Erminio
Baldanti, Fausto
author_facet Piralla, Antonio
Lunghi, Giovanna
Percivalle, Elena
Viganò, Cristina
Nasta, Teresa
Pugni, Lorenza
Mosca, Fabio
Stronati, Mauro
Torresani, Erminio
Baldanti, Fausto
author_sort Piralla, Antonio
collection PubMed
description FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArray RP assay detected an additional virus (2 human rhinovirus/enterovirus and 2 bocavirus). In addition, FilmArray RP results for 70 of 80 (87.5%) respiratory samples tested were concordant with the Seegene Seeplex RV15® detection assay (Seegene, Inc., Seoul, South Korea), while 10/80 (12.5%) were discordant. The advantages of the FilmArray RP are the rapid detection of respiratory viruses (1 hour), the wide number of pathogens detectable in a single assay, and the reduced hands-on time.
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spelling pubmed-71327582020-04-08 FilmArray® respiratory panel performance in respiratory samples from neonatal care units Piralla, Antonio Lunghi, Giovanna Percivalle, Elena Viganò, Cristina Nasta, Teresa Pugni, Lorenza Mosca, Fabio Stronati, Mauro Torresani, Erminio Baldanti, Fausto Diagn Microbiol Infect Dis Article FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArray RP assay detected an additional virus (2 human rhinovirus/enterovirus and 2 bocavirus). In addition, FilmArray RP results for 70 of 80 (87.5%) respiratory samples tested were concordant with the Seegene Seeplex RV15® detection assay (Seegene, Inc., Seoul, South Korea), while 10/80 (12.5%) were discordant. The advantages of the FilmArray RP are the rapid detection of respiratory viruses (1 hour), the wide number of pathogens detectable in a single assay, and the reduced hands-on time. Elsevier Inc. 2014-06 2014-02-24 /pmc/articles/PMC7132758/ /pubmed/24666702 http://dx.doi.org/10.1016/j.diagmicrobio.2014.02.010 Text en Copyright © 2014 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Piralla, Antonio
Lunghi, Giovanna
Percivalle, Elena
Viganò, Cristina
Nasta, Teresa
Pugni, Lorenza
Mosca, Fabio
Stronati, Mauro
Torresani, Erminio
Baldanti, Fausto
FilmArray® respiratory panel performance in respiratory samples from neonatal care units
title FilmArray® respiratory panel performance in respiratory samples from neonatal care units
title_full FilmArray® respiratory panel performance in respiratory samples from neonatal care units
title_fullStr FilmArray® respiratory panel performance in respiratory samples from neonatal care units
title_full_unstemmed FilmArray® respiratory panel performance in respiratory samples from neonatal care units
title_short FilmArray® respiratory panel performance in respiratory samples from neonatal care units
title_sort filmarray® respiratory panel performance in respiratory samples from neonatal care units
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132758/
https://www.ncbi.nlm.nih.gov/pubmed/24666702
http://dx.doi.org/10.1016/j.diagmicrobio.2014.02.010
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