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Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats

BACKGROUND: The incidence of insulin resistance (IR) has rapidly increased worldwide over the last 20 years, no perfect solution has yet been identified. Finding new therapeutic drugs will help improve this situation. As a traditional Chinese medicine, PPM (processed Polygonum multiflorum) has widel...

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Autores principales: Gu, Wen, Yang, Min, Bi, Qian, Zeng, Lin-Xi, Wang, Xi, Dong, Jin-Cai, Li, Feng-Jiao, Yang, Xing-Xin, Li, Jing-Ping, Yu, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132990/
https://www.ncbi.nlm.nih.gov/pubmed/32248799
http://dx.doi.org/10.1186/s12906-020-02897-5
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author Gu, Wen
Yang, Min
Bi, Qian
Zeng, Lin-Xi
Wang, Xi
Dong, Jin-Cai
Li, Feng-Jiao
Yang, Xing-Xin
Li, Jing-Ping
Yu, Jie
author_facet Gu, Wen
Yang, Min
Bi, Qian
Zeng, Lin-Xi
Wang, Xi
Dong, Jin-Cai
Li, Feng-Jiao
Yang, Xing-Xin
Li, Jing-Ping
Yu, Jie
author_sort Gu, Wen
collection PubMed
description BACKGROUND: The incidence of insulin resistance (IR) has rapidly increased worldwide over the last 20 years, no perfect solution has yet been identified. Finding new therapeutic drugs will help improve this situation. As a traditional Chinese medicine, PPM (processed Polygonum multiflorum) has widely been used in the clinic. Recently, other clinical functions of PPM have been widely analyzed. RESULTS: Administration of the water extract from PPM decreased the level of FBG, TC, and TG, and increased the level of FGC, thereby reducing the IR index and improving IR. Furthermore, Western blot analysis revealed that PPM significantly increased GPR43 and AMPK expression when compared with the MOD group, and GPR43, AMPK were known as glucose metabolism-related proteins. In addition, treatment with PPM can restore the balance of gut microbiota by adjusting the relative abundance of bacteria both at the phylum and genus level, and these changes have been reported to be related to IR. METHODS: Sprague Dawley (SD) rats were fed a high-fat diet and were gavaged daily with either normal saline solution or PPM for 12 weeks. Major biochemical indexes, such as fasting blood glucose (FBG), fasting glucagon (FGC), total cholesterol (TC), and triglyceride (TG) were measured. Then the protein expression of adenosine 5′-monophosphate -activated protein kinase (AMPK) and G protein-coupled receptor 43 (GPR43) was evaluated by using Western blot analysis. Moreover, the composition of gut microbiota was assessed by analyzing 16S rRNA sequences. CONCLUSIONS: Our findings showed that PPM reversed the increasing of FBG and the decreasing of IRI, PPM accelerated the expression of glucose metabolism-related proteins and regulated the intestinal microecological balance. Therefore, we hold the opinion that PPM may be an effective option for treating IR.
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spelling pubmed-71329902020-04-11 Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats Gu, Wen Yang, Min Bi, Qian Zeng, Lin-Xi Wang, Xi Dong, Jin-Cai Li, Feng-Jiao Yang, Xing-Xin Li, Jing-Ping Yu, Jie BMC Complement Med Ther Research Article BACKGROUND: The incidence of insulin resistance (IR) has rapidly increased worldwide over the last 20 years, no perfect solution has yet been identified. Finding new therapeutic drugs will help improve this situation. As a traditional Chinese medicine, PPM (processed Polygonum multiflorum) has widely been used in the clinic. Recently, other clinical functions of PPM have been widely analyzed. RESULTS: Administration of the water extract from PPM decreased the level of FBG, TC, and TG, and increased the level of FGC, thereby reducing the IR index and improving IR. Furthermore, Western blot analysis revealed that PPM significantly increased GPR43 and AMPK expression when compared with the MOD group, and GPR43, AMPK were known as glucose metabolism-related proteins. In addition, treatment with PPM can restore the balance of gut microbiota by adjusting the relative abundance of bacteria both at the phylum and genus level, and these changes have been reported to be related to IR. METHODS: Sprague Dawley (SD) rats were fed a high-fat diet and were gavaged daily with either normal saline solution or PPM for 12 weeks. Major biochemical indexes, such as fasting blood glucose (FBG), fasting glucagon (FGC), total cholesterol (TC), and triglyceride (TG) were measured. Then the protein expression of adenosine 5′-monophosphate -activated protein kinase (AMPK) and G protein-coupled receptor 43 (GPR43) was evaluated by using Western blot analysis. Moreover, the composition of gut microbiota was assessed by analyzing 16S rRNA sequences. CONCLUSIONS: Our findings showed that PPM reversed the increasing of FBG and the decreasing of IRI, PPM accelerated the expression of glucose metabolism-related proteins and regulated the intestinal microecological balance. Therefore, we hold the opinion that PPM may be an effective option for treating IR. BioMed Central 2020-04-05 /pmc/articles/PMC7132990/ /pubmed/32248799 http://dx.doi.org/10.1186/s12906-020-02897-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Gu, Wen
Yang, Min
Bi, Qian
Zeng, Lin-Xi
Wang, Xi
Dong, Jin-Cai
Li, Feng-Jiao
Yang, Xing-Xin
Li, Jing-Ping
Yu, Jie
Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats
title Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats
title_full Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats
title_fullStr Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats
title_full_unstemmed Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats
title_short Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats
title_sort water extract from processed polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132990/
https://www.ncbi.nlm.nih.gov/pubmed/32248799
http://dx.doi.org/10.1186/s12906-020-02897-5
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