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Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography

Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which reli...

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Autores principales: Lee, Donghyeon, Park, Sehan, Lee, Keondo, Kim, Jangwoo, Park, Gisu, Nam, Ki Hyun, Baek, Sangwon, Chung, Wan Kyun, Lee, Jong-Lam, Cho, Yunje, Park, Jaehyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133064/
https://www.ncbi.nlm.nih.gov/pubmed/32280322
http://dx.doi.org/10.1107/S1600576720002423
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author Lee, Donghyeon
Park, Sehan
Lee, Keondo
Kim, Jangwoo
Park, Gisu
Nam, Ki Hyun
Baek, Sangwon
Chung, Wan Kyun
Lee, Jong-Lam
Cho, Yunje
Park, Jaehyun
author_facet Lee, Donghyeon
Park, Sehan
Lee, Keondo
Kim, Jangwoo
Park, Gisu
Nam, Ki Hyun
Baek, Sangwon
Chung, Wan Kyun
Lee, Jong-Lam
Cho, Yunje
Park, Jaehyun
author_sort Lee, Donghyeon
collection PubMed
description Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 Å resolution.
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spelling pubmed-71330642020-04-10 Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography Lee, Donghyeon Park, Sehan Lee, Keondo Kim, Jangwoo Park, Gisu Nam, Ki Hyun Baek, Sangwon Chung, Wan Kyun Lee, Jong-Lam Cho, Yunje Park, Jaehyun J Appl Crystallogr Research Papers Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 Å resolution. International Union of Crystallography 2020-03-25 /pmc/articles/PMC7133064/ /pubmed/32280322 http://dx.doi.org/10.1107/S1600576720002423 Text en © Donghyeon Lee et al. 2020 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Papers
Lee, Donghyeon
Park, Sehan
Lee, Keondo
Kim, Jangwoo
Park, Gisu
Nam, Ki Hyun
Baek, Sangwon
Chung, Wan Kyun
Lee, Jong-Lam
Cho, Yunje
Park, Jaehyun
Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography
title Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography
title_full Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography
title_fullStr Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography
title_full_unstemmed Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography
title_short Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography
title_sort application of a high-throughput microcrystal delivery system to serial femtosecond crystallography
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133064/
https://www.ncbi.nlm.nih.gov/pubmed/32280322
http://dx.doi.org/10.1107/S1600576720002423
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