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Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography
Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which reli...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133064/ https://www.ncbi.nlm.nih.gov/pubmed/32280322 http://dx.doi.org/10.1107/S1600576720002423 |
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author | Lee, Donghyeon Park, Sehan Lee, Keondo Kim, Jangwoo Park, Gisu Nam, Ki Hyun Baek, Sangwon Chung, Wan Kyun Lee, Jong-Lam Cho, Yunje Park, Jaehyun |
author_facet | Lee, Donghyeon Park, Sehan Lee, Keondo Kim, Jangwoo Park, Gisu Nam, Ki Hyun Baek, Sangwon Chung, Wan Kyun Lee, Jong-Lam Cho, Yunje Park, Jaehyun |
author_sort | Lee, Donghyeon |
collection | PubMed |
description | Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 Å resolution. |
format | Online Article Text |
id | pubmed-7133064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-71330642020-04-10 Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography Lee, Donghyeon Park, Sehan Lee, Keondo Kim, Jangwoo Park, Gisu Nam, Ki Hyun Baek, Sangwon Chung, Wan Kyun Lee, Jong-Lam Cho, Yunje Park, Jaehyun J Appl Crystallogr Research Papers Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 Å resolution. International Union of Crystallography 2020-03-25 /pmc/articles/PMC7133064/ /pubmed/32280322 http://dx.doi.org/10.1107/S1600576720002423 Text en © Donghyeon Lee et al. 2020 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Research Papers Lee, Donghyeon Park, Sehan Lee, Keondo Kim, Jangwoo Park, Gisu Nam, Ki Hyun Baek, Sangwon Chung, Wan Kyun Lee, Jong-Lam Cho, Yunje Park, Jaehyun Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography |
title | Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography |
title_full | Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography |
title_fullStr | Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography |
title_full_unstemmed | Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography |
title_short | Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography |
title_sort | application of a high-throughput microcrystal delivery system to serial femtosecond crystallography |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133064/ https://www.ncbi.nlm.nih.gov/pubmed/32280322 http://dx.doi.org/10.1107/S1600576720002423 |
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