A trans-spliced leader sequence on actin mRNA in C. elegans

While determining the 5′ ends of C. elegans actin mRNAs, we have discovered a 22 nucleotide spliced leader sequence. The leader sequence is found on mRNA from three of the four nematode actin genes. The leader also appears to be present on some, but not all, nonactin mRNAs. The actin mRNA leader seq...

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Detalles Bibliográficos
Autores principales: Krause, Michael, Hirsh, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 1987
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133374/
https://www.ncbi.nlm.nih.gov/pubmed/3581169
http://dx.doi.org/10.1016/0092-8674(87)90613-1
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author Krause, Michael
Hirsh, David
author_facet Krause, Michael
Hirsh, David
author_sort Krause, Michael
collection PubMed
description While determining the 5′ ends of C. elegans actin mRNAs, we have discovered a 22 nucleotide spliced leader sequence. The leader sequence is found on mRNA from three of the four nematode actin genes. The leader also appears to be present on some, but not all, nonactin mRNAs. The actin mRNA leader sequence is identical to the first 22 nucleotides of a novel 100 nucleotide RNA transcribed adjacent, and in the opposite orientation, to the 5S ribosomal gene. The evidence suggests that the actin mRNA leader sequence is acquired from this novel nucleotide transcript by an intermolecular trans-splicing mechanism.
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spelling pubmed-71333742020-04-08 A trans-spliced leader sequence on actin mRNA in C. elegans Krause, Michael Hirsh, David Cell Article While determining the 5′ ends of C. elegans actin mRNAs, we have discovered a 22 nucleotide spliced leader sequence. The leader sequence is found on mRNA from three of the four nematode actin genes. The leader also appears to be present on some, but not all, nonactin mRNAs. The actin mRNA leader sequence is identical to the first 22 nucleotides of a novel 100 nucleotide RNA transcribed adjacent, and in the opposite orientation, to the 5S ribosomal gene. The evidence suggests that the actin mRNA leader sequence is acquired from this novel nucleotide transcript by an intermolecular trans-splicing mechanism. Cell Press 1987-06-19 2004-04-16 /pmc/articles/PMC7133374/ /pubmed/3581169 http://dx.doi.org/10.1016/0092-8674(87)90613-1 Text en Copyright © 1987 . Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Krause, Michael
Hirsh, David
A trans-spliced leader sequence on actin mRNA in C. elegans
title A trans-spliced leader sequence on actin mRNA in C. elegans
title_full A trans-spliced leader sequence on actin mRNA in C. elegans
title_fullStr A trans-spliced leader sequence on actin mRNA in C. elegans
title_full_unstemmed A trans-spliced leader sequence on actin mRNA in C. elegans
title_short A trans-spliced leader sequence on actin mRNA in C. elegans
title_sort trans-spliced leader sequence on actin mrna in c. elegans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133374/
https://www.ncbi.nlm.nih.gov/pubmed/3581169
http://dx.doi.org/10.1016/0092-8674(87)90613-1
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