Quantitative determination of lymphocyte ACTH(1–39)

A method was developed for quantitative measurement of ACTH(1–39), produced by human lymphocytes. It was shown that pH adjustment to 2 was essential for processing of the precursor molecule proopiomelanocortin (POMC). Linearity between time of incubation and ACTH production was shown. The existence...

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Detalles Bibliográficos
Autores principales: Ferreira, J.A., Carstens, M.E., Taljaard, J.J.F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Ltd. 1990
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133553/
https://www.ncbi.nlm.nih.gov/pubmed/2158005
http://dx.doi.org/10.1016/0143-4179(90)90154-Q
Descripción
Sumario:A method was developed for quantitative measurement of ACTH(1–39), produced by human lymphocytes. It was shown that pH adjustment to 2 was essential for processing of the precursor molecule proopiomelanocortin (POMC). Linearity between time of incubation and ACTH production was shown. The existence of specific endopeptidases in lymphocytes for processing of the POMC molecule remains doubtful.

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