Quantitative determination of lymphocyte ACTH(1–39)

A method was developed for quantitative measurement of ACTH(1–39), produced by human lymphocytes. It was shown that pH adjustment to 2 was essential for processing of the precursor molecule proopiomelanocortin (POMC). Linearity between time of incubation and ACTH production was shown. The existence...

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Detalles Bibliográficos
Autores principales: Ferreira, J.A., Carstens, M.E., Taljaard, J.J.F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Ltd. 1990
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133553/
https://www.ncbi.nlm.nih.gov/pubmed/2158005
http://dx.doi.org/10.1016/0143-4179(90)90154-Q
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author Ferreira, J.A.
Carstens, M.E.
Taljaard, J.J.F.
author_facet Ferreira, J.A.
Carstens, M.E.
Taljaard, J.J.F.
author_sort Ferreira, J.A.
collection PubMed
description A method was developed for quantitative measurement of ACTH(1–39), produced by human lymphocytes. It was shown that pH adjustment to 2 was essential for processing of the precursor molecule proopiomelanocortin (POMC). Linearity between time of incubation and ACTH production was shown. The existence of specific endopeptidases in lymphocytes for processing of the POMC molecule remains doubtful.
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spelling pubmed-71335532020-04-08 Quantitative determination of lymphocyte ACTH(1–39) Ferreira, J.A. Carstens, M.E. Taljaard, J.J.F. Neuropeptides Article A method was developed for quantitative measurement of ACTH(1–39), produced by human lymphocytes. It was shown that pH adjustment to 2 was essential for processing of the precursor molecule proopiomelanocortin (POMC). Linearity between time of incubation and ACTH production was shown. The existence of specific endopeptidases in lymphocytes for processing of the POMC molecule remains doubtful. Published by Elsevier Ltd. 1990-01 2004-05-13 /pmc/articles/PMC7133553/ /pubmed/2158005 http://dx.doi.org/10.1016/0143-4179(90)90154-Q Text en Copyright © 1990 Published by Elsevier Ltd. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Ferreira, J.A.
Carstens, M.E.
Taljaard, J.J.F.
Quantitative determination of lymphocyte ACTH(1–39)
title Quantitative determination of lymphocyte ACTH(1–39)
title_full Quantitative determination of lymphocyte ACTH(1–39)
title_fullStr Quantitative determination of lymphocyte ACTH(1–39)
title_full_unstemmed Quantitative determination of lymphocyte ACTH(1–39)
title_short Quantitative determination of lymphocyte ACTH(1–39)
title_sort quantitative determination of lymphocyte acth(1–39)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133553/
https://www.ncbi.nlm.nih.gov/pubmed/2158005
http://dx.doi.org/10.1016/0143-4179(90)90154-Q
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