Antiviral and Antigenic Properties of Recombinant Porcine Interferon Gamma

Recombinant porcine interferon gamma (rPoIFNγ) induced a dose-dependent inhibition of the cytopathic effect produced by vesicular stomatitis virus (VSV) challenge of both homologous and heterologous (bovine) cell lines. In addition, an antiviral effect of rPoIFNγ was demonstrable against the coronav...

Descripción completa

Detalles Bibliográficos
Autores principales: Charley, B., McCullough, K., Martinod, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1988
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133658/
https://www.ncbi.nlm.nih.gov/pubmed/2847405
http://dx.doi.org/10.1016/0165-2427(88)90001-3
_version_ 1783517670472155136
author Charley, B.
McCullough, K.
Martinod, S.
author_facet Charley, B.
McCullough, K.
Martinod, S.
author_sort Charley, B.
collection PubMed
description Recombinant porcine interferon gamma (rPoIFNγ) induced a dose-dependent inhibition of the cytopathic effect produced by vesicular stomatitis virus (VSV) challenge of both homologous and heterologous (bovine) cell lines. In addition, an antiviral effect of rPoIFNγ was demonstrable against the coronavirus transmissible gastroenteritis virus (TGEV) infection of porcine epithelial cells and of pulmonary macrophages. A rabbit anti-PoIFNγ antiserum was prepared and shown to specifically neutralize the antiviral effects of natural and recombinant porcine IFNγ preparations. This antiserum could also neutralize recombinant bovine IFNγ but not recombinant human IFNγ. These results suggest antigenic homology of porcine and bovine IFNγ but antigenic differences between these molecules and human IFNγ.
format Online
Article
Text
id pubmed-7133658
institution National Center for Biotechnology Information
language English
publishDate 1988
publisher Published by Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-71336582020-04-08 Antiviral and Antigenic Properties of Recombinant Porcine Interferon Gamma Charley, B. McCullough, K. Martinod, S. Vet Immunol Immunopathol Article Recombinant porcine interferon gamma (rPoIFNγ) induced a dose-dependent inhibition of the cytopathic effect produced by vesicular stomatitis virus (VSV) challenge of both homologous and heterologous (bovine) cell lines. In addition, an antiviral effect of rPoIFNγ was demonstrable against the coronavirus transmissible gastroenteritis virus (TGEV) infection of porcine epithelial cells and of pulmonary macrophages. A rabbit anti-PoIFNγ antiserum was prepared and shown to specifically neutralize the antiviral effects of natural and recombinant porcine IFNγ preparations. This antiserum could also neutralize recombinant bovine IFNγ but not recombinant human IFNγ. These results suggest antigenic homology of porcine and bovine IFNγ but antigenic differences between these molecules and human IFNγ. Published by Elsevier B.V. 1988-09 2002-11-13 /pmc/articles/PMC7133658/ /pubmed/2847405 http://dx.doi.org/10.1016/0165-2427(88)90001-3 Text en Copyright © 1988 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Charley, B.
McCullough, K.
Martinod, S.
Antiviral and Antigenic Properties of Recombinant Porcine Interferon Gamma
title Antiviral and Antigenic Properties of Recombinant Porcine Interferon Gamma
title_full Antiviral and Antigenic Properties of Recombinant Porcine Interferon Gamma
title_fullStr Antiviral and Antigenic Properties of Recombinant Porcine Interferon Gamma
title_full_unstemmed Antiviral and Antigenic Properties of Recombinant Porcine Interferon Gamma
title_short Antiviral and Antigenic Properties of Recombinant Porcine Interferon Gamma
title_sort antiviral and antigenic properties of recombinant porcine interferon gamma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133658/
https://www.ncbi.nlm.nih.gov/pubmed/2847405
http://dx.doi.org/10.1016/0165-2427(88)90001-3
work_keys_str_mv AT charleyb antiviralandantigenicpropertiesofrecombinantporcineinterferongamma
AT mcculloughk antiviralandantigenicpropertiesofrecombinantporcineinterferongamma
AT martinods antiviralandantigenicpropertiesofrecombinantporcineinterferongamma

Ejemplares similares