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A Bovine Monoclonal Antibody to Oestrone/Oestradiol Prepared by a (Murine×Bovine) ×Bovine Interspecies Fusion

A heterohybridoma was produced by the fusion of sensitized peripheral blood lymphocytes (PBLs) with a previously derived heteromyeloma, generated by the fusion of bovine PBLs with murine myeloma cells. The sensitized bovine PBLs were collected from a steer immunized with an oestradiol-ovalbumin conj...

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Detalles Bibliográficos
Autores principales: Groves, D.J., Clayton, J., Morris, B.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1988
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133659/
https://www.ncbi.nlm.nih.gov/pubmed/3376424
http://dx.doi.org/10.1016/0165-2427(88)90039-6
Descripción
Sumario:A heterohybridoma was produced by the fusion of sensitized peripheral blood lymphocytes (PBLs) with a previously derived heteromyeloma, generated by the fusion of bovine PBLs with murine myeloma cells. The sensitized bovine PBLs were collected from a steer immunized with an oestradiol-ovalbumin conjugate. The cell lines resulting from the fusion were screened for the production of bovine antibodies to oestradiol. A stable heterohybridoma was isolated which secreted a bovine IgG(1) to oestrone/oestradiol. The use of sensitized PBLs together with heteromyeloma fusion partners has proved to be a reliable and simple way of producing monoclonal antibodies against specific haptens.