A Bovine Monoclonal Antibody to Oestrone/Oestradiol Prepared by a (Murine×Bovine) ×Bovine Interspecies Fusion

A heterohybridoma was produced by the fusion of sensitized peripheral blood lymphocytes (PBLs) with a previously derived heteromyeloma, generated by the fusion of bovine PBLs with murine myeloma cells. The sensitized bovine PBLs were collected from a steer immunized with an oestradiol-ovalbumin conj...

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Detalles Bibliográficos
Autores principales: Groves, D.J., Clayton, J., Morris, B.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1988
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133659/
https://www.ncbi.nlm.nih.gov/pubmed/3376424
http://dx.doi.org/10.1016/0165-2427(88)90039-6
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author Groves, D.J.
Clayton, J.
Morris, B.A.
author_facet Groves, D.J.
Clayton, J.
Morris, B.A.
author_sort Groves, D.J.
collection PubMed
description A heterohybridoma was produced by the fusion of sensitized peripheral blood lymphocytes (PBLs) with a previously derived heteromyeloma, generated by the fusion of bovine PBLs with murine myeloma cells. The sensitized bovine PBLs were collected from a steer immunized with an oestradiol-ovalbumin conjugate. The cell lines resulting from the fusion were screened for the production of bovine antibodies to oestradiol. A stable heterohybridoma was isolated which secreted a bovine IgG(1) to oestrone/oestradiol. The use of sensitized PBLs together with heteromyeloma fusion partners has proved to be a reliable and simple way of producing monoclonal antibodies against specific haptens.
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spelling pubmed-71336592020-04-08 A Bovine Monoclonal Antibody to Oestrone/Oestradiol Prepared by a (Murine×Bovine) ×Bovine Interspecies Fusion Groves, D.J. Clayton, J. Morris, B.A. Vet Immunol Immunopathol Article A heterohybridoma was produced by the fusion of sensitized peripheral blood lymphocytes (PBLs) with a previously derived heteromyeloma, generated by the fusion of bovine PBLs with murine myeloma cells. The sensitized bovine PBLs were collected from a steer immunized with an oestradiol-ovalbumin conjugate. The cell lines resulting from the fusion were screened for the production of bovine antibodies to oestradiol. A stable heterohybridoma was isolated which secreted a bovine IgG(1) to oestrone/oestradiol. The use of sensitized PBLs together with heteromyeloma fusion partners has proved to be a reliable and simple way of producing monoclonal antibodies against specific haptens. Published by Elsevier B.V. 1988-02 2002-11-11 /pmc/articles/PMC7133659/ /pubmed/3376424 http://dx.doi.org/10.1016/0165-2427(88)90039-6 Text en Copyright © 1988 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Groves, D.J.
Clayton, J.
Morris, B.A.
A Bovine Monoclonal Antibody to Oestrone/Oestradiol Prepared by a (Murine×Bovine) ×Bovine Interspecies Fusion
title A Bovine Monoclonal Antibody to Oestrone/Oestradiol Prepared by a (Murine×Bovine) ×Bovine Interspecies Fusion
title_full A Bovine Monoclonal Antibody to Oestrone/Oestradiol Prepared by a (Murine×Bovine) ×Bovine Interspecies Fusion
title_fullStr A Bovine Monoclonal Antibody to Oestrone/Oestradiol Prepared by a (Murine×Bovine) ×Bovine Interspecies Fusion
title_full_unstemmed A Bovine Monoclonal Antibody to Oestrone/Oestradiol Prepared by a (Murine×Bovine) ×Bovine Interspecies Fusion
title_short A Bovine Monoclonal Antibody to Oestrone/Oestradiol Prepared by a (Murine×Bovine) ×Bovine Interspecies Fusion
title_sort bovine monoclonal antibody to oestrone/oestradiol prepared by a (murine×bovine) ×bovine interspecies fusion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133659/
https://www.ncbi.nlm.nih.gov/pubmed/3376424
http://dx.doi.org/10.1016/0165-2427(88)90039-6
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