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Persistence of Viruses in Upper Respiratory Tract of Children with Asthma

Objectives: Nasopharyngeal swabs of 50 asthmatic children in the symptom-free period were examined for the presence of adenoviruses, rhinoviruses and coronaviruses. A control group of 20 healthy individuals was included in this study. Methods: A polymerase chain reaction was used to detect adenoviru...

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Autores principales: Marin, J, Jeler-Kačar, D, Levstek, V, Maček, V
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The British Infection Society. Published by Elsevier Ltd. 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133682/
https://www.ncbi.nlm.nih.gov/pubmed/10942643
http://dx.doi.org/10.1053/jinf.2000.0688
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author Marin, J
Jeler-Kačar, D
Levstek, V
Maček, V
author_facet Marin, J
Jeler-Kačar, D
Levstek, V
Maček, V
author_sort Marin, J
collection PubMed
description Objectives: Nasopharyngeal swabs of 50 asthmatic children in the symptom-free period were examined for the presence of adenoviruses, rhinoviruses and coronaviruses. A control group of 20 healthy individuals was included in this study. Methods: A polymerase chain reaction was used to detect adenovirus DNA and rhinovirus and coronavirus complementary DNA. The fragments of amplified genetic material were visualized with the use of agarose gel electrophoresis. Results: Adenovirus DNA was found in 78.4% of asthmatic children, rhinovirus RNA in 32.4% and coronavirus RNA in 2.7%. Adenovirus DNA was detected in one of the 20 nasopharyngeal swabs of healthy controls; the rest of the control samples were negative. Conclusions: The persistent presence of viruses in the upper respiratory tract of asthmatic children shows a possible connection between viral infections and asthma.
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spelling pubmed-71336822020-04-08 Persistence of Viruses in Upper Respiratory Tract of Children with Asthma Marin, J Jeler-Kačar, D Levstek, V Maček, V J Infect Regular Article Objectives: Nasopharyngeal swabs of 50 asthmatic children in the symptom-free period were examined for the presence of adenoviruses, rhinoviruses and coronaviruses. A control group of 20 healthy individuals was included in this study. Methods: A polymerase chain reaction was used to detect adenovirus DNA and rhinovirus and coronavirus complementary DNA. The fragments of amplified genetic material were visualized with the use of agarose gel electrophoresis. Results: Adenovirus DNA was found in 78.4% of asthmatic children, rhinovirus RNA in 32.4% and coronavirus RNA in 2.7%. Adenovirus DNA was detected in one of the 20 nasopharyngeal swabs of healthy controls; the rest of the control samples were negative. Conclusions: The persistent presence of viruses in the upper respiratory tract of asthmatic children shows a possible connection between viral infections and asthma. The British Infection Society. Published by Elsevier Ltd. 2000-07 2002-05-25 /pmc/articles/PMC7133682/ /pubmed/10942643 http://dx.doi.org/10.1053/jinf.2000.0688 Text en Copyright © 2000 The British Infection Society. Published by Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Regular Article
Marin, J
Jeler-Kačar, D
Levstek, V
Maček, V
Persistence of Viruses in Upper Respiratory Tract of Children with Asthma
title Persistence of Viruses in Upper Respiratory Tract of Children with Asthma
title_full Persistence of Viruses in Upper Respiratory Tract of Children with Asthma
title_fullStr Persistence of Viruses in Upper Respiratory Tract of Children with Asthma
title_full_unstemmed Persistence of Viruses in Upper Respiratory Tract of Children with Asthma
title_short Persistence of Viruses in Upper Respiratory Tract of Children with Asthma
title_sort persistence of viruses in upper respiratory tract of children with asthma
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133682/
https://www.ncbi.nlm.nih.gov/pubmed/10942643
http://dx.doi.org/10.1053/jinf.2000.0688
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