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Proportional morbidity rates of enteropathogens among diarrheic dairy calves in central Spain
Faecal samples from 218 diarrheic dairy calves in 65 dairy herds, selected by convenience, were screened for the presence of rotavirus, coronavirus, Cryptosporidium spp., F5(+)Escherichia coli and Salmonella spp. Animals surveyed were from 1 to 30 days old. Cryptosporidium and rotavirus were the mos...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science B.V.
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133840/ https://www.ncbi.nlm.nih.gov/pubmed/9762735 http://dx.doi.org/10.1016/S0167-5877(98)00077-4 |
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author | de la Fuente, R García, A Ruiz-Santa-Quiteria, J.A Luzón, M Cid, D García, S Orden, J.A Gómez-Bautista, M |
author_facet | de la Fuente, R García, A Ruiz-Santa-Quiteria, J.A Luzón, M Cid, D García, S Orden, J.A Gómez-Bautista, M |
author_sort | de la Fuente, R |
collection | PubMed |
description | Faecal samples from 218 diarrheic dairy calves in 65 dairy herds, selected by convenience, were screened for the presence of rotavirus, coronavirus, Cryptosporidium spp., F5(+)Escherichia coli and Salmonella spp. Animals surveyed were from 1 to 30 days old. Cryptosporidium and rotavirus were the most commonly detected agents (52.3% and 42.7% of the samples positive, respectively). F5(+)E. coli was detected in the faeces of 11.9% of the calves and bovine coronavirus was detected in the faeces of 7.3% of the calves. Salmonella spp. was only found in the faeces of two calves (0.9%). Mixed infections with two or more agents occurred in 28% of the calves. Concurrent infection of rotavirus and Cryptosporidium was found in 21.6% of the calves. Two tests were used for the detection of rotavirus (a commercial ELISA and PAGE), F5(+)E. coli (ELISA and bacterial culture) and Cryptosporidium (ELISA and microscopy). The validity of the commercial ELISA for the detection of rotavirus, F5(+)E. coli and Cryptosporidium in faeces from diarrheic calves was evaluated using PAGE, bacterial culture and microscopy as gold standard, respectively. The ELISA showed a very low sensitivity (28.6%) for the detection of F5(+)E. coli compared to bacterial culture. |
format | Online Article Text |
id | pubmed-7133840 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1998 |
publisher | Elsevier Science B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71338402020-04-08 Proportional morbidity rates of enteropathogens among diarrheic dairy calves in central Spain de la Fuente, R García, A Ruiz-Santa-Quiteria, J.A Luzón, M Cid, D García, S Orden, J.A Gómez-Bautista, M Prev Vet Med Article Faecal samples from 218 diarrheic dairy calves in 65 dairy herds, selected by convenience, were screened for the presence of rotavirus, coronavirus, Cryptosporidium spp., F5(+)Escherichia coli and Salmonella spp. Animals surveyed were from 1 to 30 days old. Cryptosporidium and rotavirus were the most commonly detected agents (52.3% and 42.7% of the samples positive, respectively). F5(+)E. coli was detected in the faeces of 11.9% of the calves and bovine coronavirus was detected in the faeces of 7.3% of the calves. Salmonella spp. was only found in the faeces of two calves (0.9%). Mixed infections with two or more agents occurred in 28% of the calves. Concurrent infection of rotavirus and Cryptosporidium was found in 21.6% of the calves. Two tests were used for the detection of rotavirus (a commercial ELISA and PAGE), F5(+)E. coli (ELISA and bacterial culture) and Cryptosporidium (ELISA and microscopy). The validity of the commercial ELISA for the detection of rotavirus, F5(+)E. coli and Cryptosporidium in faeces from diarrheic calves was evaluated using PAGE, bacterial culture and microscopy as gold standard, respectively. The ELISA showed a very low sensitivity (28.6%) for the detection of F5(+)E. coli compared to bacterial culture. Elsevier Science B.V. 1998-08-07 1998-08-10 /pmc/articles/PMC7133840/ /pubmed/9762735 http://dx.doi.org/10.1016/S0167-5877(98)00077-4 Text en Copyright © 1998 Elsevier Science B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article de la Fuente, R García, A Ruiz-Santa-Quiteria, J.A Luzón, M Cid, D García, S Orden, J.A Gómez-Bautista, M Proportional morbidity rates of enteropathogens among diarrheic dairy calves in central Spain |
title | Proportional morbidity rates of enteropathogens among diarrheic dairy calves in central Spain |
title_full | Proportional morbidity rates of enteropathogens among diarrheic dairy calves in central Spain |
title_fullStr | Proportional morbidity rates of enteropathogens among diarrheic dairy calves in central Spain |
title_full_unstemmed | Proportional morbidity rates of enteropathogens among diarrheic dairy calves in central Spain |
title_short | Proportional morbidity rates of enteropathogens among diarrheic dairy calves in central Spain |
title_sort | proportional morbidity rates of enteropathogens among diarrheic dairy calves in central spain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133840/ https://www.ncbi.nlm.nih.gov/pubmed/9762735 http://dx.doi.org/10.1016/S0167-5877(98)00077-4 |
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