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Effect of different routes of immunization with bovine rotavirus on lactogenic antibody response in mice()

The effect of different routes of immunization with either live or killed bovine rotavirus (BRV) on the production of lactogenic antibody response in mice was evaluated. The routes of immunization were intramuscular (IM), oral (O) or intradermal in the mammary region (IMam). Following immunization,...

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Autores principales: Ijaz, M.K., Sabara, M.I., Frenchick, P.J., Babiuk, L.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1987
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133845/
https://www.ncbi.nlm.nih.gov/pubmed/2837143
http://dx.doi.org/10.1016/S0166-3542(87)80006-2
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author Ijaz, M.K.
Sabara, M.I.
Frenchick, P.J.
Babiuk, L.A.
author_facet Ijaz, M.K.
Sabara, M.I.
Frenchick, P.J.
Babiuk, L.A.
author_sort Ijaz, M.K.
collection PubMed
description The effect of different routes of immunization with either live or killed bovine rotavirus (BRV) on the production of lactogenic antibody response in mice was evaluated. The routes of immunization were intramuscular (IM), oral (O) or intradermal in the mammary region (IMam). Following immunization, serum antibody responses were monitored by an enzyme linked immunosorbent assay (ELISA). Following whelping, the mice were allowed to stay with their mother until sacrificed on alternate days post-parturition from day 1–11. Milk from their stomach was collected for antibody titration by ELISA and virus neutralization test. Regardless of the routes of immunization, a rapid increase in serum anti-rotavirus antibody titers was observed for the first 5 wk after immunization followed by a gradual decline. After parturition, the mean antibody titer of lacteal secretions, as determined by ELISA, increased gradually for 7 days with the greatest increase on day 9, followed by a decrease in anti-rotavirus antibody. These titers also correlated with antibody titers in milk as measured by virus neutralization test. The best lactogenic antibody response was observed when IMam × IM × 2 route of immunization was used with live BRV as the antigen. Interestingly, immunization via the oral route with killed BRV also resulted in good antibody responses. In contrast, in the group where killed BRV was used, animals receiving 3× orally had the highest antibody titer. The distribution of different antibody subtypes in milk samples revealed IgG to be the predominant antibody followed by IgM and IgA. Irrespective of the route of administration, there was an increase in IgA on day 9 as compared to day 1 in most of the groups. The significant role played by mucosal immunity in passive protection and the possible ways to modulate subtype specific lactogenic immune response are discussed. Animals models; Lactogenic immunity; Rotaviruses
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spelling pubmed-71338452020-04-08 Effect of different routes of immunization with bovine rotavirus on lactogenic antibody response in mice() Ijaz, M.K. Sabara, M.I. Frenchick, P.J. Babiuk, L.A. Antiviral Res Article The effect of different routes of immunization with either live or killed bovine rotavirus (BRV) on the production of lactogenic antibody response in mice was evaluated. The routes of immunization were intramuscular (IM), oral (O) or intradermal in the mammary region (IMam). Following immunization, serum antibody responses were monitored by an enzyme linked immunosorbent assay (ELISA). Following whelping, the mice were allowed to stay with their mother until sacrificed on alternate days post-parturition from day 1–11. Milk from their stomach was collected for antibody titration by ELISA and virus neutralization test. Regardless of the routes of immunization, a rapid increase in serum anti-rotavirus antibody titers was observed for the first 5 wk after immunization followed by a gradual decline. After parturition, the mean antibody titer of lacteal secretions, as determined by ELISA, increased gradually for 7 days with the greatest increase on day 9, followed by a decrease in anti-rotavirus antibody. These titers also correlated with antibody titers in milk as measured by virus neutralization test. The best lactogenic antibody response was observed when IMam × IM × 2 route of immunization was used with live BRV as the antigen. Interestingly, immunization via the oral route with killed BRV also resulted in good antibody responses. In contrast, in the group where killed BRV was used, animals receiving 3× orally had the highest antibody titer. The distribution of different antibody subtypes in milk samples revealed IgG to be the predominant antibody followed by IgM and IgA. Irrespective of the route of administration, there was an increase in IgA on day 9 as compared to day 1 in most of the groups. The significant role played by mucosal immunity in passive protection and the possible ways to modulate subtype specific lactogenic immune response are discussed. Animals models; Lactogenic immunity; Rotaviruses Published by Elsevier B.V. 1987-12 2004-11-30 /pmc/articles/PMC7133845/ /pubmed/2837143 http://dx.doi.org/10.1016/S0166-3542(87)80006-2 Text en Copyright © 1987 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Ijaz, M.K.
Sabara, M.I.
Frenchick, P.J.
Babiuk, L.A.
Effect of different routes of immunization with bovine rotavirus on lactogenic antibody response in mice()
title Effect of different routes of immunization with bovine rotavirus on lactogenic antibody response in mice()
title_full Effect of different routes of immunization with bovine rotavirus on lactogenic antibody response in mice()
title_fullStr Effect of different routes of immunization with bovine rotavirus on lactogenic antibody response in mice()
title_full_unstemmed Effect of different routes of immunization with bovine rotavirus on lactogenic antibody response in mice()
title_short Effect of different routes of immunization with bovine rotavirus on lactogenic antibody response in mice()
title_sort effect of different routes of immunization with bovine rotavirus on lactogenic antibody response in mice()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133845/
https://www.ncbi.nlm.nih.gov/pubmed/2837143
http://dx.doi.org/10.1016/S0166-3542(87)80006-2
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