Localization of major neutralizing epitopes on the S1 polypeptide of the murine coronavirus peplomer glycoprotein

A recombinant baculovirus system has been used to express the amino terminal half of the murine coronavirus (JHMV) peplomer glycoprotein in insect cells. The expressed polypeptide is glycosylated and is recognized by a set of monoclonal antibodies (mAbs) specific for JHMV S protein. Three of these m...

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Detalles Bibliográficos
Autores principales: Sayaka, Takase-Yoden, Tateki, Kikuchi, Siddell, Stuart G., Fumihiro, Taguchi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1991
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133859/
https://www.ncbi.nlm.nih.gov/pubmed/1645909
http://dx.doi.org/10.1016/0168-1702(91)90011-J
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author Sayaka, Takase-Yoden
Tateki, Kikuchi
Siddell, Stuart G.
Fumihiro, Taguchi
author_facet Sayaka, Takase-Yoden
Tateki, Kikuchi
Siddell, Stuart G.
Fumihiro, Taguchi
author_sort Sayaka, Takase-Yoden
collection PubMed
description A recombinant baculovirus system has been used to express the amino terminal half of the murine coronavirus (JHMV) peplomer glycoprotein in insect cells. The expressed polypeptide is glycosylated and is recognized by a set of monoclonal antibodies (mAbs) specific for JHMV S protein. Three of these mAbs have a very high neutralizing activity for JHMV but not for other MHV strains. These results indicate that JHMV-specific, major neutralizing epitopes reside in the amino terminal S1 subunit of the peplomer glycoprotein.
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spelling pubmed-71338592020-04-08 Localization of major neutralizing epitopes on the S1 polypeptide of the murine coronavirus peplomer glycoprotein Sayaka, Takase-Yoden Tateki, Kikuchi Siddell, Stuart G. Fumihiro, Taguchi Virus Res Article A recombinant baculovirus system has been used to express the amino terminal half of the murine coronavirus (JHMV) peplomer glycoprotein in insect cells. The expressed polypeptide is glycosylated and is recognized by a set of monoclonal antibodies (mAbs) specific for JHMV S protein. Three of these mAbs have a very high neutralizing activity for JHMV but not for other MHV strains. These results indicate that JHMV-specific, major neutralizing epitopes reside in the amino terminal S1 subunit of the peplomer glycoprotein. Published by Elsevier B.V. 1991-03 2002-11-12 /pmc/articles/PMC7133859/ /pubmed/1645909 http://dx.doi.org/10.1016/0168-1702(91)90011-J Text en Copyright © 1991 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Sayaka, Takase-Yoden
Tateki, Kikuchi
Siddell, Stuart G.
Fumihiro, Taguchi
Localization of major neutralizing epitopes on the S1 polypeptide of the murine coronavirus peplomer glycoprotein
title Localization of major neutralizing epitopes on the S1 polypeptide of the murine coronavirus peplomer glycoprotein
title_full Localization of major neutralizing epitopes on the S1 polypeptide of the murine coronavirus peplomer glycoprotein
title_fullStr Localization of major neutralizing epitopes on the S1 polypeptide of the murine coronavirus peplomer glycoprotein
title_full_unstemmed Localization of major neutralizing epitopes on the S1 polypeptide of the murine coronavirus peplomer glycoprotein
title_short Localization of major neutralizing epitopes on the S1 polypeptide of the murine coronavirus peplomer glycoprotein
title_sort localization of major neutralizing epitopes on the s1 polypeptide of the murine coronavirus peplomer glycoprotein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133859/
https://www.ncbi.nlm.nih.gov/pubmed/1645909
http://dx.doi.org/10.1016/0168-1702(91)90011-J
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