In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of Rabbit Hemorrhagic Disease Virus (RHDV)

Purified preparations of the Spanish field isolate of rabbit hemorrhagic disease virus AST/89 were found to contain the plus-stranded genomic RNA of more than 7.4 kilobases (kb) and large amounts of a subgenomic mRNA of 2.4 kb. The smaller RNA was translated in vitro and shown to code for a 60 kDa p...

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Autores principales: Boga, JoséAntonio, Marín, María Soledad, Casais, Rosa, Prieto, Miguel, Parra, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1992
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133906/
https://www.ncbi.nlm.nih.gov/pubmed/1441736
http://dx.doi.org/10.1016/0168-1702(92)90144-X
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author Boga, JoséAntonio
Marín, María Soledad
Casais, Rosa
Prieto, Miguel
Parra, Francisco
author_facet Boga, JoséAntonio
Marín, María Soledad
Casais, Rosa
Prieto, Miguel
Parra, Francisco
author_sort Boga, JoséAntonio
collection PubMed
description Purified preparations of the Spanish field isolate of rabbit hemorrhagic disease virus AST/89 were found to contain the plus-stranded genomic RNA of more than 7.4 kilobases (kb) and large amounts of a subgenomic mRNA of 2.4 kb. The smaller RNA was translated in vitro and shown to code for a 60 kDa protein which was immunoprecipitated using anti-RHDV as well as anti-VP60 sera.
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spelling pubmed-71339062020-04-08 In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of Rabbit Hemorrhagic Disease Virus (RHDV) Boga, JoséAntonio Marín, María Soledad Casais, Rosa Prieto, Miguel Parra, Francisco Virus Res Article Purified preparations of the Spanish field isolate of rabbit hemorrhagic disease virus AST/89 were found to contain the plus-stranded genomic RNA of more than 7.4 kilobases (kb) and large amounts of a subgenomic mRNA of 2.4 kb. The smaller RNA was translated in vitro and shown to code for a 60 kDa protein which was immunoprecipitated using anti-RHDV as well as anti-VP60 sera. Published by Elsevier B.V. 1992-10 2002-11-12 /pmc/articles/PMC7133906/ /pubmed/1441736 http://dx.doi.org/10.1016/0168-1702(92)90144-X Text en Copyright © 1992 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Boga, JoséAntonio
Marín, María Soledad
Casais, Rosa
Prieto, Miguel
Parra, Francisco
In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of Rabbit Hemorrhagic Disease Virus (RHDV)
title In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of Rabbit Hemorrhagic Disease Virus (RHDV)
title_full In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of Rabbit Hemorrhagic Disease Virus (RHDV)
title_fullStr In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of Rabbit Hemorrhagic Disease Virus (RHDV)
title_full_unstemmed In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of Rabbit Hemorrhagic Disease Virus (RHDV)
title_short In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of Rabbit Hemorrhagic Disease Virus (RHDV)
title_sort in vitro translation of a subgenomic mrna from purified virions of the spanish field isolate ast/89 of rabbit hemorrhagic disease virus (rhdv)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133906/
https://www.ncbi.nlm.nih.gov/pubmed/1441736
http://dx.doi.org/10.1016/0168-1702(92)90144-X
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