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Bovine coronavirus hemagglutinin protein
Treatment of purified bovine coronavirus (Mebus strain) with pronase destroyed the integrity of virion surface glycoproteins gp140, gp120, gp100, reduced the amount of gp26 and destroyed the hemagglutinating activity of the virus. Bromelain, on the other hand, destroyed the integrity of gp120, gp100...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1985
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133962/ https://www.ncbi.nlm.nih.gov/pubmed/3984492 http://dx.doi.org/10.1016/0168-1702(85)90059-0 |
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author | King, Bernadette Potts, Barbara J. Brian, David A. |
author_facet | King, Bernadette Potts, Barbara J. Brian, David A. |
author_sort | King, Bernadette |
collection | PubMed |
description | Treatment of purified bovine coronavirus (Mebus strain) with pronase destroyed the integrity of virion surface glycoproteins gp140, gp120, gp100, reduced the amount of gp26 and destroyed the hemagglutinating activity of the virus. Bromelain, on the other hand, destroyed the integrity of gp120, gp100 and gp26 but failed to remove gp140 and failed to destroy viral hemagglutinating activity. These experiments suggest that gp140 is the virion hemagglutinin. Immunoblotting studies using monospecific antiserum demonstrate that gp140 is a disulfide-linked dimeric structure reducible to monomers of 65 kDa. |
format | Online Article Text |
id | pubmed-7133962 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1985 |
publisher | Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71339622020-04-08 Bovine coronavirus hemagglutinin protein King, Bernadette Potts, Barbara J. Brian, David A. Virus Res Article Treatment of purified bovine coronavirus (Mebus strain) with pronase destroyed the integrity of virion surface glycoproteins gp140, gp120, gp100, reduced the amount of gp26 and destroyed the hemagglutinating activity of the virus. Bromelain, on the other hand, destroyed the integrity of gp120, gp100 and gp26 but failed to remove gp140 and failed to destroy viral hemagglutinating activity. These experiments suggest that gp140 is the virion hemagglutinin. Immunoblotting studies using monospecific antiserum demonstrate that gp140 is a disulfide-linked dimeric structure reducible to monomers of 65 kDa. Published by Elsevier B.V. 1985-02 2002-11-12 /pmc/articles/PMC7133962/ /pubmed/3984492 http://dx.doi.org/10.1016/0168-1702(85)90059-0 Text en Copyright © 1985 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article King, Bernadette Potts, Barbara J. Brian, David A. Bovine coronavirus hemagglutinin protein |
title | Bovine coronavirus hemagglutinin protein |
title_full | Bovine coronavirus hemagglutinin protein |
title_fullStr | Bovine coronavirus hemagglutinin protein |
title_full_unstemmed | Bovine coronavirus hemagglutinin protein |
title_short | Bovine coronavirus hemagglutinin protein |
title_sort | bovine coronavirus hemagglutinin protein |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7133962/ https://www.ncbi.nlm.nih.gov/pubmed/3984492 http://dx.doi.org/10.1016/0168-1702(85)90059-0 |
work_keys_str_mv | AT kingbernadette bovinecoronavirushemagglutininprotein AT pottsbarbaraj bovinecoronavirushemagglutininprotein AT briandavida bovinecoronavirushemagglutininprotein |