Alphavirus replication in cultured cells and infected animals is inhibited by antiproteinase agents

The influence of different antiproteinase agents on alphavirus replication was examined. Sindbis virus multicycle replication in cultured cells was suppressed by N-tosyl-phenylalanine chloromethyl ketone (TPCK), an inhibitor of chymotrypsin-like proteinases, and by aprotinin, an inhibitor of a wide...

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Detalles Bibliográficos
Autores principales: Zhirnov, O.P., Ovcharenko, A.V., Melnikova, E.E., Bukrinskaya, A.G., Gaidamovich, S.Ya.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1986
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7134154/
https://www.ncbi.nlm.nih.gov/pubmed/2429615
http://dx.doi.org/10.1016/0166-3542(86)90021-5
Descripción
Sumario:The influence of different antiproteinase agents on alphavirus replication was examined. Sindbis virus multicycle replication in cultured cells was suppressed by N-tosyl-phenylalanine chloromethyl ketone (TPCK), an inhibitor of chymotrypsin-like proteinases, and by aprotinin, an inhibitor of a wide spectrum of proteinases. Antiviral activity of TPCK was also demonstrated in Sindbis virus-infected animals. Parenteral injections of TPCK in infected mice reduced virus titers in brain and blood. The possible mechanism(s) of antiviral action of the antiproteinase agents are discussed.

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