Alphavirus replication in cultured cells and infected animals is inhibited by antiproteinase agents

The influence of different antiproteinase agents on alphavirus replication was examined. Sindbis virus multicycle replication in cultured cells was suppressed by N-tosyl-phenylalanine chloromethyl ketone (TPCK), an inhibitor of chymotrypsin-like proteinases, and by aprotinin, an inhibitor of a wide...

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Detalles Bibliográficos
Autores principales: Zhirnov, O.P., Ovcharenko, A.V., Melnikova, E.E., Bukrinskaya, A.G., Gaidamovich, S.Ya.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1986
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7134154/
https://www.ncbi.nlm.nih.gov/pubmed/2429615
http://dx.doi.org/10.1016/0166-3542(86)90021-5
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author Zhirnov, O.P.
Ovcharenko, A.V.
Melnikova, E.E.
Bukrinskaya, A.G.
Gaidamovich, S.Ya.
author_facet Zhirnov, O.P.
Ovcharenko, A.V.
Melnikova, E.E.
Bukrinskaya, A.G.
Gaidamovich, S.Ya.
author_sort Zhirnov, O.P.
collection PubMed
description The influence of different antiproteinase agents on alphavirus replication was examined. Sindbis virus multicycle replication in cultured cells was suppressed by N-tosyl-phenylalanine chloromethyl ketone (TPCK), an inhibitor of chymotrypsin-like proteinases, and by aprotinin, an inhibitor of a wide spectrum of proteinases. Antiviral activity of TPCK was also demonstrated in Sindbis virus-infected animals. Parenteral injections of TPCK in infected mice reduced virus titers in brain and blood. The possible mechanism(s) of antiviral action of the antiproteinase agents are discussed.
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spelling pubmed-71341542020-04-08 Alphavirus replication in cultured cells and infected animals is inhibited by antiproteinase agents Zhirnov, O.P. Ovcharenko, A.V. Melnikova, E.E. Bukrinskaya, A.G. Gaidamovich, S.Ya. Antiviral Res Article The influence of different antiproteinase agents on alphavirus replication was examined. Sindbis virus multicycle replication in cultured cells was suppressed by N-tosyl-phenylalanine chloromethyl ketone (TPCK), an inhibitor of chymotrypsin-like proteinases, and by aprotinin, an inhibitor of a wide spectrum of proteinases. Antiviral activity of TPCK was also demonstrated in Sindbis virus-infected animals. Parenteral injections of TPCK in infected mice reduced virus titers in brain and blood. The possible mechanism(s) of antiviral action of the antiproteinase agents are discussed. Published by Elsevier B.V. 1986-08 2002-11-12 /pmc/articles/PMC7134154/ /pubmed/2429615 http://dx.doi.org/10.1016/0166-3542(86)90021-5 Text en Copyright © 1986 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zhirnov, O.P.
Ovcharenko, A.V.
Melnikova, E.E.
Bukrinskaya, A.G.
Gaidamovich, S.Ya.
Alphavirus replication in cultured cells and infected animals is inhibited by antiproteinase agents
title Alphavirus replication in cultured cells and infected animals is inhibited by antiproteinase agents
title_full Alphavirus replication in cultured cells and infected animals is inhibited by antiproteinase agents
title_fullStr Alphavirus replication in cultured cells and infected animals is inhibited by antiproteinase agents
title_full_unstemmed Alphavirus replication in cultured cells and infected animals is inhibited by antiproteinase agents
title_short Alphavirus replication in cultured cells and infected animals is inhibited by antiproteinase agents
title_sort alphavirus replication in cultured cells and infected animals is inhibited by antiproteinase agents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7134154/
https://www.ncbi.nlm.nih.gov/pubmed/2429615
http://dx.doi.org/10.1016/0166-3542(86)90021-5
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