Analysis and localisation of mouse hepatitis virus 3 (MHV3) polypeptides

The analysis by SDS-polyacrylamide gel electrophoresis of purified mouse hepatitis virus type 3 revealed the presence of five polypeptides, three of them contained carbohydrate. The molecular weight of these proteins was 190,000 daltons (VGP(100)), 100,000 (VGP(100)), 58,000 (VP(58)), 25,000 (VP(25)...

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Autores principales: Obert, G., Grollemund, E., Nonnenmacher, H., Kirn, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Masson SAS 1981
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7134784/
http://dx.doi.org/10.1016/S0769-2617(81)80059-7
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author Obert, G.
Grollemund, E.
Nonnenmacher, H.
Kirn, A.
author_facet Obert, G.
Grollemund, E.
Nonnenmacher, H.
Kirn, A.
author_sort Obert, G.
collection PubMed
description The analysis by SDS-polyacrylamide gel electrophoresis of purified mouse hepatitis virus type 3 revealed the presence of five polypeptides, three of them contained carbohydrate. The molecular weight of these proteins was 190,000 daltons (VGP(100)), 100,000 (VGP(100)), 58,000 (VP(58)), 25,000 (VP(25)) and 24,000 (VGP(24)). The analysis of particles obtained after limited proteolysis with bromelain suggests that VGP(190) and VGP(100) form the spike layer, that VP(25) and VGP(24) are membrane-associated and that VP(58) represents the inner core polypeptide.
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spelling pubmed-71347842020-04-08 Analysis and localisation of mouse hepatitis virus 3 (MHV3) polypeptides Obert, G. Grollemund, E. Nonnenmacher, H. Kirn, A. Ann Inst Pasteur Virol Article The analysis by SDS-polyacrylamide gel electrophoresis of purified mouse hepatitis virus type 3 revealed the presence of five polypeptides, three of them contained carbohydrate. The molecular weight of these proteins was 190,000 daltons (VGP(100)), 100,000 (VGP(100)), 58,000 (VP(58)), 25,000 (VP(25)) and 24,000 (VGP(24)). The analysis of particles obtained after limited proteolysis with bromelain suggests that VGP(190) and VGP(100) form the spike layer, that VP(25) and VGP(24) are membrane-associated and that VP(58) represents the inner core polypeptide. Published by Elsevier Masson SAS 1981 2006-11-03 /pmc/articles/PMC7134784/ http://dx.doi.org/10.1016/S0769-2617(81)80059-7 Text en Copyright © 1981 Published by Elsevier Masson SAS. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Obert, G.
Grollemund, E.
Nonnenmacher, H.
Kirn, A.
Analysis and localisation of mouse hepatitis virus 3 (MHV3) polypeptides
title Analysis and localisation of mouse hepatitis virus 3 (MHV3) polypeptides
title_full Analysis and localisation of mouse hepatitis virus 3 (MHV3) polypeptides
title_fullStr Analysis and localisation of mouse hepatitis virus 3 (MHV3) polypeptides
title_full_unstemmed Analysis and localisation of mouse hepatitis virus 3 (MHV3) polypeptides
title_short Analysis and localisation of mouse hepatitis virus 3 (MHV3) polypeptides
title_sort analysis and localisation of mouse hepatitis virus 3 (mhv3) polypeptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7134784/
http://dx.doi.org/10.1016/S0769-2617(81)80059-7
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