Evaluation of nested polymerase chain methods for the detection of human coronaviruses 229E and OC43

Currently, the diagnosis of human respiratory coronavirus infection is either slow or insensitive. This paper describes nested polymerase chain reaction assays for the detection of human coronaviruses OC43 and 229E. The specificity and sensitivity of the assays have been determined and they have bee...

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Detalles Bibliográficos
Autores principales: Myint, S., Johnston, S., Sanderson, G., Simpson, H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press. 1994
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7135046/
https://www.ncbi.nlm.nih.gov/pubmed/7877631
http://dx.doi.org/10.1006/mcpr.1994.1052
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author Myint, S.
Johnston, S.
Sanderson, G.
Simpson, H.
author_facet Myint, S.
Johnston, S.
Sanderson, G.
Simpson, H.
author_sort Myint, S.
collection PubMed
description Currently, the diagnosis of human respiratory coronavirus infection is either slow or insensitive. This paper describes nested polymerase chain reaction assays for the detection of human coronaviruses OC43 and 229E. The specificity and sensitivity of the assays have been determined and they have been applied to the detection of the viruses in nasal aspirates. These assays are more rapid and sensitive than cell culture and may replace the latter as the diagnostic method of choice.
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spelling pubmed-71350462020-04-08 Evaluation of nested polymerase chain methods for the detection of human coronaviruses 229E and OC43 Myint, S. Johnston, S. Sanderson, G. Simpson, H. Mol Cell Probes Original Articles Currently, the diagnosis of human respiratory coronavirus infection is either slow or insensitive. This paper describes nested polymerase chain reaction assays for the detection of human coronaviruses OC43 and 229E. The specificity and sensitivity of the assays have been determined and they have been applied to the detection of the viruses in nasal aspirates. These assays are more rapid and sensitive than cell culture and may replace the latter as the diagnostic method of choice. Academic Press. 1994-10 2002-05-25 /pmc/articles/PMC7135046/ /pubmed/7877631 http://dx.doi.org/10.1006/mcpr.1994.1052 Text en Copyright © 1994 Academic Press. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Original Articles
Myint, S.
Johnston, S.
Sanderson, G.
Simpson, H.
Evaluation of nested polymerase chain methods for the detection of human coronaviruses 229E and OC43
title Evaluation of nested polymerase chain methods for the detection of human coronaviruses 229E and OC43
title_full Evaluation of nested polymerase chain methods for the detection of human coronaviruses 229E and OC43
title_fullStr Evaluation of nested polymerase chain methods for the detection of human coronaviruses 229E and OC43
title_full_unstemmed Evaluation of nested polymerase chain methods for the detection of human coronaviruses 229E and OC43
title_short Evaluation of nested polymerase chain methods for the detection of human coronaviruses 229E and OC43
title_sort evaluation of nested polymerase chain methods for the detection of human coronaviruses 229e and oc43
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7135046/
https://www.ncbi.nlm.nih.gov/pubmed/7877631
http://dx.doi.org/10.1006/mcpr.1994.1052
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