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Biotinylated and radioactive cDNA probes in the detection by hybridization of bovine enteric coronavirus
cDNA, synthesized on bovine coronavirus (BCV) genomic RNA templates, could be used to detect very small quantities (i.e. 1 pg) of viral RNA by hybridization with either radioisotopic-labelled or biotinylated recombinant plasmids. Virus was optimally attached to nitrocellulose membranes when spotted...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Published by Elsevier Ltd.
1988
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7135618/ https://www.ncbi.nlm.nih.gov/pubmed/3221884 http://dx.doi.org/10.1016/0890-8508(88)90005-9 |