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Synthesis of long cDNA from viral RNA template()
Methods to make long and reliable cDNA from viral RNA template have been optimized. The conditions of the denaturation of the viral RNA template were most critical. For synthesis of the first DNA strand, the concentration of the primer and the presence of an RNase inhibitor were important. During th...
Autores principales: | Lenstra, J.A., De Groot, R.J., Jacobs, L., Kusters, J.G., Niesters, H.G.M., Van Der Zeijst, B.A.M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1988
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7135641/ https://www.ncbi.nlm.nih.gov/pubmed/3192156 http://dx.doi.org/10.1016/0735-0651(88)90017-9 |
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