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Structural morphing in a symmetry-mismatched viral vertex
Large biological structures are assembled from smaller, often symmetric, sub-structures. However, asymmetry among sub-structures is fundamentally important for biological function. An extreme form of asymmetry, a 12-fold-symmetric dodecameric portal complex inserted into a 5-fold-symmetric capsid ve...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136217/ https://www.ncbi.nlm.nih.gov/pubmed/32249784 http://dx.doi.org/10.1038/s41467-020-15575-4 |
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author | Fang, Qianglin Tang, Wei-Chun Tao, Pan Mahalingam, Marthandan Fokine, Andrei Rossmann, Michael G. Rao, Venigalla B. |
author_facet | Fang, Qianglin Tang, Wei-Chun Tao, Pan Mahalingam, Marthandan Fokine, Andrei Rossmann, Michael G. Rao, Venigalla B. |
author_sort | Fang, Qianglin |
collection | PubMed |
description | Large biological structures are assembled from smaller, often symmetric, sub-structures. However, asymmetry among sub-structures is fundamentally important for biological function. An extreme form of asymmetry, a 12-fold-symmetric dodecameric portal complex inserted into a 5-fold-symmetric capsid vertex, is found in numerous icosahedral viruses, including tailed bacteriophages, herpesviruses, and archaeal viruses. This vertex is critical for driving capsid assembly, DNA packaging, tail attachment, and genome ejection. Here, we report the near-atomic in situ structure of the symmetry-mismatched portal vertex from bacteriophage T4. Remarkably, the local structure of portal morphs to compensate for symmetry-mismatch, forming similar interactions in different capsid environments while maintaining strict symmetry in the rest of the structure. This creates a unique and unusually dynamic symmetry-mismatched vertex that is central to building an infectious virion. |
format | Online Article Text |
id | pubmed-7136217 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-71362172020-04-08 Structural morphing in a symmetry-mismatched viral vertex Fang, Qianglin Tang, Wei-Chun Tao, Pan Mahalingam, Marthandan Fokine, Andrei Rossmann, Michael G. Rao, Venigalla B. Nat Commun Article Large biological structures are assembled from smaller, often symmetric, sub-structures. However, asymmetry among sub-structures is fundamentally important for biological function. An extreme form of asymmetry, a 12-fold-symmetric dodecameric portal complex inserted into a 5-fold-symmetric capsid vertex, is found in numerous icosahedral viruses, including tailed bacteriophages, herpesviruses, and archaeal viruses. This vertex is critical for driving capsid assembly, DNA packaging, tail attachment, and genome ejection. Here, we report the near-atomic in situ structure of the symmetry-mismatched portal vertex from bacteriophage T4. Remarkably, the local structure of portal morphs to compensate for symmetry-mismatch, forming similar interactions in different capsid environments while maintaining strict symmetry in the rest of the structure. This creates a unique and unusually dynamic symmetry-mismatched vertex that is central to building an infectious virion. Nature Publishing Group UK 2020-04-06 /pmc/articles/PMC7136217/ /pubmed/32249784 http://dx.doi.org/10.1038/s41467-020-15575-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Fang, Qianglin Tang, Wei-Chun Tao, Pan Mahalingam, Marthandan Fokine, Andrei Rossmann, Michael G. Rao, Venigalla B. Structural morphing in a symmetry-mismatched viral vertex |
title | Structural morphing in a symmetry-mismatched viral vertex |
title_full | Structural morphing in a symmetry-mismatched viral vertex |
title_fullStr | Structural morphing in a symmetry-mismatched viral vertex |
title_full_unstemmed | Structural morphing in a symmetry-mismatched viral vertex |
title_short | Structural morphing in a symmetry-mismatched viral vertex |
title_sort | structural morphing in a symmetry-mismatched viral vertex |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136217/ https://www.ncbi.nlm.nih.gov/pubmed/32249784 http://dx.doi.org/10.1038/s41467-020-15575-4 |
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