Cargando…
The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties
Oxidative stress and inflammation play a key role in the age-related decline in the respiratory function. Adipokine in relation to the metabolic and inflammatory systems is attracting growing interest in the field of respiratory dysfunction. The present clinical and experimental studies investigated...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136289/ https://www.ncbi.nlm.nih.gov/pubmed/32249844 http://dx.doi.org/10.1038/s41598-020-62872-5 |
_version_ | 1783518216116502528 |
---|---|
author | Oniki, Kentaro Nohara, Hirofumi Nakashima, Ryunosuke Obata, Yui Muto, Narumi Sakamoto, Yuki Ueno-Shuto, Keiko Imafuku, Tadashi Ishima, Yu Watanabe, Hiroshi Maruyama, Toru Otake, Koji Ogata, Yasuhiro Suico, Mary Ann Kai, Hirofumi Shuto, Tsuyoshi Saruwatari, Junji |
author_facet | Oniki, Kentaro Nohara, Hirofumi Nakashima, Ryunosuke Obata, Yui Muto, Narumi Sakamoto, Yuki Ueno-Shuto, Keiko Imafuku, Tadashi Ishima, Yu Watanabe, Hiroshi Maruyama, Toru Otake, Koji Ogata, Yasuhiro Suico, Mary Ann Kai, Hirofumi Shuto, Tsuyoshi Saruwatari, Junji |
author_sort | Oniki, Kentaro |
collection | PubMed |
description | Oxidative stress and inflammation play a key role in the age-related decline in the respiratory function. Adipokine in relation to the metabolic and inflammatory systems is attracting growing interest in the field of respiratory dysfunction. The present clinical and experimental studies investigated the role of the disulfide bond-forming oxidoreductase A-like protein (DsbA-L) gene, which has antioxidant and adiponectin multimeric (i.e. activation) properties, on the respiratory function of the elderly. We performed a retrospective longitudinal genotype-phenotype relationship analysis of 318 Japanese relatively elderly participants (mean age ± standard deviation: 67.0 ± 5.8 years) during a health screening program and an in vitro DsbA-L knock-down evaluation using 16HBE14o-cells, a commonly evaluated human airway epithelial cell line. The DsbA-L rs1917760 polymorphism was associated with a reduction in the ratio of forced expiratory volume in 1 second (FEV1)/forced vital capacity (FVC) and %FEV1 and with the elevation of the prevalence of FEV1/FVC < 70%. We also confirmed that the polymorphism was associated with a decreased respiratory function in relation to a decrease in the ratio of high-molecular-weight adiponectin/total adiponectin (as a marker of adiponectin multimerization) and an increase in the oxidized human serum albumin (as an oxidative stress marker). Furthermore, we clarified that DsbA-L knock-down induced oxidative stress and up-regulated the mucus production in human airway epithelial cells. These findings suggest that the DsbA-L gene may play a role in protecting the respiratory function of the elderly, possibly via increased systemic adiponectin functions secreted from adipocytes or through systemic and/or local pulmonary antioxidant properties. |
format | Online Article Text |
id | pubmed-7136289 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-71362892020-04-11 The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties Oniki, Kentaro Nohara, Hirofumi Nakashima, Ryunosuke Obata, Yui Muto, Narumi Sakamoto, Yuki Ueno-Shuto, Keiko Imafuku, Tadashi Ishima, Yu Watanabe, Hiroshi Maruyama, Toru Otake, Koji Ogata, Yasuhiro Suico, Mary Ann Kai, Hirofumi Shuto, Tsuyoshi Saruwatari, Junji Sci Rep Article Oxidative stress and inflammation play a key role in the age-related decline in the respiratory function. Adipokine in relation to the metabolic and inflammatory systems is attracting growing interest in the field of respiratory dysfunction. The present clinical and experimental studies investigated the role of the disulfide bond-forming oxidoreductase A-like protein (DsbA-L) gene, which has antioxidant and adiponectin multimeric (i.e. activation) properties, on the respiratory function of the elderly. We performed a retrospective longitudinal genotype-phenotype relationship analysis of 318 Japanese relatively elderly participants (mean age ± standard deviation: 67.0 ± 5.8 years) during a health screening program and an in vitro DsbA-L knock-down evaluation using 16HBE14o-cells, a commonly evaluated human airway epithelial cell line. The DsbA-L rs1917760 polymorphism was associated with a reduction in the ratio of forced expiratory volume in 1 second (FEV1)/forced vital capacity (FVC) and %FEV1 and with the elevation of the prevalence of FEV1/FVC < 70%. We also confirmed that the polymorphism was associated with a decreased respiratory function in relation to a decrease in the ratio of high-molecular-weight adiponectin/total adiponectin (as a marker of adiponectin multimerization) and an increase in the oxidized human serum albumin (as an oxidative stress marker). Furthermore, we clarified that DsbA-L knock-down induced oxidative stress and up-regulated the mucus production in human airway epithelial cells. These findings suggest that the DsbA-L gene may play a role in protecting the respiratory function of the elderly, possibly via increased systemic adiponectin functions secreted from adipocytes or through systemic and/or local pulmonary antioxidant properties. Nature Publishing Group UK 2020-04-06 /pmc/articles/PMC7136289/ /pubmed/32249844 http://dx.doi.org/10.1038/s41598-020-62872-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Oniki, Kentaro Nohara, Hirofumi Nakashima, Ryunosuke Obata, Yui Muto, Narumi Sakamoto, Yuki Ueno-Shuto, Keiko Imafuku, Tadashi Ishima, Yu Watanabe, Hiroshi Maruyama, Toru Otake, Koji Ogata, Yasuhiro Suico, Mary Ann Kai, Hirofumi Shuto, Tsuyoshi Saruwatari, Junji The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties |
title | The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties |
title_full | The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties |
title_fullStr | The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties |
title_full_unstemmed | The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties |
title_short | The DsbA-L gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties |
title_sort | dsba-l gene is associated with respiratory function of the elderly via its adiponectin multimeric or antioxidant properties |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136289/ https://www.ncbi.nlm.nih.gov/pubmed/32249844 http://dx.doi.org/10.1038/s41598-020-62872-5 |
work_keys_str_mv | AT onikikentaro thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT noharahirofumi thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT nakashimaryunosuke thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT obatayui thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT mutonarumi thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT sakamotoyuki thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT uenoshutokeiko thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT imafukutadashi thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT ishimayu thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT watanabehiroshi thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT maruyamatoru thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT otakekoji thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT ogatayasuhiro thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT suicomaryann thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT kaihirofumi thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT shutotsuyoshi thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT saruwatarijunji thedsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT onikikentaro dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT noharahirofumi dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT nakashimaryunosuke dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT obatayui dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT mutonarumi dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT sakamotoyuki dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT uenoshutokeiko dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT imafukutadashi dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT ishimayu dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT watanabehiroshi dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT maruyamatoru dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT otakekoji dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT ogatayasuhiro dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT suicomaryann dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT kaihirofumi dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT shutotsuyoshi dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties AT saruwatarijunji dsbalgeneisassociatedwithrespiratoryfunctionoftheelderlyviaitsadiponectinmultimericorantioxidantproperties |