Cargando…

Intravesical Loss of OnabotulinumtoxinA During Endoscopic Intradetrusor Injection - A Multicenter Experience

PURPOSE: To evaluate intravesical loss of onabolunumtoxinA (onaBTA) during endoscopic injection into the bladder wall for treatment of neurogenic detrusor overactivity (NDO). The intraluminal loss of onaBTA cannot be determined directly, therefore we added methylene blue (MB) to reconstitute onaBTA....

Descripción completa

Detalles Bibliográficos
Autores principales: Šámal, Vladimír, Krhut, Jan, Paldus, Vít, Műllerová, Jana, Mečl, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Continence Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136441/
https://www.ncbi.nlm.nih.gov/pubmed/32252187
http://dx.doi.org/10.5213/inj.1938160.080
_version_ 1783518249708683264
author Šámal, Vladimír
Krhut, Jan
Paldus, Vít
Műllerová, Jana
Mečl, Jan
author_facet Šámal, Vladimír
Krhut, Jan
Paldus, Vít
Műllerová, Jana
Mečl, Jan
author_sort Šámal, Vladimír
collection PubMed
description PURPOSE: To evaluate intravesical loss of onabolunumtoxinA (onaBTA) during endoscopic injection into the bladder wall for treatment of neurogenic detrusor overactivity (NDO). The intraluminal loss of onaBTA cannot be determined directly, therefore we added methylene blue (MB) to reconstitute onaBTA. Subsequently we used spectrophotometry to determine the total amount of MB in the irrigation fluid, which allowed us to calculate total intraluminal loss of onaBTA. METHODS: The study population was consisted of 48 patients with NDO. The mean age was 36.6±8.9 years. Forty-two patients suffered from NDO due to spinal cord injury and 6 patients suffered from multiple sclerosis. Each patient received 200 units of onaBTA administered by 30 endoscopic injections (1 mL per injection) using a 23-G needle. The entire volume of irrigation fluid was collected and spectrophotometry was used to determine the MB concentration. The total amount of injected onaBTA, total amount of irrigation solution and the known amount of MB used during reconstitution, allowed for the determination of intravesical loss of onaBTA. RESULTS: Forty-five patients were included in the final analysis. The mean volume of irrigation fluid was 603.33±400.14 mL. The mean absorbance was 0.14±0.12 with the mean MB concentration 0.19±0.18 mg/L. The mean calculated loss of onaBTA was 4.14±4.11 units. CONCLUSIONS: The endoscopic injection of onaBTA marked by MB into the bladder wall is associated with minimal intravesical loss of the agent, representing less than 3% of the administered dose of MB. This may reflect the amount of the onaBTA detected in the irrigation fluid.
format Online
Article
Text
id pubmed-7136441
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Korean Continence Society
record_format MEDLINE/PubMed
spelling pubmed-71364412020-04-09 Intravesical Loss of OnabotulinumtoxinA During Endoscopic Intradetrusor Injection - A Multicenter Experience Šámal, Vladimír Krhut, Jan Paldus, Vít Műllerová, Jana Mečl, Jan Int Neurourol J Original Article PURPOSE: To evaluate intravesical loss of onabolunumtoxinA (onaBTA) during endoscopic injection into the bladder wall for treatment of neurogenic detrusor overactivity (NDO). The intraluminal loss of onaBTA cannot be determined directly, therefore we added methylene blue (MB) to reconstitute onaBTA. Subsequently we used spectrophotometry to determine the total amount of MB in the irrigation fluid, which allowed us to calculate total intraluminal loss of onaBTA. METHODS: The study population was consisted of 48 patients with NDO. The mean age was 36.6±8.9 years. Forty-two patients suffered from NDO due to spinal cord injury and 6 patients suffered from multiple sclerosis. Each patient received 200 units of onaBTA administered by 30 endoscopic injections (1 mL per injection) using a 23-G needle. The entire volume of irrigation fluid was collected and spectrophotometry was used to determine the MB concentration. The total amount of injected onaBTA, total amount of irrigation solution and the known amount of MB used during reconstitution, allowed for the determination of intravesical loss of onaBTA. RESULTS: Forty-five patients were included in the final analysis. The mean volume of irrigation fluid was 603.33±400.14 mL. The mean absorbance was 0.14±0.12 with the mean MB concentration 0.19±0.18 mg/L. The mean calculated loss of onaBTA was 4.14±4.11 units. CONCLUSIONS: The endoscopic injection of onaBTA marked by MB into the bladder wall is associated with minimal intravesical loss of the agent, representing less than 3% of the administered dose of MB. This may reflect the amount of the onaBTA detected in the irrigation fluid. Korean Continence Society 2020-03 2020-03-31 /pmc/articles/PMC7136441/ /pubmed/32252187 http://dx.doi.org/10.5213/inj.1938160.080 Text en Copyright © 2020 Korean Continence Society This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Šámal, Vladimír
Krhut, Jan
Paldus, Vít
Műllerová, Jana
Mečl, Jan
Intravesical Loss of OnabotulinumtoxinA During Endoscopic Intradetrusor Injection - A Multicenter Experience
title Intravesical Loss of OnabotulinumtoxinA During Endoscopic Intradetrusor Injection - A Multicenter Experience
title_full Intravesical Loss of OnabotulinumtoxinA During Endoscopic Intradetrusor Injection - A Multicenter Experience
title_fullStr Intravesical Loss of OnabotulinumtoxinA During Endoscopic Intradetrusor Injection - A Multicenter Experience
title_full_unstemmed Intravesical Loss of OnabotulinumtoxinA During Endoscopic Intradetrusor Injection - A Multicenter Experience
title_short Intravesical Loss of OnabotulinumtoxinA During Endoscopic Intradetrusor Injection - A Multicenter Experience
title_sort intravesical loss of onabotulinumtoxina during endoscopic intradetrusor injection - a multicenter experience
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136441/
https://www.ncbi.nlm.nih.gov/pubmed/32252187
http://dx.doi.org/10.5213/inj.1938160.080
work_keys_str_mv AT samalvladimir intravesicallossofonabotulinumtoxinaduringendoscopicintradetrusorinjectionamulticenterexperience
AT krhutjan intravesicallossofonabotulinumtoxinaduringendoscopicintradetrusorinjectionamulticenterexperience
AT paldusvit intravesicallossofonabotulinumtoxinaduringendoscopicintradetrusorinjectionamulticenterexperience
AT mullerovajana intravesicallossofonabotulinumtoxinaduringendoscopicintradetrusorinjectionamulticenterexperience
AT mecljan intravesicallossofonabotulinumtoxinaduringendoscopicintradetrusorinjectionamulticenterexperience