Cargando…

A new technical approach for preparing frozen biological samples for electron microscopy

BACKGROUND: Many methodological approaches have focused so far on physiological and molecular responses of plant tissues to freezing but only little knowledge is available on the consequences of extracellular ice-formation on cellular ultrastructure that underlies physiological reactions. In this co...

Descripción completa

Detalles Bibliográficos
Autores principales:	Buchner, Othmar, Steiner, Philip, Andosch, Ancuela, Holzinger, Andreas, Stegner, Matthias, Neuner, Gilbert, Lütz-Meindl, Ursula
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137184/ https://www.ncbi.nlm.nih.gov/pubmed/32280364 http://dx.doi.org/10.1186/s13007-020-00586-5

Ejemplares similares

Winter survival of the unicellular green alga Micrasterias denticulata: insights from field monitoring and simulation experiments
por: Steiner, Philip, et al.
Publicado: (2021)

Fusion of Mitochondria to 3-D Networks, Autophagy and Increased Organelle Contacts are Important Subcellular Hallmarks during Cold Stress in Plants
por: Steiner, Philip, et al.
Publicado: (2020)

Ice accommodation in plant tissues pinpointed by cryo-microscopy in reflected-polarised-light
por: Stegner, Matthias, et al.
Publicado: (2020)

Frozen mountain pine needles: The endodermis discriminates between the ice‐containing central tissue and the ice‐free fully functional mesophyll
por: Stegner, Matthias, et al.
Publicado: (2023)

Subcellular Sequestration and Impact of Heavy Metals on the Ultrastructure and Physiology of the Multicellular Freshwater Alga Desmidium swartzii
por: Andosch, Ancuela, et al.
Publicado: (2015)

Correction: Subcellular Sequestration and Impact of Heavy Metals on the Ultrastructure and Physiology of the Multicellular Freshwater Alga Desmidium swartzii
por: Andosch, Ancuela, et al.
Publicado: (2015)

Salt stress-induced cell death in the unicellular green alga Micrasterias denticulata
por: Affenzeller, Matthias Josef, et al.
Publicado: (2009)

A novel system for in situ determination of heat tolerance of plants: first results on alpine dwarf shrubs
por: Buchner, Othmar, et al.
Publicado: (2013)

Cell Wall Reinforcements Accompany Chilling and Freezing Stress in the Streptophyte Green Alga Klebsormidium crenulatum
por: Steiner, Philip, et al.
Publicado: (2020)

Winter Nights during Summer Time: Stress Physiological Response to Ice and the Facilitation of Freezing Cytorrhysis by Elastic Cell Wall Components in the Leaves of a Nival Species
por: Stegner, Matthias, et al.
Publicado: (2020)

Rescue of heavy metal effects on cell physiology of the algal model system Micrasterias by divalent ions
por: Volland, Stefanie, et al.
Publicado: (2014)

Solar irradiation levels during simulated long‐ and short‐term heat waves significantly influence heat survival, pigment and ascorbate composition, and free radical scavenging activity in alpine Vaccinium gaultherioides
por: Karadar, Matthias, et al.
Publicado: (2018)

The red alga Tsunamia transpacifica (Stylonematophyceae) from plastic drift shows adaptation to its uncommon habitat in ultrastructure and soluble low molecular weight carbohydrate composition
por: Holzinger, Andreas, et al.
Publicado: (2021)

The Recombinant Inhibitor of DNA Binding Id2 Forms Multimeric Structures via the Helix-Loop-Helix Domain and the Nuclear Export Signal
por: Roschger, Cornelia, et al.
Publicado: (2018)

Gold nanoparticles (AuNPs) impair LPS-driven immune responses by promoting a tolerogenic-like dendritic cell phenotype with altered endosomal structures
por: Michelini, Sara, et al.
Publicado: (2021)

THE PREPARATION OF FROZEN-DRIED TISSUE FOR ELECTRON MICROSCOPY
por: Gersh, Isidore
Publicado: (1956)

Optimization of electron microscopy for human brains with long-term fixation and fixed-frozen sections
por: Liu, Xiao-Bo, et al.
Publicado: (2014)

Winter Frosts Reduce Flower Bud Survival in High-Mountain Plants
por: Wagner, Johanna, et al.
Publicado: (2021)

Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components
por: Boyles, Matthew S. P., et al.
Publicado: (2015)

Nanoparticle-allergen interactions mediate human allergic responses: protein corona characterization and cellular responses
por: Radauer-Preiml, Isabella, et al.
Publicado: (2016)

Application of heat stress in situ demonstrates a protective role of irradiation on photosynthetic performance in alpine plants
por: Buchner, Othmar, et al.
Publicado: (2015)

Heat tolerance of early developmental stages of glacier foreland species in the growth chamber and in the field
por: Marcante, Silvia, et al.
Publicado: (2014)

Heat Acclimation under Drought Stress Induces Antioxidant Enzyme Activity in the Alpine Plant Primula minima
por: Roach, Thomas, et al.
Publicado: (2023)

Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples
por: Lewandowska, Dagmara W., et al.
Publicado: (2017)

Proteomic sample preparation for blast wound characterization
por: Chromy, Brett A, et al.
Publicado: (2014)

Preparation of Cross-Sectional Membrane Samples for Scanning Electron Microscopy Characterizations Using a New Frozen Section Technique
por: Ren, Hongyun, et al.
Publicado: (2023)

New Insights in Potato Leaf Freezing by Infrared Thermography
por: Stegner, Matthias, et al.
Publicado: (2019)

Chloroplast protrusions in leaves of R anunculus glacialis L. respond significantly to different ambient conditions, but are not related to temperature stress
por: MOSER, TIM, et al.
Publicado: (2015)

A random-sampling approach to track cell divisions in time-lapse fluorescence microscopy
por: Amarteifio, Saoirse, et al.
Publicado: (2021)

Miniaturized Sample Preparation for Transmission Electron Microscopy
por: Schmidli, Claudio, et al.
Publicado: (2018)

Mapping genome-wide transcription factor binding sites in frozen tissues
por: Savic, Daniel, et al.
Publicado: (2013)

Formation of chloroplast protrusions and catalase activity in alpine Ranunculus glacialis under elevated temperature and different CO(2)/O(2) ratios
por: Buchner, Othmar, et al.
Publicado: (2015)

High-speed shaking of frozen blood clots for extraction of human and malaria parasite DNA
por: Lundblom, Klara, et al.
Publicado: (2011)

Preparation of blood samples for electron microscopy: The standard protocol
por: Choudhary, Om Prakash, et al.
Publicado: (2021)

Optimized sample preparation for two-dimensional gel electrophoresis of soluble proteins from chicken bursa of Fabricius
por: Wu, Yongping, et al.
Publicado: (2009)

Does winter desiccation account for seasonal increases in supercooling capacity of Norway spruce bud primordia?
por: Kuprian, Edith, et al.
Publicado: (2017)

Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brains
por: Tao-Cheng, Jung-Hwa, et al.
Publicado: (2021)

Electron cryo-microscopy: the frozen frontier of structural biology.
por: Farsad, K., et al.
Publicado: (2001)

ELECTRON MICROSCOPY OF STRUCTURAL DETAIL IN FROZEN BIOLOGICAL SPECIMENS
por: Steere, Russell L.
Publicado: (1957)

Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy?
por: Hänscheid, Thomas, et al.
Publicado: (2009)

Cannot write session to /tmp/vufind_sessions/sess_fmnl8opfjmkpmlh352dkpvcd1h