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A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community

BACKGROUND: Myxobacteria are micropredators in the soil ecosystem with the capacity to move and feed cooperatively. Some myxobacterial strains have been used to control soil-borne fungal phytopathogens. However, interactions among myxobacteria, plant pathogens, and the soil microbiome are largely un...

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Autores principales: Ye, Xianfeng, Li, Zhoukun, Luo, Xue, Wang, Wenhui, Li, Yongkai, Li, Rui, Zhang, Bo, Qiao, Yan, Zhou, Jie, Fan, Jiaqin, Wang, Hui, Huang, Yan, Cao, Hui, Cui, Zhongli, Zhang, Ruifu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137222/
https://www.ncbi.nlm.nih.gov/pubmed/32252828
http://dx.doi.org/10.1186/s40168-020-00824-x
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author Ye, Xianfeng
Li, Zhoukun
Luo, Xue
Wang, Wenhui
Li, Yongkai
Li, Rui
Zhang, Bo
Qiao, Yan
Zhou, Jie
Fan, Jiaqin
Wang, Hui
Huang, Yan
Cao, Hui
Cui, Zhongli
Zhang, Ruifu
author_facet Ye, Xianfeng
Li, Zhoukun
Luo, Xue
Wang, Wenhui
Li, Yongkai
Li, Rui
Zhang, Bo
Qiao, Yan
Zhou, Jie
Fan, Jiaqin
Wang, Hui
Huang, Yan
Cao, Hui
Cui, Zhongli
Zhang, Ruifu
author_sort Ye, Xianfeng
collection PubMed
description BACKGROUND: Myxobacteria are micropredators in the soil ecosystem with the capacity to move and feed cooperatively. Some myxobacterial strains have been used to control soil-borne fungal phytopathogens. However, interactions among myxobacteria, plant pathogens, and the soil microbiome are largely unexplored. In this study, we aimed to investigate the behaviors of the myxobacterium Corallococcus sp. strain EGB in the soil and its effect on the soil microbiome after inoculation for controlling cucumber Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (FOC). RESULTS: A greenhouse and a 2-year field experiment demonstrated that the solid-state fermented strain EGB significantly reduced the cucumber Fusarium wilt by 79.6% (greenhouse), 66.0% (2015, field), and 53.9% (2016, field). Strain EGB adapted to the soil environment well and decreased the abundance of soil-borne FOC efficiently. Spatiotemporal analysis of the soil microbial community showed that strain EGB migrated towards the roots and root exudates of the cucumber plants via chemotaxis. Cooccurrence network analysis of the soil microbiome indicated a decreased modularity and community number but an increased connection number per node after the application of strain EGB. Several predatory bacteria, such as Lysobacter, Microvirga, and Cupriavidus, appearing as hubs or indicators, showed intensive connections with other bacteria. CONCLUSION: The predatory myxobacterium Corallococcus sp. strain EGB controlled cucumber Fusarium wilt by migrating to the plant root and regulating the soil microbial community. This strain has the potential to be developed as a novel biological control agent of soil-borne Fusarium wilt.
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spelling pubmed-71372222020-04-11 A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community Ye, Xianfeng Li, Zhoukun Luo, Xue Wang, Wenhui Li, Yongkai Li, Rui Zhang, Bo Qiao, Yan Zhou, Jie Fan, Jiaqin Wang, Hui Huang, Yan Cao, Hui Cui, Zhongli Zhang, Ruifu Microbiome Research BACKGROUND: Myxobacteria are micropredators in the soil ecosystem with the capacity to move and feed cooperatively. Some myxobacterial strains have been used to control soil-borne fungal phytopathogens. However, interactions among myxobacteria, plant pathogens, and the soil microbiome are largely unexplored. In this study, we aimed to investigate the behaviors of the myxobacterium Corallococcus sp. strain EGB in the soil and its effect on the soil microbiome after inoculation for controlling cucumber Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (FOC). RESULTS: A greenhouse and a 2-year field experiment demonstrated that the solid-state fermented strain EGB significantly reduced the cucumber Fusarium wilt by 79.6% (greenhouse), 66.0% (2015, field), and 53.9% (2016, field). Strain EGB adapted to the soil environment well and decreased the abundance of soil-borne FOC efficiently. Spatiotemporal analysis of the soil microbial community showed that strain EGB migrated towards the roots and root exudates of the cucumber plants via chemotaxis. Cooccurrence network analysis of the soil microbiome indicated a decreased modularity and community number but an increased connection number per node after the application of strain EGB. Several predatory bacteria, such as Lysobacter, Microvirga, and Cupriavidus, appearing as hubs or indicators, showed intensive connections with other bacteria. CONCLUSION: The predatory myxobacterium Corallococcus sp. strain EGB controlled cucumber Fusarium wilt by migrating to the plant root and regulating the soil microbial community. This strain has the potential to be developed as a novel biological control agent of soil-borne Fusarium wilt. BioMed Central 2020-04-06 /pmc/articles/PMC7137222/ /pubmed/32252828 http://dx.doi.org/10.1186/s40168-020-00824-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Ye, Xianfeng
Li, Zhoukun
Luo, Xue
Wang, Wenhui
Li, Yongkai
Li, Rui
Zhang, Bo
Qiao, Yan
Zhou, Jie
Fan, Jiaqin
Wang, Hui
Huang, Yan
Cao, Hui
Cui, Zhongli
Zhang, Ruifu
A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community
title A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community
title_full A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community
title_fullStr A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community
title_full_unstemmed A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community
title_short A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community
title_sort predatory myxobacterium controls cucumber fusarium wilt by regulating the soil microbial community
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137222/
https://www.ncbi.nlm.nih.gov/pubmed/32252828
http://dx.doi.org/10.1186/s40168-020-00824-x
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