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Genomic investigation of emerging zoonotic pathogen Shewanella xiamenensis
OBJECTIVE: Shewanella xiamenensis is an emerging zoonotic pathogen commonly found in aquatic ecosystem. Clustered regularly interspaced short palindromic repeats (CRISPR) and (CRISPR)-associated gene systems act as adaptive immune system of prokaryotes. Recently, growing evidence suggested their rol...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137373/ https://www.ncbi.nlm.nih.gov/pubmed/32269948 http://dx.doi.org/10.4103/tcmj.tcmj_69_19 |
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author | Wang, Jui-Hsing Tseng, Shu-Ying Tung, Kwong-Chung |
author_facet | Wang, Jui-Hsing Tseng, Shu-Ying Tung, Kwong-Chung |
author_sort | Wang, Jui-Hsing |
collection | PubMed |
description | OBJECTIVE: Shewanella xiamenensis is an emerging zoonotic pathogen commonly found in aquatic ecosystem. Clustered regularly interspaced short palindromic repeats (CRISPR) and (CRISPR)-associated gene systems act as adaptive immune system of prokaryotes. Recently, growing evidence suggested their role in bacterial virulence and resistance. Despite its medical importance, little is known about the genomic characteristics of S. xiamenensis. MATERIALS AND METHODS: Strain ZYW6 was isolated from Epinephelus awoara. We sequenced the 16S rRNA gene and blast against the GenBank bacterial database. Antibiotic susceptibility tests and interpretation were performed by automatic VITEK 2 system. We extracted the genomic DNA with QIAGEN Genomic-tip 100/G kit and QIAGEN Genomic DNA Buffer Set. Whole-genome shotgun sequencing was performed using the Illumina MiSeq sequencer. To identify the CRISPR-Cas System in the genome of S. xiamenensis ZYW6, the Integrated Microbial Genomes and Microbiomes and CRISPRFinder were used. RESULTS: We characterized the genome of a S. xiamenensis strain. The genome is 4,765,190 bp in length and encodes 4262 open-reading frames. Type I CRISPR-Cas system and serine biosynthesis genes were identified. CONCLUSION: Our results demonstrate the genetic structure of CRISPR-Cas system, l-serine synthesis, and oxacillinase in S. xiamenensis. The report of antibiotics resistance genes in the study might indicate a possible reservoir of antimicrobial drug resistance determinants in food animal, resulting in potential infection source. The findings provide insights into the structure and composition of CRISPR-Cas system in S. xiamenensis and foundation for future biological validation. |
format | Online Article Text |
id | pubmed-7137373 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-71373732020-04-08 Genomic investigation of emerging zoonotic pathogen Shewanella xiamenensis Wang, Jui-Hsing Tseng, Shu-Ying Tung, Kwong-Chung Tzu Chi Med J Original Article OBJECTIVE: Shewanella xiamenensis is an emerging zoonotic pathogen commonly found in aquatic ecosystem. Clustered regularly interspaced short palindromic repeats (CRISPR) and (CRISPR)-associated gene systems act as adaptive immune system of prokaryotes. Recently, growing evidence suggested their role in bacterial virulence and resistance. Despite its medical importance, little is known about the genomic characteristics of S. xiamenensis. MATERIALS AND METHODS: Strain ZYW6 was isolated from Epinephelus awoara. We sequenced the 16S rRNA gene and blast against the GenBank bacterial database. Antibiotic susceptibility tests and interpretation were performed by automatic VITEK 2 system. We extracted the genomic DNA with QIAGEN Genomic-tip 100/G kit and QIAGEN Genomic DNA Buffer Set. Whole-genome shotgun sequencing was performed using the Illumina MiSeq sequencer. To identify the CRISPR-Cas System in the genome of S. xiamenensis ZYW6, the Integrated Microbial Genomes and Microbiomes and CRISPRFinder were used. RESULTS: We characterized the genome of a S. xiamenensis strain. The genome is 4,765,190 bp in length and encodes 4262 open-reading frames. Type I CRISPR-Cas system and serine biosynthesis genes were identified. CONCLUSION: Our results demonstrate the genetic structure of CRISPR-Cas system, l-serine synthesis, and oxacillinase in S. xiamenensis. The report of antibiotics resistance genes in the study might indicate a possible reservoir of antimicrobial drug resistance determinants in food animal, resulting in potential infection source. The findings provide insights into the structure and composition of CRISPR-Cas system in S. xiamenensis and foundation for future biological validation. Wolters Kluwer - Medknow 2019-06-13 /pmc/articles/PMC7137373/ /pubmed/32269948 http://dx.doi.org/10.4103/tcmj.tcmj_69_19 Text en Copyright: © 2019 Tzu Chi Medical Journal http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Wang, Jui-Hsing Tseng, Shu-Ying Tung, Kwong-Chung Genomic investigation of emerging zoonotic pathogen Shewanella xiamenensis |
title | Genomic investigation of emerging zoonotic pathogen Shewanella xiamenensis |
title_full | Genomic investigation of emerging zoonotic pathogen Shewanella xiamenensis |
title_fullStr | Genomic investigation of emerging zoonotic pathogen Shewanella xiamenensis |
title_full_unstemmed | Genomic investigation of emerging zoonotic pathogen Shewanella xiamenensis |
title_short | Genomic investigation of emerging zoonotic pathogen Shewanella xiamenensis |
title_sort | genomic investigation of emerging zoonotic pathogen shewanella xiamenensis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137373/ https://www.ncbi.nlm.nih.gov/pubmed/32269948 http://dx.doi.org/10.4103/tcmj.tcmj_69_19 |
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