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Tobacco etch virus (TEV) protease with multiple mutations to improve solubility and reduce self‐cleavage exhibits enhanced enzymatic activity

Tobacco etch virus (TEV) protease is a 27‐kDa catalytic domain of the polyprotein nuclear inclusion a (NIa) in TEV, which recognizes the specific amino acid sequence ENLYFQG/S and cleaves between Q and G/S. Despite its substrate specificity, its use is limited by its autoinactivation through self‐cl...

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Detalles Bibliográficos
Autores principales: Nam, Heejin, Hwang, Beom J., Choi, Deog‐Young, Shin, Sooim, Choi, Moonsung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137792/
https://www.ncbi.nlm.nih.gov/pubmed/32129006
http://dx.doi.org/10.1002/2211-5463.12828

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