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Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease
Meibum lipids form a lipid layer on the outermost side of the tear film and function to prevent water evaporation and reduce surface tension. (O-Acyl)-ω-hydroxy fatty acids (OAHFAs), a subclass of these lipids, are thought to be involved in connecting the lipid and aqueous layers in tears, although...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138607/ https://www.ncbi.nlm.nih.gov/pubmed/32252890 http://dx.doi.org/10.7554/eLife.53582 |
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author | Miyamoto, Masatoshi Sassa, Takayuki Sawai, Megumi Kihara, Akio |
author_facet | Miyamoto, Masatoshi Sassa, Takayuki Sawai, Megumi Kihara, Akio |
author_sort | Miyamoto, Masatoshi |
collection | PubMed |
description | Meibum lipids form a lipid layer on the outermost side of the tear film and function to prevent water evaporation and reduce surface tension. (O-Acyl)-ω-hydroxy fatty acids (OAHFAs), a subclass of these lipids, are thought to be involved in connecting the lipid and aqueous layers in tears, although their actual function and synthesis pathway have to date remained unclear. Here, we reveal that the fatty acid ω-hydroxylase Cyp4f39 is involved in OAHFA production. Cyp4f39-deficient mice exhibited damaged corneal epithelium and shortening of tear film break-up time, both indicative of dry eye disease. In addition, tears accumulated on the lower eyelid side, indicating increased tear surface tension. In Cyp4f39-deficient mice, the production of wax diesters (type 1ω and 2ω) and cholesteryl OAHFAs was also impaired. These OAHFA derivatives show intermediate polarity among meibum lipids, suggesting that OAHFAs and their derivatives contribute to lipid polarity gradient formation for tear film stabilization. |
format | Online Article Text |
id | pubmed-7138607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-71386072020-04-08 Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease Miyamoto, Masatoshi Sassa, Takayuki Sawai, Megumi Kihara, Akio eLife Biochemistry and Chemical Biology Meibum lipids form a lipid layer on the outermost side of the tear film and function to prevent water evaporation and reduce surface tension. (O-Acyl)-ω-hydroxy fatty acids (OAHFAs), a subclass of these lipids, are thought to be involved in connecting the lipid and aqueous layers in tears, although their actual function and synthesis pathway have to date remained unclear. Here, we reveal that the fatty acid ω-hydroxylase Cyp4f39 is involved in OAHFA production. Cyp4f39-deficient mice exhibited damaged corneal epithelium and shortening of tear film break-up time, both indicative of dry eye disease. In addition, tears accumulated on the lower eyelid side, indicating increased tear surface tension. In Cyp4f39-deficient mice, the production of wax diesters (type 1ω and 2ω) and cholesteryl OAHFAs was also impaired. These OAHFA derivatives show intermediate polarity among meibum lipids, suggesting that OAHFAs and their derivatives contribute to lipid polarity gradient formation for tear film stabilization. eLife Sciences Publications, Ltd 2020-04-07 /pmc/articles/PMC7138607/ /pubmed/32252890 http://dx.doi.org/10.7554/eLife.53582 Text en © 2020, Miyamoto et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry and Chemical Biology Miyamoto, Masatoshi Sassa, Takayuki Sawai, Megumi Kihara, Akio Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease |
title | Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease |
title_full | Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease |
title_fullStr | Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease |
title_full_unstemmed | Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease |
title_short | Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease |
title_sort | lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138607/ https://www.ncbi.nlm.nih.gov/pubmed/32252890 http://dx.doi.org/10.7554/eLife.53582 |
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