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Complex intron generation in the yeast genus Lipomyces

In primary transcripts of eukaryotic nuclear genes, coding sequences are often interrupted by U2-type introns. Such intervening sequences can constitute complex introns excised by consecutive splicing reactions. The origin of spliceosomal introns is a vexing problem. Sequence variation existent acro...

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Autores principales: Ág, Norbert, Kavalecz, Napsugár, Pénzes, Fruzsina, Karaffa, Levente, Scazzocchio, Claudio, Flipphi, Michel, Fekete, Erzsébet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138796/
https://www.ncbi.nlm.nih.gov/pubmed/32265493
http://dx.doi.org/10.1038/s41598-020-63239-6
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author Ág, Norbert
Kavalecz, Napsugár
Pénzes, Fruzsina
Karaffa, Levente
Scazzocchio, Claudio
Flipphi, Michel
Fekete, Erzsébet
author_facet Ág, Norbert
Kavalecz, Napsugár
Pénzes, Fruzsina
Karaffa, Levente
Scazzocchio, Claudio
Flipphi, Michel
Fekete, Erzsébet
author_sort Ág, Norbert
collection PubMed
description In primary transcripts of eukaryotic nuclear genes, coding sequences are often interrupted by U2-type introns. Such intervening sequences can constitute complex introns excised by consecutive splicing reactions. The origin of spliceosomal introns is a vexing problem. Sequence variation existent across fungal taxa provides means to study their structure and evolution. In one class of complex introns called [D] stwintrons, an (internal) U2 intron is nested within the 5'-donor element of another (external) U2 intron. In the gene for a reticulon-like protein in species of the ascomycete yeast genus Lipomyces, the most 5' terminal intron position is occupied by one of three complex intervening sequences consistent of differently nested U2 intron units, as demonstrated in L. lipofer, L. suomiensis, and L. starkeyi. In L. starkeyi, the donor elements of the constituent introns are abutting and the complex intervening sequence can be excised alternatively either with one standard splicing reaction or, as a [D] stwintron, by two consecutive reactions. Our work suggests how [D] stwintrons could emerge by the appearance of new functional splice sites within an extant intron. The stepwise stwintronisation mechanism may involve duplication of the functional intron donor element of the ancestor intron.
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spelling pubmed-71387962020-04-11 Complex intron generation in the yeast genus Lipomyces Ág, Norbert Kavalecz, Napsugár Pénzes, Fruzsina Karaffa, Levente Scazzocchio, Claudio Flipphi, Michel Fekete, Erzsébet Sci Rep Article In primary transcripts of eukaryotic nuclear genes, coding sequences are often interrupted by U2-type introns. Such intervening sequences can constitute complex introns excised by consecutive splicing reactions. The origin of spliceosomal introns is a vexing problem. Sequence variation existent across fungal taxa provides means to study their structure and evolution. In one class of complex introns called [D] stwintrons, an (internal) U2 intron is nested within the 5'-donor element of another (external) U2 intron. In the gene for a reticulon-like protein in species of the ascomycete yeast genus Lipomyces, the most 5' terminal intron position is occupied by one of three complex intervening sequences consistent of differently nested U2 intron units, as demonstrated in L. lipofer, L. suomiensis, and L. starkeyi. In L. starkeyi, the donor elements of the constituent introns are abutting and the complex intervening sequence can be excised alternatively either with one standard splicing reaction or, as a [D] stwintron, by two consecutive reactions. Our work suggests how [D] stwintrons could emerge by the appearance of new functional splice sites within an extant intron. The stepwise stwintronisation mechanism may involve duplication of the functional intron donor element of the ancestor intron. Nature Publishing Group UK 2020-04-07 /pmc/articles/PMC7138796/ /pubmed/32265493 http://dx.doi.org/10.1038/s41598-020-63239-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ág, Norbert
Kavalecz, Napsugár
Pénzes, Fruzsina
Karaffa, Levente
Scazzocchio, Claudio
Flipphi, Michel
Fekete, Erzsébet
Complex intron generation in the yeast genus Lipomyces
title Complex intron generation in the yeast genus Lipomyces
title_full Complex intron generation in the yeast genus Lipomyces
title_fullStr Complex intron generation in the yeast genus Lipomyces
title_full_unstemmed Complex intron generation in the yeast genus Lipomyces
title_short Complex intron generation in the yeast genus Lipomyces
title_sort complex intron generation in the yeast genus lipomyces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138796/
https://www.ncbi.nlm.nih.gov/pubmed/32265493
http://dx.doi.org/10.1038/s41598-020-63239-6
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