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miR-802 participates in the inflammatory process of inflammatory bowel disease by suppressing SOCS5

The present study aims to reveal the detailed molecular mechanism of microRNA (miR)-802 in the progression of inflammatory bowel disease (IBD). IBD tissues were obtained from IBD patients, followed by CD4(+) cells isolation. Then, qRT-PCR and ELISA were used to detect the expression of miR-802, supp...

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Detalles Bibliográficos
Autores principales: Yao, Jun, Gao, Ruoyu, Luo, Minghan, Li, Defeng, Guo, Liliangzi, Yu, Zichao, Xiong, Feng, Wei, Cheng, Wu, Benhua, Xu, Zhenglei, Zhang, Dingguo, Wang, Jianyao, Wang, Lisheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138906/
https://www.ncbi.nlm.nih.gov/pubmed/32211804
http://dx.doi.org/10.1042/BSR20192257
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author Yao, Jun
Gao, Ruoyu
Luo, Minghan
Li, Defeng
Guo, Liliangzi
Yu, Zichao
Xiong, Feng
Wei, Cheng
Wu, Benhua
Xu, Zhenglei
Zhang, Dingguo
Wang, Jianyao
Wang, Lisheng
author_facet Yao, Jun
Gao, Ruoyu
Luo, Minghan
Li, Defeng
Guo, Liliangzi
Yu, Zichao
Xiong, Feng
Wei, Cheng
Wu, Benhua
Xu, Zhenglei
Zhang, Dingguo
Wang, Jianyao
Wang, Lisheng
author_sort Yao, Jun
collection PubMed
description The present study aims to reveal the detailed molecular mechanism of microRNA (miR)-802 in the progression of inflammatory bowel disease (IBD). IBD tissues were obtained from IBD patients, followed by CD4(+) cells isolation. Then, qRT-PCR and ELISA were used to detect the expression of miR-802, suppressor of cytokine signaling 5 (SOCS5), interleukin (IL)-17A and tumor necrosis factor (TNF)-α. Transfection of miR-802 mimics and miR-802 inhibitor in CD4(+) cells was detected by Western blot. TargetScan and luciferase reporter assay were used to detect the relationship between SOCS5 and miR-802. Finally, colitis mice model was established to verify whether miR-802 inhibitor was involved in the protective effect of colonic mucosa. The miR-802 was highly expressed in inflamed mucosa and PBMC cells of IBD. The highest expression of miR-802 was observed in CD4(+) T cells based on different immune cell subsets analysis. SOCS5 was the target gene of miR-802. The mice model experiments showed that blockade of miR-802 could alleviate mice colitis. Our study suggests that up-regulation of miR-802 plays an important role in inflammatory process of IBD via targeting SOCS5. Moreover, the differentiation of Th17 and secretion of TNF-α in IBD could be stimulated by miR-802.
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spelling pubmed-71389062020-04-10 miR-802 participates in the inflammatory process of inflammatory bowel disease by suppressing SOCS5 Yao, Jun Gao, Ruoyu Luo, Minghan Li, Defeng Guo, Liliangzi Yu, Zichao Xiong, Feng Wei, Cheng Wu, Benhua Xu, Zhenglei Zhang, Dingguo Wang, Jianyao Wang, Lisheng Biosci Rep Biochemical Techniques & Resources The present study aims to reveal the detailed molecular mechanism of microRNA (miR)-802 in the progression of inflammatory bowel disease (IBD). IBD tissues were obtained from IBD patients, followed by CD4(+) cells isolation. Then, qRT-PCR and ELISA were used to detect the expression of miR-802, suppressor of cytokine signaling 5 (SOCS5), interleukin (IL)-17A and tumor necrosis factor (TNF)-α. Transfection of miR-802 mimics and miR-802 inhibitor in CD4(+) cells was detected by Western blot. TargetScan and luciferase reporter assay were used to detect the relationship between SOCS5 and miR-802. Finally, colitis mice model was established to verify whether miR-802 inhibitor was involved in the protective effect of colonic mucosa. The miR-802 was highly expressed in inflamed mucosa and PBMC cells of IBD. The highest expression of miR-802 was observed in CD4(+) T cells based on different immune cell subsets analysis. SOCS5 was the target gene of miR-802. The mice model experiments showed that blockade of miR-802 could alleviate mice colitis. Our study suggests that up-regulation of miR-802 plays an important role in inflammatory process of IBD via targeting SOCS5. Moreover, the differentiation of Th17 and secretion of TNF-α in IBD could be stimulated by miR-802. Portland Press Ltd. 2020-04-07 /pmc/articles/PMC7138906/ /pubmed/32211804 http://dx.doi.org/10.1042/BSR20192257 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
spellingShingle Biochemical Techniques & Resources
Yao, Jun
Gao, Ruoyu
Luo, Minghan
Li, Defeng
Guo, Liliangzi
Yu, Zichao
Xiong, Feng
Wei, Cheng
Wu, Benhua
Xu, Zhenglei
Zhang, Dingguo
Wang, Jianyao
Wang, Lisheng
miR-802 participates in the inflammatory process of inflammatory bowel disease by suppressing SOCS5
title miR-802 participates in the inflammatory process of inflammatory bowel disease by suppressing SOCS5
title_full miR-802 participates in the inflammatory process of inflammatory bowel disease by suppressing SOCS5
title_fullStr miR-802 participates in the inflammatory process of inflammatory bowel disease by suppressing SOCS5
title_full_unstemmed miR-802 participates in the inflammatory process of inflammatory bowel disease by suppressing SOCS5
title_short miR-802 participates in the inflammatory process of inflammatory bowel disease by suppressing SOCS5
title_sort mir-802 participates in the inflammatory process of inflammatory bowel disease by suppressing socs5
topic Biochemical Techniques & Resources
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138906/
https://www.ncbi.nlm.nih.gov/pubmed/32211804
http://dx.doi.org/10.1042/BSR20192257
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