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Hollow fiber vitrification allows cryopreservation of embryos with compromised cryotolerance

PURPOSE: This study aims to demonstrate vitrification methods that provide reliable cryopreservation for embryos with compromised cryotolerance. METHODS: Two‐cell stage mouse embryos and in vitro produced porcine embryos were vitrified using the hollow fiber vitrification (HFV) and Cryotop (CT) meth...

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Autores principales: Uchikura, Ayuko, Matsunari, Hitomi, Maehara, Miki, Yonamine, Shiori, Wakayama, Sayaka, Wakayama, Teruhiko, Nagashima, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138943/
https://www.ncbi.nlm.nih.gov/pubmed/32273819
http://dx.doi.org/10.1002/rmb2.12312
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author Uchikura, Ayuko
Matsunari, Hitomi
Maehara, Miki
Yonamine, Shiori
Wakayama, Sayaka
Wakayama, Teruhiko
Nagashima, Hiroshi
author_facet Uchikura, Ayuko
Matsunari, Hitomi
Maehara, Miki
Yonamine, Shiori
Wakayama, Sayaka
Wakayama, Teruhiko
Nagashima, Hiroshi
author_sort Uchikura, Ayuko
collection PubMed
description PURPOSE: This study aims to demonstrate vitrification methods that provide reliable cryopreservation for embryos with compromised cryotolerance. METHODS: Two‐cell stage mouse embryos and in vitro produced porcine embryos were vitrified using the hollow fiber vitrification (HFV) and Cryotop (CT) methods. The performance of these two methods was compared by the viability of the vitrified‐rewarmed embryos. RESULTS: Regardless of the method used, 100% of the mouse 2‐cell embryos developed successfully after vitrification‐rewarming into the blastocyst stage, whereas vitrification tests using porcine morulae with the HFV method produced significantly better results. The developmental rates of vitrified porcine morula into the blastocyst stage, as well as blastocyst cell number, were 90.3% and 112.3 ± 6.9 in the HFV group compared with 63.4% and 89.5 ± 8.1 in the CT group (P < .05). Vitrification tests using 4‐ to 8‐cell porcine embryos resulted in development into the blastocyst stage (45.5%) in the HFV group alone, demonstrating its better efficacy. The HFV method did not impair embryo viability, even after spontaneous rewarming at room temperature for vitrified embryos, which is generally considered a contraindication. CONCLUSION: Vitrification test using embryos with compromised cryotolerance allows for more precise determining of effective cryopreservation methods and devices.
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spelling pubmed-71389432020-04-09 Hollow fiber vitrification allows cryopreservation of embryos with compromised cryotolerance Uchikura, Ayuko Matsunari, Hitomi Maehara, Miki Yonamine, Shiori Wakayama, Sayaka Wakayama, Teruhiko Nagashima, Hiroshi Reprod Med Biol Original Articles PURPOSE: This study aims to demonstrate vitrification methods that provide reliable cryopreservation for embryos with compromised cryotolerance. METHODS: Two‐cell stage mouse embryos and in vitro produced porcine embryos were vitrified using the hollow fiber vitrification (HFV) and Cryotop (CT) methods. The performance of these two methods was compared by the viability of the vitrified‐rewarmed embryos. RESULTS: Regardless of the method used, 100% of the mouse 2‐cell embryos developed successfully after vitrification‐rewarming into the blastocyst stage, whereas vitrification tests using porcine morulae with the HFV method produced significantly better results. The developmental rates of vitrified porcine morula into the blastocyst stage, as well as blastocyst cell number, were 90.3% and 112.3 ± 6.9 in the HFV group compared with 63.4% and 89.5 ± 8.1 in the CT group (P < .05). Vitrification tests using 4‐ to 8‐cell porcine embryos resulted in development into the blastocyst stage (45.5%) in the HFV group alone, demonstrating its better efficacy. The HFV method did not impair embryo viability, even after spontaneous rewarming at room temperature for vitrified embryos, which is generally considered a contraindication. CONCLUSION: Vitrification test using embryos with compromised cryotolerance allows for more precise determining of effective cryopreservation methods and devices. John Wiley and Sons Inc. 2019-12-21 /pmc/articles/PMC7138943/ /pubmed/32273819 http://dx.doi.org/10.1002/rmb2.12312 Text en © 2019 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Uchikura, Ayuko
Matsunari, Hitomi
Maehara, Miki
Yonamine, Shiori
Wakayama, Sayaka
Wakayama, Teruhiko
Nagashima, Hiroshi
Hollow fiber vitrification allows cryopreservation of embryos with compromised cryotolerance
title Hollow fiber vitrification allows cryopreservation of embryos with compromised cryotolerance
title_full Hollow fiber vitrification allows cryopreservation of embryos with compromised cryotolerance
title_fullStr Hollow fiber vitrification allows cryopreservation of embryos with compromised cryotolerance
title_full_unstemmed Hollow fiber vitrification allows cryopreservation of embryos with compromised cryotolerance
title_short Hollow fiber vitrification allows cryopreservation of embryos with compromised cryotolerance
title_sort hollow fiber vitrification allows cryopreservation of embryos with compromised cryotolerance
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138943/
https://www.ncbi.nlm.nih.gov/pubmed/32273819
http://dx.doi.org/10.1002/rmb2.12312
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