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Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India
Molecular surveillance of the drug resistance genes in parasite can be used for monitoring/surveillance of drug resistance in endemic malaria areas. Here we report the prevalence of single nucleotide polymorphisms (SNPs) in dihydrofolate reductase (dhfr) gene in nucleotide sequence of Plasmodium fal...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139110/ https://www.ncbi.nlm.nih.gov/pubmed/32280801 http://dx.doi.org/10.1016/j.heliyon.2020.e03715 |
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author | Kumar, Amit Gahlawat, S.K. Singh, Vineeta |
author_facet | Kumar, Amit Gahlawat, S.K. Singh, Vineeta |
author_sort | Kumar, Amit |
collection | PubMed |
description | Molecular surveillance of the drug resistance genes in parasite can be used for monitoring/surveillance of drug resistance in endemic malaria areas. Here we report the prevalence of single nucleotide polymorphisms (SNPs) in dihydrofolate reductase (dhfr) gene in nucleotide sequence of Plasmodium falciparum from different regions in India. We found markedly prevalent mutants evident in P. falciparum infections N51I, C59R, 108N and I164L. Our results indicate that P. falciparum populations in the regions show an increase in the prevalence of polymorphisms, most likely reflecting different selective pressures found in humans and mosquitoes. Molecular surveillance can serve as a useful tool to monitor the prevalence/emergence of resistant genotypes within endemic populations and can serve for determining the efficacy of antimalarial drugs. |
format | Online Article Text |
id | pubmed-7139110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-71391102020-04-10 Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India Kumar, Amit Gahlawat, S.K. Singh, Vineeta Heliyon Article Molecular surveillance of the drug resistance genes in parasite can be used for monitoring/surveillance of drug resistance in endemic malaria areas. Here we report the prevalence of single nucleotide polymorphisms (SNPs) in dihydrofolate reductase (dhfr) gene in nucleotide sequence of Plasmodium falciparum from different regions in India. We found markedly prevalent mutants evident in P. falciparum infections N51I, C59R, 108N and I164L. Our results indicate that P. falciparum populations in the regions show an increase in the prevalence of polymorphisms, most likely reflecting different selective pressures found in humans and mosquitoes. Molecular surveillance can serve as a useful tool to monitor the prevalence/emergence of resistant genotypes within endemic populations and can serve for determining the efficacy of antimalarial drugs. Elsevier 2020-04-06 /pmc/articles/PMC7139110/ /pubmed/32280801 http://dx.doi.org/10.1016/j.heliyon.2020.e03715 Text en © 2020 Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Kumar, Amit Gahlawat, S.K. Singh, Vineeta Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India |
title | Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India |
title_full | Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India |
title_fullStr | Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India |
title_full_unstemmed | Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India |
title_short | Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India |
title_sort | comparative analysis of plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of india |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139110/ https://www.ncbi.nlm.nih.gov/pubmed/32280801 http://dx.doi.org/10.1016/j.heliyon.2020.e03715 |
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