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Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India

Molecular surveillance of the drug resistance genes in parasite can be used for monitoring/surveillance of drug resistance in endemic malaria areas. Here we report the prevalence of single nucleotide polymorphisms (SNPs) in dihydrofolate reductase (dhfr) gene in nucleotide sequence of Plasmodium fal...

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Detalles Bibliográficos
Autores principales: Kumar, Amit, Gahlawat, S.K., Singh, Vineeta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139110/
https://www.ncbi.nlm.nih.gov/pubmed/32280801
http://dx.doi.org/10.1016/j.heliyon.2020.e03715
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author Kumar, Amit
Gahlawat, S.K.
Singh, Vineeta
author_facet Kumar, Amit
Gahlawat, S.K.
Singh, Vineeta
author_sort Kumar, Amit
collection PubMed
description Molecular surveillance of the drug resistance genes in parasite can be used for monitoring/surveillance of drug resistance in endemic malaria areas. Here we report the prevalence of single nucleotide polymorphisms (SNPs) in dihydrofolate reductase (dhfr) gene in nucleotide sequence of Plasmodium falciparum from different regions in India. We found markedly prevalent mutants evident in P. falciparum infections N51I, C59R, 108N and I164L. Our results indicate that P. falciparum populations in the regions show an increase in the prevalence of polymorphisms, most likely reflecting different selective pressures found in humans and mosquitoes. Molecular surveillance can serve as a useful tool to monitor the prevalence/emergence of resistant genotypes within endemic populations and can serve for determining the efficacy of antimalarial drugs.
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spelling pubmed-71391102020-04-10 Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India Kumar, Amit Gahlawat, S.K. Singh, Vineeta Heliyon Article Molecular surveillance of the drug resistance genes in parasite can be used for monitoring/surveillance of drug resistance in endemic malaria areas. Here we report the prevalence of single nucleotide polymorphisms (SNPs) in dihydrofolate reductase (dhfr) gene in nucleotide sequence of Plasmodium falciparum from different regions in India. We found markedly prevalent mutants evident in P. falciparum infections N51I, C59R, 108N and I164L. Our results indicate that P. falciparum populations in the regions show an increase in the prevalence of polymorphisms, most likely reflecting different selective pressures found in humans and mosquitoes. Molecular surveillance can serve as a useful tool to monitor the prevalence/emergence of resistant genotypes within endemic populations and can serve for determining the efficacy of antimalarial drugs. Elsevier 2020-04-06 /pmc/articles/PMC7139110/ /pubmed/32280801 http://dx.doi.org/10.1016/j.heliyon.2020.e03715 Text en © 2020 Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Kumar, Amit
Gahlawat, S.K.
Singh, Vineeta
Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India
title Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India
title_full Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India
title_fullStr Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India
title_full_unstemmed Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India
title_short Comparative analysis of Plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of India
title_sort comparative analysis of plasmodium falciparum dihydrofolate-reductase gene sequences from different regions of india
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139110/
https://www.ncbi.nlm.nih.gov/pubmed/32280801
http://dx.doi.org/10.1016/j.heliyon.2020.e03715
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