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Bio responsive self-assembly of Au-miRNAs for targeted cancer theranostics

BACKGROUND: MicroRNA (miRNA) therapeutics are a promising approach to cancer treatment. However, this method faces considerable challenges to achieve tissue-specific, efficient, and safe delivery of miRNAs in vivo. METHODS: Herein, we developed a miRNA delivery system based on the in situ self-assem...

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Autores principales: Cai, Weijuan, Feng, Huan, Yin, Liang, Wang, Maonan, Jiang, Xuerui, Qin, Zhaojian, Liu, Weiwei, Li, Chunmei, Jiang, Hui, Weizmann, Yossi, Wang, Xuemei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139156/
https://www.ncbi.nlm.nih.gov/pubmed/32276223
http://dx.doi.org/10.1016/j.ebiom.2020.102740
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author Cai, Weijuan
Feng, Huan
Yin, Liang
Wang, Maonan
Jiang, Xuerui
Qin, Zhaojian
Liu, Weiwei
Li, Chunmei
Jiang, Hui
Weizmann, Yossi
Wang, Xuemei
author_facet Cai, Weijuan
Feng, Huan
Yin, Liang
Wang, Maonan
Jiang, Xuerui
Qin, Zhaojian
Liu, Weiwei
Li, Chunmei
Jiang, Hui
Weizmann, Yossi
Wang, Xuemei
author_sort Cai, Weijuan
collection PubMed
description BACKGROUND: MicroRNA (miRNA) therapeutics are a promising approach to cancer treatment. However, this method faces considerable challenges to achieve tissue-specific, efficient, and safe delivery of miRNAs in vivo. METHODS: Herein, we developed a miRNA delivery system based on the in situ self-assembly of Au-miRNA nanocomplexes (Au-miRNA NCs). Within the cancer microenvironment, we constructed in situ self-assembled Au-miRNA NCs by coincubating gold salt and tumor suppressor mimics, such as let-7a, miRNA-34a, and miRNA-200a. FINDINGS: The in vitro experiments demonstrated that characteristic in situ self-assembled Au-miRNA NCs were present in cancer cells and can be taken up to inhibit the proliferation of cancer cells effectively. Most importantly, as proven in subcutaneous tumor treatment models, Au-miRNA NCs were especially useful for accurate target imaging and tumor suppression, with significantly enhanced antitumor effects for combination therapy. INTERPRETATION: These observations highlight that a new strategy for the in situ biosynthesis of Au-let-7a NCs, Au-miR-34a NCs, and Au-miR-200a NCs is feasible, and this may assist in the delivery of more miRNA to tumor cells for cancer treatment. This work opens up new opportunities for the development of miRNA tumor therapy strategies. FUNDING: National Natural Science Foundation of China (91753106); Primary Research & Development Plan of Jiangsu Province (BE2019716); National Key Research and Development Program of China (2017YFA0205300).
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spelling pubmed-71391562020-04-10 Bio responsive self-assembly of Au-miRNAs for targeted cancer theranostics Cai, Weijuan Feng, Huan Yin, Liang Wang, Maonan Jiang, Xuerui Qin, Zhaojian Liu, Weiwei Li, Chunmei Jiang, Hui Weizmann, Yossi Wang, Xuemei EBioMedicine Research paper BACKGROUND: MicroRNA (miRNA) therapeutics are a promising approach to cancer treatment. However, this method faces considerable challenges to achieve tissue-specific, efficient, and safe delivery of miRNAs in vivo. METHODS: Herein, we developed a miRNA delivery system based on the in situ self-assembly of Au-miRNA nanocomplexes (Au-miRNA NCs). Within the cancer microenvironment, we constructed in situ self-assembled Au-miRNA NCs by coincubating gold salt and tumor suppressor mimics, such as let-7a, miRNA-34a, and miRNA-200a. FINDINGS: The in vitro experiments demonstrated that characteristic in situ self-assembled Au-miRNA NCs were present in cancer cells and can be taken up to inhibit the proliferation of cancer cells effectively. Most importantly, as proven in subcutaneous tumor treatment models, Au-miRNA NCs were especially useful for accurate target imaging and tumor suppression, with significantly enhanced antitumor effects for combination therapy. INTERPRETATION: These observations highlight that a new strategy for the in situ biosynthesis of Au-let-7a NCs, Au-miR-34a NCs, and Au-miR-200a NCs is feasible, and this may assist in the delivery of more miRNA to tumor cells for cancer treatment. This work opens up new opportunities for the development of miRNA tumor therapy strategies. FUNDING: National Natural Science Foundation of China (91753106); Primary Research & Development Plan of Jiangsu Province (BE2019716); National Key Research and Development Program of China (2017YFA0205300). Elsevier 2020-04-07 /pmc/articles/PMC7139156/ /pubmed/32276223 http://dx.doi.org/10.1016/j.ebiom.2020.102740 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research paper
Cai, Weijuan
Feng, Huan
Yin, Liang
Wang, Maonan
Jiang, Xuerui
Qin, Zhaojian
Liu, Weiwei
Li, Chunmei
Jiang, Hui
Weizmann, Yossi
Wang, Xuemei
Bio responsive self-assembly of Au-miRNAs for targeted cancer theranostics
title Bio responsive self-assembly of Au-miRNAs for targeted cancer theranostics
title_full Bio responsive self-assembly of Au-miRNAs for targeted cancer theranostics
title_fullStr Bio responsive self-assembly of Au-miRNAs for targeted cancer theranostics
title_full_unstemmed Bio responsive self-assembly of Au-miRNAs for targeted cancer theranostics
title_short Bio responsive self-assembly of Au-miRNAs for targeted cancer theranostics
title_sort bio responsive self-assembly of au-mirnas for targeted cancer theranostics
topic Research paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139156/
https://www.ncbi.nlm.nih.gov/pubmed/32276223
http://dx.doi.org/10.1016/j.ebiom.2020.102740
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