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Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells

Vitamin D is associated with cardiovascular health through activating the vitamin D receptor that targets genes related to cardiovascular disease (CVD). The human cardiac microvascular endothelial cells (HCMECs) were used to develop mechanically and TGF-β1-induced fibrosis models, and the rat was us...

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Autores principales: Lai, Chi-Cheng, Juang, Wang-Chuan, Sun, Gwo-Ching, Tseng, Yu-Kai, Jhong, Rong-Chang, Tseng, Ching-Jiunn, Wong, Tzyy-Yue, Cheng, Pei-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139430/
https://www.ncbi.nlm.nih.gov/pubmed/32235811
http://dx.doi.org/10.3390/ijms21062196
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author Lai, Chi-Cheng
Juang, Wang-Chuan
Sun, Gwo-Ching
Tseng, Yu-Kai
Jhong, Rong-Chang
Tseng, Ching-Jiunn
Wong, Tzyy-Yue
Cheng, Pei-Wen
author_facet Lai, Chi-Cheng
Juang, Wang-Chuan
Sun, Gwo-Ching
Tseng, Yu-Kai
Jhong, Rong-Chang
Tseng, Ching-Jiunn
Wong, Tzyy-Yue
Cheng, Pei-Wen
author_sort Lai, Chi-Cheng
collection PubMed
description Vitamin D is associated with cardiovascular health through activating the vitamin D receptor that targets genes related to cardiovascular disease (CVD). The human cardiac microvascular endothelial cells (HCMECs) were used to develop mechanically and TGF-β1-induced fibrosis models, and the rat was used as the isoproterenol (ISO)-induced fibrosis model. The rats were injected with ISO for the first five days, followed by vitamin D injection for the consecutive three weeks before being sacrificed on the fourth week. Results showed that mechanical stretching reduced endothelial cell marker CD31 and VE-cadherin protein expressions, as well as increased α-smooth muscle actin (α-SMA) and fibronectin (FN). The transforming growth factor-β1 (TGF-β1) reduced CD31, and increased α-SMA and FN protein expression levels. Vitamin D presence led to higher protein expression of CD31, and lower protein expressions of α-SMA and FN compared to the control in the TGF-β1-induced fibrosis model. Additionally, protein expression of VE-cadherin was increased and fibroblast-specific protein-1 (FSP1) was decreased after vitamin D treatment in the ISO-induced fibrosis rat. In conclusion, vitamin D slightly inhibited fibrosis development in cell and animal models. Based on this study, the beneficial effect of vitamin D may be insignificant; however, further investigation of vitamin D’s effect in the long-term is required in the future.
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spelling pubmed-71394302020-04-10 Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells Lai, Chi-Cheng Juang, Wang-Chuan Sun, Gwo-Ching Tseng, Yu-Kai Jhong, Rong-Chang Tseng, Ching-Jiunn Wong, Tzyy-Yue Cheng, Pei-Wen Int J Mol Sci Article Vitamin D is associated with cardiovascular health through activating the vitamin D receptor that targets genes related to cardiovascular disease (CVD). The human cardiac microvascular endothelial cells (HCMECs) were used to develop mechanically and TGF-β1-induced fibrosis models, and the rat was used as the isoproterenol (ISO)-induced fibrosis model. The rats were injected with ISO for the first five days, followed by vitamin D injection for the consecutive three weeks before being sacrificed on the fourth week. Results showed that mechanical stretching reduced endothelial cell marker CD31 and VE-cadherin protein expressions, as well as increased α-smooth muscle actin (α-SMA) and fibronectin (FN). The transforming growth factor-β1 (TGF-β1) reduced CD31, and increased α-SMA and FN protein expression levels. Vitamin D presence led to higher protein expression of CD31, and lower protein expressions of α-SMA and FN compared to the control in the TGF-β1-induced fibrosis model. Additionally, protein expression of VE-cadherin was increased and fibroblast-specific protein-1 (FSP1) was decreased after vitamin D treatment in the ISO-induced fibrosis rat. In conclusion, vitamin D slightly inhibited fibrosis development in cell and animal models. Based on this study, the beneficial effect of vitamin D may be insignificant; however, further investigation of vitamin D’s effect in the long-term is required in the future. MDPI 2020-03-22 /pmc/articles/PMC7139430/ /pubmed/32235811 http://dx.doi.org/10.3390/ijms21062196 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lai, Chi-Cheng
Juang, Wang-Chuan
Sun, Gwo-Ching
Tseng, Yu-Kai
Jhong, Rong-Chang
Tseng, Ching-Jiunn
Wong, Tzyy-Yue
Cheng, Pei-Wen
Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells
title Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells
title_full Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells
title_fullStr Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells
title_full_unstemmed Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells
title_short Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells
title_sort vitamin d attenuates loss of endothelial biomarker expression in cardio-endothelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139430/
https://www.ncbi.nlm.nih.gov/pubmed/32235811
http://dx.doi.org/10.3390/ijms21062196
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