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Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells
Vitamin D is associated with cardiovascular health through activating the vitamin D receptor that targets genes related to cardiovascular disease (CVD). The human cardiac microvascular endothelial cells (HCMECs) were used to develop mechanically and TGF-β1-induced fibrosis models, and the rat was us...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139430/ https://www.ncbi.nlm.nih.gov/pubmed/32235811 http://dx.doi.org/10.3390/ijms21062196 |
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author | Lai, Chi-Cheng Juang, Wang-Chuan Sun, Gwo-Ching Tseng, Yu-Kai Jhong, Rong-Chang Tseng, Ching-Jiunn Wong, Tzyy-Yue Cheng, Pei-Wen |
author_facet | Lai, Chi-Cheng Juang, Wang-Chuan Sun, Gwo-Ching Tseng, Yu-Kai Jhong, Rong-Chang Tseng, Ching-Jiunn Wong, Tzyy-Yue Cheng, Pei-Wen |
author_sort | Lai, Chi-Cheng |
collection | PubMed |
description | Vitamin D is associated with cardiovascular health through activating the vitamin D receptor that targets genes related to cardiovascular disease (CVD). The human cardiac microvascular endothelial cells (HCMECs) were used to develop mechanically and TGF-β1-induced fibrosis models, and the rat was used as the isoproterenol (ISO)-induced fibrosis model. The rats were injected with ISO for the first five days, followed by vitamin D injection for the consecutive three weeks before being sacrificed on the fourth week. Results showed that mechanical stretching reduced endothelial cell marker CD31 and VE-cadherin protein expressions, as well as increased α-smooth muscle actin (α-SMA) and fibronectin (FN). The transforming growth factor-β1 (TGF-β1) reduced CD31, and increased α-SMA and FN protein expression levels. Vitamin D presence led to higher protein expression of CD31, and lower protein expressions of α-SMA and FN compared to the control in the TGF-β1-induced fibrosis model. Additionally, protein expression of VE-cadherin was increased and fibroblast-specific protein-1 (FSP1) was decreased after vitamin D treatment in the ISO-induced fibrosis rat. In conclusion, vitamin D slightly inhibited fibrosis development in cell and animal models. Based on this study, the beneficial effect of vitamin D may be insignificant; however, further investigation of vitamin D’s effect in the long-term is required in the future. |
format | Online Article Text |
id | pubmed-7139430 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71394302020-04-10 Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells Lai, Chi-Cheng Juang, Wang-Chuan Sun, Gwo-Ching Tseng, Yu-Kai Jhong, Rong-Chang Tseng, Ching-Jiunn Wong, Tzyy-Yue Cheng, Pei-Wen Int J Mol Sci Article Vitamin D is associated with cardiovascular health through activating the vitamin D receptor that targets genes related to cardiovascular disease (CVD). The human cardiac microvascular endothelial cells (HCMECs) were used to develop mechanically and TGF-β1-induced fibrosis models, and the rat was used as the isoproterenol (ISO)-induced fibrosis model. The rats were injected with ISO for the first five days, followed by vitamin D injection for the consecutive three weeks before being sacrificed on the fourth week. Results showed that mechanical stretching reduced endothelial cell marker CD31 and VE-cadherin protein expressions, as well as increased α-smooth muscle actin (α-SMA) and fibronectin (FN). The transforming growth factor-β1 (TGF-β1) reduced CD31, and increased α-SMA and FN protein expression levels. Vitamin D presence led to higher protein expression of CD31, and lower protein expressions of α-SMA and FN compared to the control in the TGF-β1-induced fibrosis model. Additionally, protein expression of VE-cadherin was increased and fibroblast-specific protein-1 (FSP1) was decreased after vitamin D treatment in the ISO-induced fibrosis rat. In conclusion, vitamin D slightly inhibited fibrosis development in cell and animal models. Based on this study, the beneficial effect of vitamin D may be insignificant; however, further investigation of vitamin D’s effect in the long-term is required in the future. MDPI 2020-03-22 /pmc/articles/PMC7139430/ /pubmed/32235811 http://dx.doi.org/10.3390/ijms21062196 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lai, Chi-Cheng Juang, Wang-Chuan Sun, Gwo-Ching Tseng, Yu-Kai Jhong, Rong-Chang Tseng, Ching-Jiunn Wong, Tzyy-Yue Cheng, Pei-Wen Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells |
title | Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells |
title_full | Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells |
title_fullStr | Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells |
title_full_unstemmed | Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells |
title_short | Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells |
title_sort | vitamin d attenuates loss of endothelial biomarker expression in cardio-endothelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139430/ https://www.ncbi.nlm.nih.gov/pubmed/32235811 http://dx.doi.org/10.3390/ijms21062196 |
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