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Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic
Background: Cellulite is a condition in which the skin has a dimpled lumpy appearance. The main causes of cellulite development, studied until now, comprehends modified sensitivity to estrogens, the damage of microvasculature present among dermis and hypodermis. The differences of adipose tissue arc...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139738/ https://www.ncbi.nlm.nih.gov/pubmed/32197394 http://dx.doi.org/10.3390/ijms21062077 |
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author | Conti, Giamaica Zingaretti, Nicola Amuso, Domenico Dai Prè, Elena Brandi, Jessica Cecconi, Daniela Manfredi, Marcello Marengo, Emilio Boschi, Federico Riccio, Michele Amore, Roberto Iorio, Eugenio Luigi Busato, Alice De Francesco, Francesco Riccio, Valentina Parodi, Pier Camillo Vaienti, Luca Sbarbati, Andrea |
author_facet | Conti, Giamaica Zingaretti, Nicola Amuso, Domenico Dai Prè, Elena Brandi, Jessica Cecconi, Daniela Manfredi, Marcello Marengo, Emilio Boschi, Federico Riccio, Michele Amore, Roberto Iorio, Eugenio Luigi Busato, Alice De Francesco, Francesco Riccio, Valentina Parodi, Pier Camillo Vaienti, Luca Sbarbati, Andrea |
author_sort | Conti, Giamaica |
collection | PubMed |
description | Background: Cellulite is a condition in which the skin has a dimpled lumpy appearance. The main causes of cellulite development, studied until now, comprehends modified sensitivity to estrogens, the damage of microvasculature present among dermis and hypodermis. The differences of adipose tissue architecture between male and female might make female more susceptible to cellulite. Adipose tissue is seen to be deeply modified during cellulite development. Our study tried to understand the overall features within and surrounding cellulite to apply the best therapeutic approach. Methods: Samples of gluteal femoral area were collected from cadavers and women who had undergone surgical treatment to remove orange peel characteristics on the skin. Samples from cadavers were employed for an accurate study of cellulite using magnetic resonance imaging at 7 Tesla and for light microscopy. Specimens from patients were employed for the proteomic analysis, which was performed using high resolution mass spectroscopy (MS). Stromal vascular fraction (SVF) was obtained from the samples, which was studied using MS and flow cytometry. Results: light and electron microscopy of the cellulite affected area showed a morphology completely different from the other usual adipose depots. In cellulite affected tissues, sweat glands associated with adipocytes were found. In particular, there were vesicles in the extracellular matrix, indicating a crosstalk between the two different components. Proteomic analysis showed that adipose tissue affected by cellulite is characterized by high degree of oxidative stress and by remodeling phenomena. Conclusions: The novel aspects of this study are the peculiar morphology of adipose tissue affected by cellulite, which could influence the surgical procedures finalized to the reduction of dimpling, based on the collagen fibers cutting. The second novel aspect is the role played by the mesenchymal stem cells isolated from stromal vascular fraction of adipose tissue affected by cellulite. |
format | Online Article Text |
id | pubmed-7139738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71397382020-04-10 Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic Conti, Giamaica Zingaretti, Nicola Amuso, Domenico Dai Prè, Elena Brandi, Jessica Cecconi, Daniela Manfredi, Marcello Marengo, Emilio Boschi, Federico Riccio, Michele Amore, Roberto Iorio, Eugenio Luigi Busato, Alice De Francesco, Francesco Riccio, Valentina Parodi, Pier Camillo Vaienti, Luca Sbarbati, Andrea Int J Mol Sci Article Background: Cellulite is a condition in which the skin has a dimpled lumpy appearance. The main causes of cellulite development, studied until now, comprehends modified sensitivity to estrogens, the damage of microvasculature present among dermis and hypodermis. The differences of adipose tissue architecture between male and female might make female more susceptible to cellulite. Adipose tissue is seen to be deeply modified during cellulite development. Our study tried to understand the overall features within and surrounding cellulite to apply the best therapeutic approach. Methods: Samples of gluteal femoral area were collected from cadavers and women who had undergone surgical treatment to remove orange peel characteristics on the skin. Samples from cadavers were employed for an accurate study of cellulite using magnetic resonance imaging at 7 Tesla and for light microscopy. Specimens from patients were employed for the proteomic analysis, which was performed using high resolution mass spectroscopy (MS). Stromal vascular fraction (SVF) was obtained from the samples, which was studied using MS and flow cytometry. Results: light and electron microscopy of the cellulite affected area showed a morphology completely different from the other usual adipose depots. In cellulite affected tissues, sweat glands associated with adipocytes were found. In particular, there were vesicles in the extracellular matrix, indicating a crosstalk between the two different components. Proteomic analysis showed that adipose tissue affected by cellulite is characterized by high degree of oxidative stress and by remodeling phenomena. Conclusions: The novel aspects of this study are the peculiar morphology of adipose tissue affected by cellulite, which could influence the surgical procedures finalized to the reduction of dimpling, based on the collagen fibers cutting. The second novel aspect is the role played by the mesenchymal stem cells isolated from stromal vascular fraction of adipose tissue affected by cellulite. MDPI 2020-03-18 /pmc/articles/PMC7139738/ /pubmed/32197394 http://dx.doi.org/10.3390/ijms21062077 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Conti, Giamaica Zingaretti, Nicola Amuso, Domenico Dai Prè, Elena Brandi, Jessica Cecconi, Daniela Manfredi, Marcello Marengo, Emilio Boschi, Federico Riccio, Michele Amore, Roberto Iorio, Eugenio Luigi Busato, Alice De Francesco, Francesco Riccio, Valentina Parodi, Pier Camillo Vaienti, Luca Sbarbati, Andrea Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic |
title | Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic |
title_full | Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic |
title_fullStr | Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic |
title_full_unstemmed | Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic |
title_short | Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic |
title_sort | proteomic and ultrastructural analysis of cellulite—new findings on an old topic |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139738/ https://www.ncbi.nlm.nih.gov/pubmed/32197394 http://dx.doi.org/10.3390/ijms21062077 |
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