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Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic

Background: Cellulite is a condition in which the skin has a dimpled lumpy appearance. The main causes of cellulite development, studied until now, comprehends modified sensitivity to estrogens, the damage of microvasculature present among dermis and hypodermis. The differences of adipose tissue arc...

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Autores principales: Conti, Giamaica, Zingaretti, Nicola, Amuso, Domenico, Dai Prè, Elena, Brandi, Jessica, Cecconi, Daniela, Manfredi, Marcello, Marengo, Emilio, Boschi, Federico, Riccio, Michele, Amore, Roberto, Iorio, Eugenio Luigi, Busato, Alice, De Francesco, Francesco, Riccio, Valentina, Parodi, Pier Camillo, Vaienti, Luca, Sbarbati, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139738/
https://www.ncbi.nlm.nih.gov/pubmed/32197394
http://dx.doi.org/10.3390/ijms21062077
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author Conti, Giamaica
Zingaretti, Nicola
Amuso, Domenico
Dai Prè, Elena
Brandi, Jessica
Cecconi, Daniela
Manfredi, Marcello
Marengo, Emilio
Boschi, Federico
Riccio, Michele
Amore, Roberto
Iorio, Eugenio Luigi
Busato, Alice
De Francesco, Francesco
Riccio, Valentina
Parodi, Pier Camillo
Vaienti, Luca
Sbarbati, Andrea
author_facet Conti, Giamaica
Zingaretti, Nicola
Amuso, Domenico
Dai Prè, Elena
Brandi, Jessica
Cecconi, Daniela
Manfredi, Marcello
Marengo, Emilio
Boschi, Federico
Riccio, Michele
Amore, Roberto
Iorio, Eugenio Luigi
Busato, Alice
De Francesco, Francesco
Riccio, Valentina
Parodi, Pier Camillo
Vaienti, Luca
Sbarbati, Andrea
author_sort Conti, Giamaica
collection PubMed
description Background: Cellulite is a condition in which the skin has a dimpled lumpy appearance. The main causes of cellulite development, studied until now, comprehends modified sensitivity to estrogens, the damage of microvasculature present among dermis and hypodermis. The differences of adipose tissue architecture between male and female might make female more susceptible to cellulite. Adipose tissue is seen to be deeply modified during cellulite development. Our study tried to understand the overall features within and surrounding cellulite to apply the best therapeutic approach. Methods: Samples of gluteal femoral area were collected from cadavers and women who had undergone surgical treatment to remove orange peel characteristics on the skin. Samples from cadavers were employed for an accurate study of cellulite using magnetic resonance imaging at 7 Tesla and for light microscopy. Specimens from patients were employed for the proteomic analysis, which was performed using high resolution mass spectroscopy (MS). Stromal vascular fraction (SVF) was obtained from the samples, which was studied using MS and flow cytometry. Results: light and electron microscopy of the cellulite affected area showed a morphology completely different from the other usual adipose depots. In cellulite affected tissues, sweat glands associated with adipocytes were found. In particular, there were vesicles in the extracellular matrix, indicating a crosstalk between the two different components. Proteomic analysis showed that adipose tissue affected by cellulite is characterized by high degree of oxidative stress and by remodeling phenomena. Conclusions: The novel aspects of this study are the peculiar morphology of adipose tissue affected by cellulite, which could influence the surgical procedures finalized to the reduction of dimpling, based on the collagen fibers cutting. The second novel aspect is the role played by the mesenchymal stem cells isolated from stromal vascular fraction of adipose tissue affected by cellulite.
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spelling pubmed-71397382020-04-10 Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic Conti, Giamaica Zingaretti, Nicola Amuso, Domenico Dai Prè, Elena Brandi, Jessica Cecconi, Daniela Manfredi, Marcello Marengo, Emilio Boschi, Federico Riccio, Michele Amore, Roberto Iorio, Eugenio Luigi Busato, Alice De Francesco, Francesco Riccio, Valentina Parodi, Pier Camillo Vaienti, Luca Sbarbati, Andrea Int J Mol Sci Article Background: Cellulite is a condition in which the skin has a dimpled lumpy appearance. The main causes of cellulite development, studied until now, comprehends modified sensitivity to estrogens, the damage of microvasculature present among dermis and hypodermis. The differences of adipose tissue architecture between male and female might make female more susceptible to cellulite. Adipose tissue is seen to be deeply modified during cellulite development. Our study tried to understand the overall features within and surrounding cellulite to apply the best therapeutic approach. Methods: Samples of gluteal femoral area were collected from cadavers and women who had undergone surgical treatment to remove orange peel characteristics on the skin. Samples from cadavers were employed for an accurate study of cellulite using magnetic resonance imaging at 7 Tesla and for light microscopy. Specimens from patients were employed for the proteomic analysis, which was performed using high resolution mass spectroscopy (MS). Stromal vascular fraction (SVF) was obtained from the samples, which was studied using MS and flow cytometry. Results: light and electron microscopy of the cellulite affected area showed a morphology completely different from the other usual adipose depots. In cellulite affected tissues, sweat glands associated with adipocytes were found. In particular, there were vesicles in the extracellular matrix, indicating a crosstalk between the two different components. Proteomic analysis showed that adipose tissue affected by cellulite is characterized by high degree of oxidative stress and by remodeling phenomena. Conclusions: The novel aspects of this study are the peculiar morphology of adipose tissue affected by cellulite, which could influence the surgical procedures finalized to the reduction of dimpling, based on the collagen fibers cutting. The second novel aspect is the role played by the mesenchymal stem cells isolated from stromal vascular fraction of adipose tissue affected by cellulite. MDPI 2020-03-18 /pmc/articles/PMC7139738/ /pubmed/32197394 http://dx.doi.org/10.3390/ijms21062077 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Conti, Giamaica
Zingaretti, Nicola
Amuso, Domenico
Dai Prè, Elena
Brandi, Jessica
Cecconi, Daniela
Manfredi, Marcello
Marengo, Emilio
Boschi, Federico
Riccio, Michele
Amore, Roberto
Iorio, Eugenio Luigi
Busato, Alice
De Francesco, Francesco
Riccio, Valentina
Parodi, Pier Camillo
Vaienti, Luca
Sbarbati, Andrea
Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic
title Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic
title_full Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic
title_fullStr Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic
title_full_unstemmed Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic
title_short Proteomic and Ultrastructural Analysis of Cellulite—New Findings on an Old Topic
title_sort proteomic and ultrastructural analysis of cellulite—new findings on an old topic
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139738/
https://www.ncbi.nlm.nih.gov/pubmed/32197394
http://dx.doi.org/10.3390/ijms21062077
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