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Role of Microtubule-Associated Protein 1b in Urothelial Carcinoma: Overexpression Predicts Poor Prognosis
We sought to examine the relationship between microtubule-associated proteins (MAPs) and the prognosis of urothelial carcinoma by assessing the microtubule bundle formation genes using a reappraisal transcriptome dataset of urothelial carcinoma (GSE31684). The result revealed that microtubule-associ...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139768/ https://www.ncbi.nlm.nih.gov/pubmed/32182788 http://dx.doi.org/10.3390/cancers12030630 |
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author | Chien, Tsu-Ming Chan, Ti-Chun Huang, Steven Kuan-Hua Yeh, Bi-Wen Li, Wei-Ming Huang, Chun-Nung Li, Ching-Chia Wu, Wen-Jeng Li, Chien-Feng |
author_facet | Chien, Tsu-Ming Chan, Ti-Chun Huang, Steven Kuan-Hua Yeh, Bi-Wen Li, Wei-Ming Huang, Chun-Nung Li, Ching-Chia Wu, Wen-Jeng Li, Chien-Feng |
author_sort | Chien, Tsu-Ming |
collection | PubMed |
description | We sought to examine the relationship between microtubule-associated proteins (MAPs) and the prognosis of urothelial carcinoma by assessing the microtubule bundle formation genes using a reappraisal transcriptome dataset of urothelial carcinoma (GSE31684). The result revealed that microtubule-associated protein 1b (MAP1B) is the most significant upregulated gene related to cancer progression. Real-time reverse-transcription polymerase chain reaction was used to measure MAP1B transcription levels in urothelial carcinoma of the upper tract (UTUC) and the bladder (UBUC). Immunohistochemistry was conducted to detect MAP1B protein expression in 340 UTUC and 295 UBUC cases. Correlations of MAP1B expression with clinicopathological status, disease-specific survival, and metastasis-free survival were completed. To assess the oncogenic functions of MAP1B, the RTCC1 and J82 cell lines were stably silenced against their endogenous MAP1B expression. Study findings indicated that MAP1B overexpression was associated with adverse clinical features and could independently predict unfavorable prognostic effects, indicating its theranostic value in urothelial carcinoma. |
format | Online Article Text |
id | pubmed-7139768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71397682020-04-10 Role of Microtubule-Associated Protein 1b in Urothelial Carcinoma: Overexpression Predicts Poor Prognosis Chien, Tsu-Ming Chan, Ti-Chun Huang, Steven Kuan-Hua Yeh, Bi-Wen Li, Wei-Ming Huang, Chun-Nung Li, Ching-Chia Wu, Wen-Jeng Li, Chien-Feng Cancers (Basel) Article We sought to examine the relationship between microtubule-associated proteins (MAPs) and the prognosis of urothelial carcinoma by assessing the microtubule bundle formation genes using a reappraisal transcriptome dataset of urothelial carcinoma (GSE31684). The result revealed that microtubule-associated protein 1b (MAP1B) is the most significant upregulated gene related to cancer progression. Real-time reverse-transcription polymerase chain reaction was used to measure MAP1B transcription levels in urothelial carcinoma of the upper tract (UTUC) and the bladder (UBUC). Immunohistochemistry was conducted to detect MAP1B protein expression in 340 UTUC and 295 UBUC cases. Correlations of MAP1B expression with clinicopathological status, disease-specific survival, and metastasis-free survival were completed. To assess the oncogenic functions of MAP1B, the RTCC1 and J82 cell lines were stably silenced against their endogenous MAP1B expression. Study findings indicated that MAP1B overexpression was associated with adverse clinical features and could independently predict unfavorable prognostic effects, indicating its theranostic value in urothelial carcinoma. MDPI 2020-03-09 /pmc/articles/PMC7139768/ /pubmed/32182788 http://dx.doi.org/10.3390/cancers12030630 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chien, Tsu-Ming Chan, Ti-Chun Huang, Steven Kuan-Hua Yeh, Bi-Wen Li, Wei-Ming Huang, Chun-Nung Li, Ching-Chia Wu, Wen-Jeng Li, Chien-Feng Role of Microtubule-Associated Protein 1b in Urothelial Carcinoma: Overexpression Predicts Poor Prognosis |
title | Role of Microtubule-Associated Protein 1b in Urothelial Carcinoma: Overexpression Predicts Poor Prognosis |
title_full | Role of Microtubule-Associated Protein 1b in Urothelial Carcinoma: Overexpression Predicts Poor Prognosis |
title_fullStr | Role of Microtubule-Associated Protein 1b in Urothelial Carcinoma: Overexpression Predicts Poor Prognosis |
title_full_unstemmed | Role of Microtubule-Associated Protein 1b in Urothelial Carcinoma: Overexpression Predicts Poor Prognosis |
title_short | Role of Microtubule-Associated Protein 1b in Urothelial Carcinoma: Overexpression Predicts Poor Prognosis |
title_sort | role of microtubule-associated protein 1b in urothelial carcinoma: overexpression predicts poor prognosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139768/ https://www.ncbi.nlm.nih.gov/pubmed/32182788 http://dx.doi.org/10.3390/cancers12030630 |
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