Cargando…

Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea

Background: Epigenetic changes in obstructive sleep apnea (OSA) have been proposed as a mechanism for end-organ vulnerability. In children with OSA, Forkhead Box P3 (FOXP3) DNA methylation were associated with inflammatory biomarkers; however, the methylation pattern and its effect in the expression...

Descripción completa

Detalles Bibliográficos
Autores principales: Sanz-Rubio, David, Sanz, Arianne, Varona, Luis, Bolea, Rosa, Forner, Marta, Gil, Ana V., Cubero, Pablo, Marin-Oto, Marta, Martin-Burriel, Inmaculada, Marin, Jose M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139835/
https://www.ncbi.nlm.nih.gov/pubmed/32210181
http://dx.doi.org/10.3390/ijms21062233
_version_ 1783518858072555520
author Sanz-Rubio, David
Sanz, Arianne
Varona, Luis
Bolea, Rosa
Forner, Marta
Gil, Ana V.
Cubero, Pablo
Marin-Oto, Marta
Martin-Burriel, Inmaculada
Marin, Jose M.
author_facet Sanz-Rubio, David
Sanz, Arianne
Varona, Luis
Bolea, Rosa
Forner, Marta
Gil, Ana V.
Cubero, Pablo
Marin-Oto, Marta
Martin-Burriel, Inmaculada
Marin, Jose M.
author_sort Sanz-Rubio, David
collection PubMed
description Background: Epigenetic changes in obstructive sleep apnea (OSA) have been proposed as a mechanism for end-organ vulnerability. In children with OSA, Forkhead Box P3 (FOXP3) DNA methylation were associated with inflammatory biomarkers; however, the methylation pattern and its effect in the expression of this gene have not been tested in adults with OSA. Methods: Plasma samples from subjects without comorbid conditions other than OSA were analyzed (the Epigenetics Status and Subclinical Atherosclerosis in Obstructive Sleep Apnea (EPIOSA) Study: NCT02131610). In 16 patients with severe OSA (Apnea-Hypopnea Index—AHI- > 30 events/h) and seven matched controls (AHI < 5), methylation of FOXP3 gen was evaluated by PCR of the promoter and by pyrosequencing of the intron 1 Treg-specific demethylated region (TSDR). In another 74 patients with OSA (AHI > 10) and 31 controls, we quantified FOXP3 protein expression by ELISA and gene expression by quantitative real-time PCR. C-reactive protein (CRP) and plasma Treg cells were also evaluated. Results: Neither the levels of the promoter nor the TSDR demethylated region were different between controls and patients with OSA, whether they were grouped by normal or high CRP. FOXP3 protein and mRNA expression did not differ between groups. Conclusions: FOXP3 methylation or its expression is not altered in adults with OSA, whatever their inflammatory status.
format Online
Article
Text
id pubmed-7139835
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71398352020-04-10 Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea Sanz-Rubio, David Sanz, Arianne Varona, Luis Bolea, Rosa Forner, Marta Gil, Ana V. Cubero, Pablo Marin-Oto, Marta Martin-Burriel, Inmaculada Marin, Jose M. Int J Mol Sci Article Background: Epigenetic changes in obstructive sleep apnea (OSA) have been proposed as a mechanism for end-organ vulnerability. In children with OSA, Forkhead Box P3 (FOXP3) DNA methylation were associated with inflammatory biomarkers; however, the methylation pattern and its effect in the expression of this gene have not been tested in adults with OSA. Methods: Plasma samples from subjects without comorbid conditions other than OSA were analyzed (the Epigenetics Status and Subclinical Atherosclerosis in Obstructive Sleep Apnea (EPIOSA) Study: NCT02131610). In 16 patients with severe OSA (Apnea-Hypopnea Index—AHI- > 30 events/h) and seven matched controls (AHI < 5), methylation of FOXP3 gen was evaluated by PCR of the promoter and by pyrosequencing of the intron 1 Treg-specific demethylated region (TSDR). In another 74 patients with OSA (AHI > 10) and 31 controls, we quantified FOXP3 protein expression by ELISA and gene expression by quantitative real-time PCR. C-reactive protein (CRP) and plasma Treg cells were also evaluated. Results: Neither the levels of the promoter nor the TSDR demethylated region were different between controls and patients with OSA, whether they were grouped by normal or high CRP. FOXP3 protein and mRNA expression did not differ between groups. Conclusions: FOXP3 methylation or its expression is not altered in adults with OSA, whatever their inflammatory status. MDPI 2020-03-23 /pmc/articles/PMC7139835/ /pubmed/32210181 http://dx.doi.org/10.3390/ijms21062233 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sanz-Rubio, David
Sanz, Arianne
Varona, Luis
Bolea, Rosa
Forner, Marta
Gil, Ana V.
Cubero, Pablo
Marin-Oto, Marta
Martin-Burriel, Inmaculada
Marin, Jose M.
Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea
title Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea
title_full Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea
title_fullStr Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea
title_full_unstemmed Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea
title_short Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea
title_sort forkhead box p3 methylation and expression in men with obstructive sleep apnea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139835/
https://www.ncbi.nlm.nih.gov/pubmed/32210181
http://dx.doi.org/10.3390/ijms21062233
work_keys_str_mv AT sanzrubiodavid forkheadboxp3methylationandexpressioninmenwithobstructivesleepapnea
AT sanzarianne forkheadboxp3methylationandexpressioninmenwithobstructivesleepapnea
AT varonaluis forkheadboxp3methylationandexpressioninmenwithobstructivesleepapnea
AT bolearosa forkheadboxp3methylationandexpressioninmenwithobstructivesleepapnea
AT fornermarta forkheadboxp3methylationandexpressioninmenwithobstructivesleepapnea
AT gilanav forkheadboxp3methylationandexpressioninmenwithobstructivesleepapnea
AT cuberopablo forkheadboxp3methylationandexpressioninmenwithobstructivesleepapnea
AT marinotomarta forkheadboxp3methylationandexpressioninmenwithobstructivesleepapnea
AT martinburrielinmaculada forkheadboxp3methylationandexpressioninmenwithobstructivesleepapnea
AT marinjosem forkheadboxp3methylationandexpressioninmenwithobstructivesleepapnea