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In Vitro and In Vivo Nutraceutical Characterization of Two Chickpea Accessions: Differential Effects on Hepatic Lipid Over-Accumulation

Dietary habits are crucially important to prevent the development of lifestyle-associated diseases. Diets supplemented with chickpeas have numerous benefits and are known to improve body fat composition. The present study was undertaken to characterize two genetically and phenotypically distinct acc...

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Autores principales: Centrone, Mariangela, Gena, Patrizia, Ranieri, Marianna, Di Mise, Annarita, D’Agostino, Mariagrazia, Mastrodonato, Maria, Venneri, Maria, De Angelis, Davide, Pavan, Stefano, Pasqualone, Antonella, Summo, Carmine, Fanelli, Valentina, Valenti, Giovanna, Calamita, Giuseppe, Tamma, Grazia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139887/
https://www.ncbi.nlm.nih.gov/pubmed/32214012
http://dx.doi.org/10.3390/antiox9030268
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author Centrone, Mariangela
Gena, Patrizia
Ranieri, Marianna
Di Mise, Annarita
D’Agostino, Mariagrazia
Mastrodonato, Maria
Venneri, Maria
De Angelis, Davide
Pavan, Stefano
Pasqualone, Antonella
Summo, Carmine
Fanelli, Valentina
Valenti, Giovanna
Calamita, Giuseppe
Tamma, Grazia
author_facet Centrone, Mariangela
Gena, Patrizia
Ranieri, Marianna
Di Mise, Annarita
D’Agostino, Mariagrazia
Mastrodonato, Maria
Venneri, Maria
De Angelis, Davide
Pavan, Stefano
Pasqualone, Antonella
Summo, Carmine
Fanelli, Valentina
Valenti, Giovanna
Calamita, Giuseppe
Tamma, Grazia
author_sort Centrone, Mariangela
collection PubMed
description Dietary habits are crucially important to prevent the development of lifestyle-associated diseases. Diets supplemented with chickpeas have numerous benefits and are known to improve body fat composition. The present study was undertaken to characterize two genetically and phenotypically distinct accessions, MG_13 and PI358934, selected from a global chickpea collection. Rat hepatoma FaO cells treated with a mixture of free fatty acids (FFAs) (O/P) were used as an in vitro model of hepatic steatosis. In parallel, a high-fat diet (HFD) animal model was also established. In vitro and in vivo studies revealed that both chickpea accessions showed a significant antioxidant ability. However, only MG_13 reduced the lipid over-accumulation in steatotic FaO cells and in the liver of HFD fed mice. Moreover, mice fed with HFD + MG_13 displayed a lower level of glycemia and aspartate aminotransferase (AST) than HFD mice. Interestingly, exposure to MG_13 prevented the phosphorylation of the inflammatory nuclear factor kappa beta (NF-kB) which is upregulated during HFD and known to be linked to obesity. To conclude, the comparison of the two distinct chickpea accessions revealed a beneficial effect only for the MG_13. These findings highlight the importance of studies addressing the functional characterization of chickpea biodiversity and nutraceutical properties.
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spelling pubmed-71398872020-04-13 In Vitro and In Vivo Nutraceutical Characterization of Two Chickpea Accessions: Differential Effects on Hepatic Lipid Over-Accumulation Centrone, Mariangela Gena, Patrizia Ranieri, Marianna Di Mise, Annarita D’Agostino, Mariagrazia Mastrodonato, Maria Venneri, Maria De Angelis, Davide Pavan, Stefano Pasqualone, Antonella Summo, Carmine Fanelli, Valentina Valenti, Giovanna Calamita, Giuseppe Tamma, Grazia Antioxidants (Basel) Article Dietary habits are crucially important to prevent the development of lifestyle-associated diseases. Diets supplemented with chickpeas have numerous benefits and are known to improve body fat composition. The present study was undertaken to characterize two genetically and phenotypically distinct accessions, MG_13 and PI358934, selected from a global chickpea collection. Rat hepatoma FaO cells treated with a mixture of free fatty acids (FFAs) (O/P) were used as an in vitro model of hepatic steatosis. In parallel, a high-fat diet (HFD) animal model was also established. In vitro and in vivo studies revealed that both chickpea accessions showed a significant antioxidant ability. However, only MG_13 reduced the lipid over-accumulation in steatotic FaO cells and in the liver of HFD fed mice. Moreover, mice fed with HFD + MG_13 displayed a lower level of glycemia and aspartate aminotransferase (AST) than HFD mice. Interestingly, exposure to MG_13 prevented the phosphorylation of the inflammatory nuclear factor kappa beta (NF-kB) which is upregulated during HFD and known to be linked to obesity. To conclude, the comparison of the two distinct chickpea accessions revealed a beneficial effect only for the MG_13. These findings highlight the importance of studies addressing the functional characterization of chickpea biodiversity and nutraceutical properties. MDPI 2020-03-24 /pmc/articles/PMC7139887/ /pubmed/32214012 http://dx.doi.org/10.3390/antiox9030268 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Centrone, Mariangela
Gena, Patrizia
Ranieri, Marianna
Di Mise, Annarita
D’Agostino, Mariagrazia
Mastrodonato, Maria
Venneri, Maria
De Angelis, Davide
Pavan, Stefano
Pasqualone, Antonella
Summo, Carmine
Fanelli, Valentina
Valenti, Giovanna
Calamita, Giuseppe
Tamma, Grazia
In Vitro and In Vivo Nutraceutical Characterization of Two Chickpea Accessions: Differential Effects on Hepatic Lipid Over-Accumulation
title In Vitro and In Vivo Nutraceutical Characterization of Two Chickpea Accessions: Differential Effects on Hepatic Lipid Over-Accumulation
title_full In Vitro and In Vivo Nutraceutical Characterization of Two Chickpea Accessions: Differential Effects on Hepatic Lipid Over-Accumulation
title_fullStr In Vitro and In Vivo Nutraceutical Characterization of Two Chickpea Accessions: Differential Effects on Hepatic Lipid Over-Accumulation
title_full_unstemmed In Vitro and In Vivo Nutraceutical Characterization of Two Chickpea Accessions: Differential Effects on Hepatic Lipid Over-Accumulation
title_short In Vitro and In Vivo Nutraceutical Characterization of Two Chickpea Accessions: Differential Effects on Hepatic Lipid Over-Accumulation
title_sort in vitro and in vivo nutraceutical characterization of two chickpea accessions: differential effects on hepatic lipid over-accumulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139887/
https://www.ncbi.nlm.nih.gov/pubmed/32214012
http://dx.doi.org/10.3390/antiox9030268
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