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Identification of Candidate Genes Involved in Curd Riceyness in Cauliflower

“Riceyness” refers to the precocious development of flower bud initials over the curd surface of cauliflower, and it is regarded as undesirable for the market. The present study aimed to identify the candidate loci and genes responsible for the morphological difference in riceyness between a pair of...

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Autores principales: Zhao, Zhenqing, Sheng, Xiaoguang, Yu, Huifang, Wang, Jiansheng, Shen, Yusen, Gu, Honghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139996/
https://www.ncbi.nlm.nih.gov/pubmed/32183438
http://dx.doi.org/10.3390/ijms21061999
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author Zhao, Zhenqing
Sheng, Xiaoguang
Yu, Huifang
Wang, Jiansheng
Shen, Yusen
Gu, Honghui
author_facet Zhao, Zhenqing
Sheng, Xiaoguang
Yu, Huifang
Wang, Jiansheng
Shen, Yusen
Gu, Honghui
author_sort Zhao, Zhenqing
collection PubMed
description “Riceyness” refers to the precocious development of flower bud initials over the curd surface of cauliflower, and it is regarded as undesirable for the market. The present study aimed to identify the candidate loci and genes responsible for the morphological difference in riceyness between a pair of cauliflower sister lines. Genetic analysis revealed that riceyness is controlled by a single dominant locus. An F(2) population derived from the cross between these sister lines was used to construct “riceyness” and “non-riceyness” bulks, and then it was subjected to BSA-seq. On the basis of the results of Δ(SNP-index) analysis, a 4.0 Mb candidate region including 22 putative SNPs was mapped on chromosome C04. Combining the RNA-seq, gene function annotation, and target sequence analysis among two parents and other breeding lines, an orthologous gene of the Arabidopsis gene SOC1, Bo4g024850 was presumed as the candidate gene, and an upstream SNP likely resulted in riceyness phenotype via influencing the expression levels of Bo4g024850. These results are helpful to understand the genetic mechanism regulating riceyness, and to facilitate the molecular improvement on cauliflower curds.
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spelling pubmed-71399962020-04-13 Identification of Candidate Genes Involved in Curd Riceyness in Cauliflower Zhao, Zhenqing Sheng, Xiaoguang Yu, Huifang Wang, Jiansheng Shen, Yusen Gu, Honghui Int J Mol Sci Article “Riceyness” refers to the precocious development of flower bud initials over the curd surface of cauliflower, and it is regarded as undesirable for the market. The present study aimed to identify the candidate loci and genes responsible for the morphological difference in riceyness between a pair of cauliflower sister lines. Genetic analysis revealed that riceyness is controlled by a single dominant locus. An F(2) population derived from the cross between these sister lines was used to construct “riceyness” and “non-riceyness” bulks, and then it was subjected to BSA-seq. On the basis of the results of Δ(SNP-index) analysis, a 4.0 Mb candidate region including 22 putative SNPs was mapped on chromosome C04. Combining the RNA-seq, gene function annotation, and target sequence analysis among two parents and other breeding lines, an orthologous gene of the Arabidopsis gene SOC1, Bo4g024850 was presumed as the candidate gene, and an upstream SNP likely resulted in riceyness phenotype via influencing the expression levels of Bo4g024850. These results are helpful to understand the genetic mechanism regulating riceyness, and to facilitate the molecular improvement on cauliflower curds. MDPI 2020-03-15 /pmc/articles/PMC7139996/ /pubmed/32183438 http://dx.doi.org/10.3390/ijms21061999 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Zhenqing
Sheng, Xiaoguang
Yu, Huifang
Wang, Jiansheng
Shen, Yusen
Gu, Honghui
Identification of Candidate Genes Involved in Curd Riceyness in Cauliflower
title Identification of Candidate Genes Involved in Curd Riceyness in Cauliflower
title_full Identification of Candidate Genes Involved in Curd Riceyness in Cauliflower
title_fullStr Identification of Candidate Genes Involved in Curd Riceyness in Cauliflower
title_full_unstemmed Identification of Candidate Genes Involved in Curd Riceyness in Cauliflower
title_short Identification of Candidate Genes Involved in Curd Riceyness in Cauliflower
title_sort identification of candidate genes involved in curd riceyness in cauliflower
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139996/
https://www.ncbi.nlm.nih.gov/pubmed/32183438
http://dx.doi.org/10.3390/ijms21061999
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