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Establishment and Molecular Phenotyping of Organoids from the Squamocolumnar Junction Region of the Uterine Cervix
The metaplastic epithelium of the transformation zone (TZ) including the squamocolumnar junction (SCJ) of the uterine cervix is a prime target of human papilloma virus (HPV) infection and subsequent cancer development. Due to the lack of adequate in vitro models for SCJ, however, investigations into...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140077/ https://www.ncbi.nlm.nih.gov/pubmed/32183493 http://dx.doi.org/10.3390/cancers12030694 |
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author | Maru, Yoshiaki Kawata, Akira Taguchi, Ayumi Ishii, Yoshiyuki Baba, Satoshi Mori, Mayuyo Nagamatsu, Takeshi Oda, Katsutoshi Kukimoto, Iwao Osuga, Yutaka Fujii, Tomoyuki Hippo, Yoshitaka |
author_facet | Maru, Yoshiaki Kawata, Akira Taguchi, Ayumi Ishii, Yoshiyuki Baba, Satoshi Mori, Mayuyo Nagamatsu, Takeshi Oda, Katsutoshi Kukimoto, Iwao Osuga, Yutaka Fujii, Tomoyuki Hippo, Yoshitaka |
author_sort | Maru, Yoshiaki |
collection | PubMed |
description | The metaplastic epithelium of the transformation zone (TZ) including the squamocolumnar junction (SCJ) of the uterine cervix is a prime target of human papilloma virus (HPV) infection and subsequent cancer development. Due to the lack of adequate in vitro models for SCJ, however, investigations into its physiological roles and vulnerability to carcinogenesis have been limited. By using Matrigel-based three-dimensional culture techniques, we propagated organoids derived from the normal SCJ region, along with metaplastic squamous cells in the TZ. Consisting predominantly of squamous cells, organoids basically exhibited a dense structure. However, at least in some organoids, a small but discrete population of mucin-producing endocervix cells co-existed adjacent to the squamous cell population, virtually recapitulating the configuration of SCJ in a TZ background. In addition, transcriptome analysis confirmed a higher expression level of many SCJ marker genes in organoids, compared to that in the immortalized cervical cell lines of non-SCJ origin. Thus, the obtained organoids appear to mimic cervical SCJ cells and, in particular, metaplastic squamous cells from the TZ, likely providing a novel platform in which HPV-driven cervical cancer development could be investigated. |
format | Online Article Text |
id | pubmed-7140077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71400772020-04-13 Establishment and Molecular Phenotyping of Organoids from the Squamocolumnar Junction Region of the Uterine Cervix Maru, Yoshiaki Kawata, Akira Taguchi, Ayumi Ishii, Yoshiyuki Baba, Satoshi Mori, Mayuyo Nagamatsu, Takeshi Oda, Katsutoshi Kukimoto, Iwao Osuga, Yutaka Fujii, Tomoyuki Hippo, Yoshitaka Cancers (Basel) Article The metaplastic epithelium of the transformation zone (TZ) including the squamocolumnar junction (SCJ) of the uterine cervix is a prime target of human papilloma virus (HPV) infection and subsequent cancer development. Due to the lack of adequate in vitro models for SCJ, however, investigations into its physiological roles and vulnerability to carcinogenesis have been limited. By using Matrigel-based three-dimensional culture techniques, we propagated organoids derived from the normal SCJ region, along with metaplastic squamous cells in the TZ. Consisting predominantly of squamous cells, organoids basically exhibited a dense structure. However, at least in some organoids, a small but discrete population of mucin-producing endocervix cells co-existed adjacent to the squamous cell population, virtually recapitulating the configuration of SCJ in a TZ background. In addition, transcriptome analysis confirmed a higher expression level of many SCJ marker genes in organoids, compared to that in the immortalized cervical cell lines of non-SCJ origin. Thus, the obtained organoids appear to mimic cervical SCJ cells and, in particular, metaplastic squamous cells from the TZ, likely providing a novel platform in which HPV-driven cervical cancer development could be investigated. MDPI 2020-03-15 /pmc/articles/PMC7140077/ /pubmed/32183493 http://dx.doi.org/10.3390/cancers12030694 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Maru, Yoshiaki Kawata, Akira Taguchi, Ayumi Ishii, Yoshiyuki Baba, Satoshi Mori, Mayuyo Nagamatsu, Takeshi Oda, Katsutoshi Kukimoto, Iwao Osuga, Yutaka Fujii, Tomoyuki Hippo, Yoshitaka Establishment and Molecular Phenotyping of Organoids from the Squamocolumnar Junction Region of the Uterine Cervix |
title | Establishment and Molecular Phenotyping of Organoids from the Squamocolumnar Junction Region of the Uterine Cervix |
title_full | Establishment and Molecular Phenotyping of Organoids from the Squamocolumnar Junction Region of the Uterine Cervix |
title_fullStr | Establishment and Molecular Phenotyping of Organoids from the Squamocolumnar Junction Region of the Uterine Cervix |
title_full_unstemmed | Establishment and Molecular Phenotyping of Organoids from the Squamocolumnar Junction Region of the Uterine Cervix |
title_short | Establishment and Molecular Phenotyping of Organoids from the Squamocolumnar Junction Region of the Uterine Cervix |
title_sort | establishment and molecular phenotyping of organoids from the squamocolumnar junction region of the uterine cervix |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140077/ https://www.ncbi.nlm.nih.gov/pubmed/32183493 http://dx.doi.org/10.3390/cancers12030694 |
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