Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs

OBJECTIVES: Endothelial cell injury is a critical pathological change during the development of atherosclerosis. Here, we explored the effect of omentin-1 on free fatty acid- (FFA-) induced endothelial cell injury. METHODS: An FFA-induced endothelial cell injury model was established to investigate...

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Autores principales: Chen, Yubin, Liu, Fen, Han, Fei, Lv, Lizhi, Tang, Can-e, Luo, Fanyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140148/
https://www.ncbi.nlm.nih.gov/pubmed/32300482
http://dx.doi.org/10.1155/2020/3054379
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author Chen, Yubin
Liu, Fen
Han, Fei
Lv, Lizhi
Tang, Can-e
Luo, Fanyan
author_facet Chen, Yubin
Liu, Fen
Han, Fei
Lv, Lizhi
Tang, Can-e
Luo, Fanyan
author_sort Chen, Yubin
collection PubMed
description OBJECTIVES: Endothelial cell injury is a critical pathological change during the development of atherosclerosis. Here, we explored the effect of omentin-1 on free fatty acid- (FFA-) induced endothelial cell injury. METHODS: An FFA-induced endothelial cell injury model was established to investigate the role of omentin-1 in this process. Cell proliferation was analyzed with the Cell Counting Kit assay and flow cytometry. Scratch and transwell assays were used to evaluate cell migration. Factors secreted by endothelial cells after injury were detected by western blotting, reverse-transcription quantitative polymerase chain reaction, and cellular fluorescence assay. RESULTS: Omentin-1 rescued the FFA-induced impaired proliferation and migration capabilities of human umbilical vein endothelial cells (HUVECs). It decreased the number of THP-1 cells attached to HUVECs in response to injury and inhibited the FFA-induced proinflammatory state of HUVECs. CONCLUSION: Omentin-1 could partly ameliorate FFA-induced endothelial cell injury.
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spelling pubmed-71401482020-04-16 Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs Chen, Yubin Liu, Fen Han, Fei Lv, Lizhi Tang, Can-e Luo, Fanyan Cardiol Res Pract Research Article OBJECTIVES: Endothelial cell injury is a critical pathological change during the development of atherosclerosis. Here, we explored the effect of omentin-1 on free fatty acid- (FFA-) induced endothelial cell injury. METHODS: An FFA-induced endothelial cell injury model was established to investigate the role of omentin-1 in this process. Cell proliferation was analyzed with the Cell Counting Kit assay and flow cytometry. Scratch and transwell assays were used to evaluate cell migration. Factors secreted by endothelial cells after injury were detected by western blotting, reverse-transcription quantitative polymerase chain reaction, and cellular fluorescence assay. RESULTS: Omentin-1 rescued the FFA-induced impaired proliferation and migration capabilities of human umbilical vein endothelial cells (HUVECs). It decreased the number of THP-1 cells attached to HUVECs in response to injury and inhibited the FFA-induced proinflammatory state of HUVECs. CONCLUSION: Omentin-1 could partly ameliorate FFA-induced endothelial cell injury. Hindawi 2020-03-27 /pmc/articles/PMC7140148/ /pubmed/32300482 http://dx.doi.org/10.1155/2020/3054379 Text en Copyright © 2020 Yubin Chen et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Yubin
Liu, Fen
Han, Fei
Lv, Lizhi
Tang, Can-e
Luo, Fanyan
Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
title Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
title_full Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
title_fullStr Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
title_full_unstemmed Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
title_short Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
title_sort omentin-1 ameliorated free fatty acid-induced impairment in proliferation, migration, and inflammatory states of huvecs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140148/
https://www.ncbi.nlm.nih.gov/pubmed/32300482
http://dx.doi.org/10.1155/2020/3054379
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