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Sequential i-GONAD: An Improved In Vivo Technique for CRISPR/Cas9-Based Genetic Manipulations in Mice

Improved genome-editing via oviductal nucleic acid delivery (i-GONAD) is a technique capable of inducing genomic changes in preimplantation embryos (zygotes) present within the oviduct of a pregnant female. i-GONAD involves intraoviductal injection of a solution containing genome-editing components...

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Detalles Bibliográficos
Autores principales:	Sato, Masahiro, Miyagasako, Rico, Takabayashi, Shuji, Ohtsuka, Masato, Hatada, Izuho, Horii, Takuro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140409/ https://www.ncbi.nlm.nih.gov/pubmed/32110989 http://dx.doi.org/10.3390/cells9030546

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