Cargando…
Nanosphere Loaded With Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus
Curcumin, a hydrophobic polyphenol of turmeric, has a variety of biological functions as a herbal supplement, but its poor gastric absorption rate is one of the major factors limiting its oral bioavailability. In the present study, we have investigated the functional role of a nanosphere loaded with...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140471/ https://www.ncbi.nlm.nih.gov/pubmed/32151068 http://dx.doi.org/10.3390/cells9030631 |
_version_ | 1783518999031578624 |
---|---|
author | Kim, Ji-Yun Lee, Young-Min Kim, Do-Wan Min, Taesun Lee, Sei-Jung |
author_facet | Kim, Ji-Yun Lee, Young-Min Kim, Do-Wan Min, Taesun Lee, Sei-Jung |
author_sort | Kim, Ji-Yun |
collection | PubMed |
description | Curcumin, a hydrophobic polyphenol of turmeric, has a variety of biological functions as a herbal supplement, but its poor gastric absorption rate is one of the major factors limiting its oral bioavailability. In the present study, we have investigated the functional role of a nanosphere loaded with curcumin (CN) during host cell death elicited by the Gram-negative bacterium V. vulnificus in human gastrointestinal epithelial HT-29 cells and an ileal-ligated mouse model. The recombinant protein (r) VvhA produced by V. vulnificus significantly reduced the viability of HT-29 cells. The cytotoxic effect of rVvhA was restored upon a treatment with CN (100 ng/mL), which had shown 1000-fold higher anti-apoptotic efficacy than curcumin. CN inhibited the phosphorylation of c-Src and PKC mediated by intracellular ROS responsible for the distinctive activation of the MAPKs in rVvhA-treated HT-29 cells. Interestingly, CN significantly restored the expression of Bax, Bcl-2, and cleaved caspase-3 as regulated by the phosphorylation of NF-κB. In mouse models of V. vulnificus infection, treatment with CN had a blocking effect that elevated the levels of TUNEL-positive DNA fragmentation and apoptosis-related proteins. These results indicate that CN is a functional agent that manipulates the V. vulnificus VvhA signaling pathway responsible for gastrointestinal cell death. |
format | Online Article Text |
id | pubmed-7140471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71404712020-04-13 Nanosphere Loaded With Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus Kim, Ji-Yun Lee, Young-Min Kim, Do-Wan Min, Taesun Lee, Sei-Jung Cells Article Curcumin, a hydrophobic polyphenol of turmeric, has a variety of biological functions as a herbal supplement, but its poor gastric absorption rate is one of the major factors limiting its oral bioavailability. In the present study, we have investigated the functional role of a nanosphere loaded with curcumin (CN) during host cell death elicited by the Gram-negative bacterium V. vulnificus in human gastrointestinal epithelial HT-29 cells and an ileal-ligated mouse model. The recombinant protein (r) VvhA produced by V. vulnificus significantly reduced the viability of HT-29 cells. The cytotoxic effect of rVvhA was restored upon a treatment with CN (100 ng/mL), which had shown 1000-fold higher anti-apoptotic efficacy than curcumin. CN inhibited the phosphorylation of c-Src and PKC mediated by intracellular ROS responsible for the distinctive activation of the MAPKs in rVvhA-treated HT-29 cells. Interestingly, CN significantly restored the expression of Bax, Bcl-2, and cleaved caspase-3 as regulated by the phosphorylation of NF-κB. In mouse models of V. vulnificus infection, treatment with CN had a blocking effect that elevated the levels of TUNEL-positive DNA fragmentation and apoptosis-related proteins. These results indicate that CN is a functional agent that manipulates the V. vulnificus VvhA signaling pathway responsible for gastrointestinal cell death. MDPI 2020-03-05 /pmc/articles/PMC7140471/ /pubmed/32151068 http://dx.doi.org/10.3390/cells9030631 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Ji-Yun Lee, Young-Min Kim, Do-Wan Min, Taesun Lee, Sei-Jung Nanosphere Loaded With Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus |
title | Nanosphere Loaded With Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus |
title_full | Nanosphere Loaded With Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus |
title_fullStr | Nanosphere Loaded With Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus |
title_full_unstemmed | Nanosphere Loaded With Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus |
title_short | Nanosphere Loaded With Curcumin Inhibits the Gastrointestinal Cell Death Signaling Pathway Induced by the Foodborne Pathogen Vibrio vulnificus |
title_sort | nanosphere loaded with curcumin inhibits the gastrointestinal cell death signaling pathway induced by the foodborne pathogen vibrio vulnificus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140471/ https://www.ncbi.nlm.nih.gov/pubmed/32151068 http://dx.doi.org/10.3390/cells9030631 |
work_keys_str_mv | AT kimjiyun nanosphereloadedwithcurcumininhibitsthegastrointestinalcelldeathsignalingpathwayinducedbythefoodbornepathogenvibriovulnificus AT leeyoungmin nanosphereloadedwithcurcumininhibitsthegastrointestinalcelldeathsignalingpathwayinducedbythefoodbornepathogenvibriovulnificus AT kimdowan nanosphereloadedwithcurcumininhibitsthegastrointestinalcelldeathsignalingpathwayinducedbythefoodbornepathogenvibriovulnificus AT mintaesun nanosphereloadedwithcurcumininhibitsthegastrointestinalcelldeathsignalingpathwayinducedbythefoodbornepathogenvibriovulnificus AT leeseijung nanosphereloadedwithcurcumininhibitsthegastrointestinalcelldeathsignalingpathwayinducedbythefoodbornepathogenvibriovulnificus |